44 research outputs found
Mutagenesis of ultraviolet-irradiated lambda phage by host cell irradiation: Induction of weigle mutagenesis is not an all-or-none process
Construction and characterization of a plasmid coding for a fragment of the Escherichia coli recA protein
Degradation of Escherichia coli DNA: Evidence for limitation in vivo by protein X, the recA gene product
Influence of plasmids carrying the lexA gene on DNA repair and related processes in Escherichia coli K-12
OCT4 acts as an integrator of pluripotency and signal-induced differentiation
Cell type specification relies on the capacity of undifferentiated cells to properly respond to specific differentiation-inducing signals. Using genomic approaches along with loss- and gain-of-function genetic models, we identified OCT4-dependent mechanisms that provide embryonic stem cells with the means to customize their response to external cues. OCT4 binds a large set of low-accessible genomic regions. At these sites, OCT4 is required for proper enhancer and gene activation by recruiting co-regulators and RAR:RXR or {beta}-catenin, suggesting an unexpected collaboration between the lineage-determining transcription factor and these differentiation-initiating, signal-dependent transcription factors. As a proof of concept, we demonstrate that overexpression of OCT4 in a kidney cell line is sufficient for signal-dependent activation of otherwise unresponsive genes in these cells. Our results uncover OCT4 as an integral and necessary component of signal-regulated transcriptional processes required for tissue-specific responses