6 research outputs found

    Effects of 1−MCP on Storage Quality and Enzyme Activity of Petals of Edible Rose Cultivar ‘Dianhong’ at Low Temperatures

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    To explore the effect of 1-methylcyclopropene (1−MCP) on the storage quality of edible roses, flowers of the edible rose variety ‘Dianhong’ were used as materials to study the effect of post-harvest 1−MCP fumigation (concentration of 30 μL/L). The measures included respiration intensity, water loss rate, antioxidant-related substance content (soluble sugar, crude fibre, AsA, anthocyanin, total phenols and MDA), enzyme activity (SOD, CAT, APX, PAL and PPO) and petal appearance quality in different storage periods, which could provide a theoretical reference for research and development on storage and preservation technology for edible rose petals. The results showed that, compared with the control, 1−MCP treatment reduced the initial respiration intensity of petals by more than 80%, slowed the water loss rate, increased the crude fibre content and effectively maintained the contents of soluble sugars, AsA, anthocyanins and total phenols. For the petals in the treatment group at the end of storage, the rate of water loss was 3.73%, the content of soluble sugar was only 17% (62.27 μg/g)—lower than that of fresh petals—and the content of AsA (0.33 mg/g) was the same as that of pre-storage (0.34 mg/g). The contents of total phenols and anthocyanins were 30.60% and 11.63% higher than those of the control group, respectively. In addition, 1−MCP treatment increased the activity of SOD, CAT, APX and PAL and inhibited the activity of PPO. The MDA content at the end of storage was 14.36% lower than that of the control, which reduced the rate of membrane lipid peroxidation. Correlation analysis showed that sensory quality of petals in the 1−MCP treatment group was positively correlated with respiratory intensity and soluble sugar content and negatively correlated with water loss rate, MDA and crude fibre content (p < 0.05) among the four antioxidant enzymes. APX and PAL were positively correlated with anthocyanin content and total phenols content, respectively. These results confirmed that 1−MCP could effectively maintain the storage quality of edible rose petals by increasing the antioxidant capacity of petals and prolong the storage period of fresh petals to 49 days

    Heterologous Expression of <i>Platycodon grandiflorus PgF3′5′H</i> Modifies Flower Color Pigmentation in Tobacco

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    Flavonoid-3′,5′-hydroxylase (F3′5′H) is the key enzyme for the biosynthesis of delphinidin-based anthocyanins, which are generally required for purple or blue flowers. Previously, we isolated a full-length cDNA of PgF3′5′H from Platycodon grandiflorus, which shared the highest homology with Campanula medium F3′5′H. In this study, PgF3′5′H was subcloned into a plant over-expression vector and transformed into tobacco via Agrobacterium tumefaciens to investigate its catalytic function. Positive transgenic tobacco T0 plants were obtained by hygromycin resistance screening and PCR detection. PgF3′5′H showed a higher expression level in all PgF3′5′H transgenic tobacco plants than in control plants. Under the drive of the cauliflower mosaic virus (CaMV) 35S promoter, the over-expressed PgF3′5′H produced dihydromyricetin (DHM) and some new anthocyanin pigments (including delphinidin, petunidin, peonidin, and malvidin derivatives), and increased dihydrokaempferol (DHK), taxifolin, tridactyl, cyanidin derivatives, and pelargonidin derivatives in PgF3′5′H transgenic tobacco plants by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis, resulting in a dramatic color alteration from light pink to magenta. These results indicate that PgF3′5′H products have F3′5′H enzyme activity. In addition, PgF3′5′H transfer alters flavonoid pigment synthesis and accumulation in tobacco. Thus, PgF3′5′H may be considered a candidate gene for gene engineering to enhance anthocyanin accumulation and the molecular breeding project for blue flowers

    Application of Morphological and Physiological Markers for Study of Drought Tolerance in <i>Lilium</i> Varieties

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    The shortage of water resources is an unfavourable factor that restricts the production of flowers. The use of drought-resistant morphological markers is of great significance to distinguish the drought resistance of flower varieties. In this paper, we study the difference in drought tolerance of seven common lily varieties in the flower market by morphological and physiological markers. The results showed that there were differences in leaf morphological indices and anatomical structures among the seven varieties. Drought reduced the chlorophyll content, inhibited the photosynthetic rate, and increased catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), malondialdehyde (MDA), proline, soluble sugar, and soluble protein. After rewatering, the activities of CAT, POD, and SOD of ‘Lyon’, ‘Royal Sunset’, and ‘Robina’ varieties decreased, which was opposite to the varieties of ‘Immaculate’, ‘Elena’, ‘Siberia’, and ‘Gelria’. According to the membership function value of physiological indices, the drought resistance of seven lily varieties from weak to strong was ‘Immaculate’, ‘Elena’, ‘Siberia’, ‘Gelria’, ‘Robina’, ‘Royal Sunset’, and ‘Lyon’. Drought resistance is related to the thickness of leaves, palisade tissue, sponge tissue, and specific leaf area. Lily leaf structure can be used as one of the indices to judge drought resistance

    Application of Morphological and Physiological Markers for Study of Drought Tolerance in Lilium Varieties

    No full text
    The shortage of water resources is an unfavourable factor that restricts the production of flowers. The use of drought-resistant morphological markers is of great significance to distinguish the drought resistance of flower varieties. In this paper, we study the difference in drought tolerance of seven common lily varieties in the flower market by morphological and physiological markers. The results showed that there were differences in leaf morphological indices and anatomical structures among the seven varieties. Drought reduced the chlorophyll content, inhibited the photosynthetic rate, and increased catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), malondialdehyde (MDA), proline, soluble sugar, and soluble protein. After rewatering, the activities of CAT, POD, and SOD of &lsquo;Lyon&rsquo;, &lsquo;Royal Sunset&rsquo;, and &lsquo;Robina&rsquo; varieties decreased, which was opposite to the varieties of &lsquo;Immaculate&rsquo;, &lsquo;Elena&rsquo;, &lsquo;Siberia&rsquo;, and &lsquo;Gelria&rsquo;. According to the membership function value of physiological indices, the drought resistance of seven lily varieties from weak to strong was &lsquo;Immaculate&rsquo;, &lsquo;Elena&rsquo;, &lsquo;Siberia&rsquo;, &lsquo;Gelria&rsquo;, &lsquo;Robina&rsquo;, &lsquo;Royal Sunset&rsquo;, and &lsquo;Lyon&rsquo;. Drought resistance is related to the thickness of leaves, palisade tissue, sponge tissue, and specific leaf area. Lily leaf structure can be used as one of the indices to judge drought resistance
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