Investigating a Global Collapsing Hub-Filament Cloud G326.611+0.811

We present the dynamics study toward the G326.611+0.811 (G326) hub-filament-system (HFS) cloud using the new APEX observations of both $^{13}$CO and C$^{18}$O (J = 2-1). The G326 HFS cloud constitutes a central hub and at least four hub-composing filaments that are divided into a major branch of filaments (F1, and F2) and a side branch (F3-F5). The cloud holds ongoing high-mass star formation as characterised by three massive dense clumps (i.e., 370-1100 $M_{\odot}$ and 0.14-0.16 g cm$^{-2}$ for C1-C3) with the high clump-averaged mass infalling rates ($>10^{-3}$ $M_{\odot}$ yr$^{-1}$) within in the major filament branch, and the associated point sources bright at 70 $\mu$m typical of young protostars. Along the five filaments, the velocity gradients are found in both $^{13}$CO and C$^{18}$O (J = 2-1) emission, suggesting that the filament-aligned gravitational collapse toward the central hub (i.e., C2) is being at work for high-mass star formation therein. Moreover, a periodic velocity oscillation along the major filament branch is revealed in both $^{13}$CO and C$^{18}$O (J = 2-1) emission with a characteristic wavelength of $\sim$3.5 pc and an amplitude of $\sim$0.31-0.38 km s$^{-1}$. We suggest that this pattern of velocity oscillation in G326 could arise from the clump-forming gas motions induced by gravitational instability. Taking into account the prevalent velocity gradients, the fragmentation of the major branch of filaments, and the ongoing collapse of the three massive dense clumps, it is indicative that G326 is a HFS undergoing global collapse.Comment: 21 pages, 13 figures, 2 tables, Accepted for publication in Ap

Progress and hotspot of diet or exercise therapy in the treatment of non-alcoholic fatty liver disease

IntroductionThe primary treatment for non-alcoholic fatty liver disease (NAFLD) is modifying lifestyle through dietary or exercise interventions. In recent decades, it has received increasing attention. However, the lack of bibliometric analysis has posed a challenge for researchers seeking to understand the overall trends in this field.MethodsAs of February 3rd, 2024, 876 articles on treating NAFLD through diet or exercise therapy from 2013 to 2023 had been retrieved. Two software tools, VOSviewer and CiteSpace, were utilized to analyze the growth of publications, countries, institutions, authors, journals, citations, and keywords. Additionally, the keywords with strong citation burstiness were identified to determine the changes and future trends of research hotspots in this field.ResultsChina had the highest number of articles, followed by the United States and South Korea. Yonsei University and Nutrients were the institutions and journals with the most significant contributions. Professor Younossi Zobair M, from the United States, is the most prolific author in this field. Through analyzing the keywords, three research hotspots were identified: research on the pathogenesis of NAFLD, research on the treatment modalities of NAFLD, and research on the risk factors and diagnosis methods of NAFLD. In recent years, the research emphasis in this field has changed, suggesting that future research will focus on two frontier keywords: “oxidative stress” and “aerobic capacity.”ConclusionIn the past eleven years, the attention in this field was still rising, and the authors, journals, countries and so on had formed a considerable cooperative relationship. There were also many highly influential and productive researchers in this field. It is speculated that new research will continue around “aerobic exercise” and “oxidative stress” in the future

A cytomegalovirus peptide-specific antibody alters natural killer cell homeostasis and ss shared in several autoimmune diseases

Human cytomegalovirus (hCMV), a ubiquitous beta-herpesvirus, has been associated with several autoimmune diseases. However, the direct role of hCMV in inducing autoimmune disorders remains unclear. Here we report the identification of an autoantibody that recognizes a group of peptides with a conserved motif matching the Pp150 protein of hCMV (anti-Pp150) and is shared among patients with various autoimmune diseases. Anti-Pp150 also recognizes the single-pass membrane protein CIP2A and induces the death of CD56bright NK cells, a natural killer cell subset whose expansion is correlated with autoimmune disease. Consistent with this finding, the percentage of circulating CD56bright NK cells is reduced in patients with several autoimmune diseases and negatively correlates with anti-Pp150 concentration. CD56bright NK cell death occurs via both antibody- and complement-dependent cytotoxicity. Our findings reveal that a shared hCMV-induced autoantibody is involved in the decrease of CD56bright NK cells and may thus contribute to the onset of autoimmune disorders

An Efficient Intergeneric Conjugation of DNA from Escherichia coli to Mycelia of the Lincomycin-Producer Streptomyces lincolnensis

Streptomyces lincolnensis is a producer of lincomycin, which is a lincosamide antibiotic for the treatment of infective diseases caused by Gram-positive bacteria. S. lincolnensis is refractory to introducing plasmid DNA into cells because of resistance of foreign DNAs and poor sporulation. In this study, a simple and efficient method of transferring plasmids into S. lincolnensis through the intergeneric Escherichia coli-mycelia conjugation was established and optimized for the first time. The recipient mycelia of S. lincolnensis were prepared in liquid SM medium containing 10.3% sucrose for three days. The dispersed mycelia were conjugated with competent E. coli donor cells. The exconjugants were regenerated efficiently on solid mannitol soya flour (MS) medium containing 20 mM MgCl2. The average conjugation frequency was observed at 1.1 × 10−4 per input donor cell and validated functionally by transferring two types of vectors containing lincomycin resistance genes lmrA, lmrB and lmrC into S. lincolnensis mycelia. The data of fermentation in shaking flasks showed the lincomycin yield of the exconjugants increased by 52.9% for the multiple copy vector and 38.3% for the integrative one, compared with the parental strain. The efficient and convenient method of intergeneric E. coli-mycelia conjugation in this study provides a promising procedure to introduce plasmid DNA into other refractory streptomycetes