Evaluation of the Sterility of PRP Obtained with Multi-bag System (PRPBAG®)

Introduction:The aim of this study is demonstrating the sterility of PRPBAG®, a multi-bag completely closed PRP preparation system different than PRP kits in the market and determining preservability of the sterility of PRP produced with PRPBAG® up to 5 days similar to other platelet products.Methods:We recruited 60 participants for increased statistical significance. 150 mL of whole blood was collected in specially produced PRPBAG®. A double-spin preparation method was used for the samples with a refrigerated centrifuge specially manufactured for PRPBAG®. We started centrifugation within 1 h after collecting blood. In the 1st spin of the centrifugation, whole blood was centrifuged. Erythrocytes precipitated in the first bag because of blood component density, and the supernatant plasma was transferred into the second bag using a manual plasma extractor. The third bag was empty after the first spin. We used a hose-closing device to separate the first bag, which contained erythrocytes, from the other bags. In the second spin of centrifugation, the plasma was centrifuged.Results:PRP product with high-quality was collected in the second bag. We have considered the European guidelines for the preparation, use, and quality assurance of blood components during the whole process. 1 cc of PRP was added into separate pediatric blood culture bottles on days 0, 1, 2, 3, 4, and 5. These samples were sent to the microbiology laboratory and incubated for 5 days. After this incubation period, the blood culture bottles that did not give positive signals were reported as sterile.Conclusion:Currently, PRP is used immediately after preparation. Our study demonstrates that PRP prepared with a closed triple blood bag system PRPBAG® may be used for 5 days if agitated at room temperature. There was not any bacterial growth in any sample after 5 days

The rredictive value of R wave peak time to detect thrombus burden in st-segment elevation myocardial infarction: a retrospective cohort study in a tertiary medical center

Background: Patients with higher thrombus burden have higher procedural complications and more long-term adverse cardiac events. Detecting patients with high thrombus burden (HTB) before coronary intervention could help avoid procedural complications. Objective: The research aimed to analyze the R wave peak time (RWPT) on the electrocardiogram to predict thrombus burden before coronary angiography in patients with acute ST-segment elevation myocardial infarction (STEMI). Materials and Methods: A total of 159 patients with STEMI were included in the study conducted at a tertiary medical center. The thrombolysis in myocardial infarction (TIMI) thrombus scale was applied to assess the thrombus burden. TIMI thrombus grades 0, 1, 2, and 3 were accepted as low; 4 and 5 had HTB. RWPT was measured from the beginning of the QRS complex to the R-peak from the leads pointing to the infarct-related artery. Results: Patients were divided into two groups according to their angiographically defined thrombus burden as low and high. The low thrombus burden group (LTB) comprised fifty-four patients, whereas the HTB group comprised 105 patients. In the LTB group, RWPT was 47.96 ± 9.17 ms, and in the HTB group was 53.58 ± 8.92 ms; it was significantly longer (p 46.5 ms predicted the occurrence of HTB with a sensitivity and specificity of 87.62% and 51.85%, respectively (AUC 0.682, 95% CI 0.590-0.774, p < 0.001). Conclusion: The present study evaluated the relationship between the RWPT and thrombus burden in STEMI patients. Based on the results, RWPT is an independent predictor of HTB