Antimicrobial agents resistance in Campylobacter coli from swine and humans

C. coli from slaughtered pigs and from human patients were examined for resistance to quinolones and tetracycline. Detection of tetO was performed. Gyrase A gene (gyrA) was amplified and sequenced and tested by an alternative method. Tetracycline resistance levels were respectively of 67,7 % and 56,3 % in swine and human isolates. In C. coli of human origin, all resistant isolates had the tetO determinant while 82,8 % of resistant C. coli from swine possessed it. Among the susceptible swine isolates, 17,2 % possessed this gene. Resistance to enrofloxacin (7,3 %) and ciprofloxacin (11,4 %) was observed in swine isolates and resistance for enrofloxacin (12,5 %) and ciprofloxacin (18,8 %) were observed in C. coli from humans. In addition, 72,7 % of swine resistant isolates and all isolates from humans had a mutation at position 86. Results were similar with MAMA-PCR which can thus be considered as a good alternative to sequencing

Antimicrobial Agent susceptibility of campyolobacter and salmonella from swine herds with various therapeutic regimens

Fecal samples were collected in pens from 27 farrow-to-finish swine herds using (n = 10) and not using fluoroquinolones (n = 17) and in herds using (n = 20) or not (n = 7) tetracycline. Workers on the farms participated to the study. Salmonella was found in 4 out of the 27 sampled herds and were all resistant to tetracycline and susceptible to enrofloxacin. No Salmonella and no Campylobacter were isolated from human. C. coli was found in all sampled herds with an average of 68,5 % positive pens. In farms not using quinolones and tetracycline, no resistance was observed among 2 herds but resistance to enrofloxacin (71 %) and to tetracycline (100 %) were observed in some farms. In herds where tetracycline was used, resistance levels varied from 7 % to 100 %. Resistance levels to quinolones in herds using this agent varied from 0 % to 100 %

Antimicrobial susceptibility patterns of eampylobacter coli isolates from swine and humans

An increase in resistance to various antimicrobial agents has been reported in many countries for Campylobacter species. In order to study their resistance profiles to various antimicrobial agents, strains ofC. coli isolated from slaughtered pigs and from human patients were examined for resistance to quinolones and other antimicrobial agents by agar dilution technique. Resistance to tetracycline was common for human (53 %) and swine (68 %) isolates. Higher level of resistance to streptomycine was found in swine isolates (67 %) than in human (24 %) isolates. Human isolates were more frequently found to be resistant to enrofloxacin and ciprofloxacin (18 and 24 %) than swine isolates (7 and II %). Sixty one percent C. coli from swine were resistant to erythromycine while only 12 %of C. coli of human origin were resistant. Emergence of resistance to macrolides, tetracyclines and quinolones is of interest in regard with possible dissemination of genetic resistance determinants to humans

Genotyping of Campylobacter isolates from swine, poultry and humans in Canada.

Direct epidemiological evidence indicating that pork meat can be a source of campylobacteriosis in human are missing, while it is widely accepted for poultry products. A total of I 01 Campylobacter strains isolated from slaughtered swines, 195 strains isolated from broilers as well as 24 human Campylobacter strains from ill patients were included in this study. The genetic characterization of isolates was performed by pulse-field gel electrophoresis (PFGE). Macrorestrictions profiles were obtained with by digestion of DNA withKpni. The combination of the 72 PFGE swine patterns with the PFGE human patterns gave no genetic link between strains while 20 % of human isolates were genetically related to poultry isolates. Since C. jejuni was mainly recovered from human and poultry while swine harbored C. coli, the role of swine in the transmission of Campylobacter to human population by meat consumption remain uncertain

Risk factors associated with presence of Salmonella in pigs in Canada

Carriers of Salmonella in finishing pigs are believed to be a main source of carcass and pork contamination at the slaughterhouse and during meat processing. To better control the presence of Salmonella at the farm and slaughter, it is important to adequately identify on-farm risk factors associated with presence of these bacteria in animals

Antimicrobial Agent susceptibility of campyolobacter and salmonella from swine herds with various therapeutic regimens

Fecal samples were collected in pens from 27 farrow-to-finish swine herds using (n = 10) and not using fluoroquinolones (n = 17) and in herds using (n = 20) or not (n = 7) tetracycline. Workers on the farms participated to the study. Salmonella was found in 4 out of the 27 sampled herds and were all resistant to tetracycline and susceptible to enrofloxacin. No Salmonella and no Campylobacter were isolated from human. C. coli was found in all sampled herds with an average of 68,5 % positive pens. In farms not using quinolones and tetracycline, no resistance was observed among 2 herds but resistance to enrofloxacin (71 %) and to tetracycline (100 %) were observed in some farms. In herds where tetracycline was used, resistance levels varied from 7 % to 100 %. Resistance levels to quinolones in herds using this agent varied from 0 % to 100 %.</p

Antimicrobial agents resistance in Campylobacter coli from swine and humans

C. coli from slaughtered pigs and from human patients were examined for resistance to quinolones and tetracycline. Detection of tetO was performed. Gyrase A gene (gyrA) was amplified and sequenced and tested by an alternative method. Tetracycline resistance levels were respectively of 67,7 % and 56,3 % in swine and human isolates. In C. coli of human origin, all resistant isolates had the tetO determinant while 82,8 % of resistant C. coli from swine possessed it. Among the susceptible swine isolates, 17,2 % possessed this gene. Resistance to enrofloxacin (7,3 %) and ciprofloxacin (11,4 %) was observed in swine isolates and resistance for enrofloxacin (12,5 %) and ciprofloxacin (18,8 %) were observed in C. coli from humans. In addition, 72,7 % of swine resistant isolates and all isolates from humans had a mutation at position 86. Results were similar with MAMA-PCR which can thus be considered as a good alternative to sequencing.</p

Genotyping of Campylobacter isolates from swine, poultry and humans in Canada.

Direct epidemiological evidence indicating that pork meat can be a source of campylobacteriosis in human are missing, while it is widely accepted for poultry products. A total of I 01 Campylobacter strains isolated from slaughtered swines, 195 strains isolated from broilers as well as 24 human Campylobacter strains from ill patients were included in this study. The genetic characterization of isolates was performed by pulse-field gel electrophoresis (PFGE). Macrorestrictions profiles were obtained with by digestion of DNA withKpni. The combination of the 72 PFGE swine patterns with the PFGE human patterns gave no genetic link between strains while 20 % of human isolates were genetically related to poultry isolates. Since C. jejuni was mainly recovered from human and poultry while swine harbored C. coli, the role of swine in the transmission of Campylobacter to human population by meat consumption remain uncertain.</p

Prevalence and genetic characterization of Campylobacter spp. isolates from swine in Quebec, Canada

In order to assess the importance of swine in the transmission of Campylobacter, it is relevant to evaluate its prevalence and distribution in the animal population and to further characterize this microorganism. A total of 850 animals were screened at slaughterhouse for the presence of Campylobacter.</p

Antimicrobial susceptibility patterns of eampylobacter coli isolates from swine and humans

An increase in resistance to various antimicrobial agents has been reported in many countries for Campylobacter species. In order to study their resistance profiles to various antimicrobial agents, strains ofC. coli isolated from slaughtered pigs and from human patients were examined for resistance to quinolones and other antimicrobial agents by agar dilution technique. Resistance to tetracycline was common for human (53 %) and swine (68 %) isolates. Higher level of resistance to streptomycine was found in swine isolates (67 %) than in human (24 %) isolates. Human isolates were more frequently found to be resistant to enrofloxacin and ciprofloxacin (18 and 24 %) than swine isolates (7 and II %). Sixty one percent C. coli from swine were resistant to erythromycine while only 12 %of C. coli of human origin were resistant. Emergence of resistance to macrolides, tetracyclines and quinolones is of interest in regard with possible dissemination of genetic resistance determinants to humans.</p