Web Shopping Expert Systems Using New Interval Type-2 Fuzzy Reasoning

Finding a product with high quality and reasonable price online is a difficult task due to the fuzzy nature of data and queries. In order to handle the fuzzy problem, a new type-2 fuzzy reasoning based decision support system, the Web Shopping Expert for online users is proposed. In the Web Shopping Expert, an interval type-2 fuzzy logic system is used and a fuzzy output can be obtained using the up-low limit technique, which offers an opportunity to directly employ all the rules and methods of the type-1 fuzzy sets onto the type-2 fuzzy sets. To achieve the best performance the fuzzy inference system is optimized by the least square and numerical method. The key advantages of the least square method are the efficient use of samples and the simplicity of the implementation. The Web Shopping Expert based on the interval type-2 fuzzy inference system provides more reasonable conclusions for online users

Non-viral delivery and optimized optogenetic stimulation of retinal ganglion cells led to behavioral restoration of vision

Stimulation of retinal neurons using optogenetics via use of chanelrhodopsin-2 (ChR2) has opened up a new direction for restoration of vision for treatment of retinitis pigmentosa (RP). Here, we report non-viral in-vivo electroporation of degenerated retina of adult RP-mice with ChR2-plasmids and subsequent in-vivo imaging of retina to confirm expression. Further, we demonstrate that in addition to efficient non-viral delivery of ChR2 to a specific retinal layer, threshold level of stimulation light needs to be delivered onto the retina for achieving successful behavioral outcome. Measurement of intensity of light reaching the retina of RP-mouse models along with geometrical optics simulation of light propagation in the eye is reported in order to determine the stimulating source position for optimal light delivery to the retina. The light-guided navigation of mice with ChR2 expressing retinal ganglion cells was found to be significantly improved over a long distance in correlation with stimulation intensity