Chemical composition and antifungal activity of Salvia sclarea (Lamiaceae) essential oil

Clary sage (Salvia sclarea L.) is native to Southern Europe and is cultivated worldwide. The essential oil of clary sage was analyzed as a potential antifungal agent. The main compounds in the oil were linalyl acetate (52.83%) and linalool (18.18%). Food poisoning agents, spoilage fungi, and plant and animal pathogens were among the tested fungal species. The microdilution method was used to establish minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC). The commercial antimycotic bifonazole was used as a control. A concentration of 25 μl/ml showed fungicidal activity against Aspergillus, Penicillium, and Fusarium species and Trichoderma viride. For the species Mucor mucedo and Aspergillus viride, the MFC was 15μl/ml; for Candida albicans, it was 10 μl/ml, as in the case of bifonazole. Fungistatic and fungicidal activities of the oil against Cladosporium cladosporioides and Trichophyton menthagrophytes were recorded at concentrations of 2.5 μl/ml and 5 μl/ml. The most sensitive micromycetes were Cladosporium fulvum, Alternaria alternata, Phomopsis helianthi, and Phoma macdonaldii, where a concentration of 2.5 μl/ml was lethal.Šarlahna žalfija (Salvia sclarea L.) je kao samonikla vrsta rasprostranjena u Južnoj Evropi, a kultivisana širom sveta. U radu je analizirano etarsko ulje ove vrste i utvrđivana njegova antifungalna aktivnost. Glavne komponente etarskog ulja su linalil acetat (52.83%) i linalol (18.18%). Kao test organizmi korišćene su gljivice koje izazivaju kvarenje hrane, kao i patogeni biljaka i životinja. Korišćenjem mikrodilucione metode određivane su minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije (MFC). Komercijalni antimikotik bifonazol je korišćen kao kontrola. U koncentraciji od 25 µl/ml ulje je delovalo fungicidno na vrste rodova Aspergillus, Penicillium i Fusarium i vrstu Trichoderma viride. Za vrste Mucor mucedo i Aspergillus viride MFC je bila 15 µl/ml, i 10 µl/ml za C. albicans, slično kao za bifonazol. Potpuno zaustavljanje rasta micelija Cladosporium cladosporioides i Trichophyton menthagrophytes je postignuto pri koncentracijama 2.5 µl/ml i 5 µl/ml. Najosetljivije mikromicete bile su Cladosporium fulvum, Alternaria alternata, Phomopsis helianthi i Phoma macdonaldii, za koje je letalna koncentracija bila 2.5 µl/ml.Projekat ministarstva br. 143041 i 14304

Chemical composition and antifungal activity of Salvia sclarea (Lamiaceae) essential oil

Clary sage (Salvia sclarea L.) is native to Southern Europe and is cultivated worldwide. The essential oil of clary sage was analyzed as a potential antifungal agent. The main compounds in the oil were linalyl acetate (52.83%) and linalool (18.18%). Food poisoning agents, spoilage fungi, and plant and animal pathogens were among the tested fungal species. The microdilution method was used to establish minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC). The commercial antimycotic bifonazole was used as a control. A concentration of 25 μl/ml showed fungicidal activity against Aspergillus, Penicillium, and Fusarium species and Trichoderma viride. For the species Mucor mucedo and Aspergillus viride, the MFC was 15μl/ml; for Candida albicans, it was 10 μl/ml, as in the case of bifonazole. Fungistatic and fungicidal activities of the oil against Cladosporium cladosporioides and Trichophyton menthagrophytes were recorded at concentrations of 2.5 μl/ml and 5 μl/ml. The most sensitive micromycetes were Cladosporium fulvum, Alternaria alternata, Phomopsis helianthi, and Phoma macdonaldii, where a concentration of 2.5 μl/ml was lethal.Šarlahna žalfija (Salvia sclarea L.) je kao samonikla vrsta rasprostranjena u Južnoj Evropi, a kultivisana širom sveta. U radu je analizirano etarsko ulje ove vrste i utvrđivana njegova antifungalna aktivnost. Glavne komponente etarskog ulja su linalil acetat (52.83%) i linalol (18.18%). Kao test organizmi korišćene su gljivice koje izazivaju kvarenje hrane, kao i patogeni biljaka i životinja. Korišćenjem mikrodilucione metode određivane su minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije (MFC). Komercijalni antimikotik bifonazol je korišćen kao kontrola. U koncentraciji od 25 µl/ml ulje je delovalo fungicidno na vrste rodova Aspergillus, Penicillium i Fusarium i vrstu Trichoderma viride. Za vrste Mucor mucedo i Aspergillus viride MFC je bila 15 µl/ml, i 10 µl/ml za C. albicans, slično kao za bifonazol. Potpuno zaustavljanje rasta micelija Cladosporium cladosporioides i Trichophyton menthagrophytes je postignuto pri koncentracijama 2.5 µl/ml i 5 µl/ml. Najosetljivije mikromicete bile su Cladosporium fulvum, Alternaria alternata, Phomopsis helianthi i Phoma macdonaldii, za koje je letalna koncentracija bila 2.5 µl/ml.Projekat ministarstva br. 143041 i 14304

Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin–Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 μmol Fe/mg. The best radical scavenger was methanol (1.04 μmol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties

Chemical composition and antifungal activity of Origanum heracleoticum essential oil

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Antimicrobial activity of essential oil of juniperus communis l.

Composition of the essential oil of Juniperus communis L. and its potential antimicrobial activity were analyzed. Composition of the essential oil was analysed by GC and GC/MS. The major components were a-pinene (40.33%), b-myrcene (13.72%), limonene (5.61%) and sabinene (5.38%). As test organisms the following micromycetes Penicillium ochrochloron, Aspergillus niger, Trichoderma viride and bacteria Bacillus subtilis, Staphylococcus epidermidis, Escherichia coli and Micrococcus luteus were used. Microdilution method on microplates 96-system was used for detection of minimum inhibition concentration (MIC), minimum bactericidal (MBC) and fungicidal concentration (MFC). Essential oil did not show antibacterial activity against E. coli, and for B. subtilis and M. luteus it was low. The oil of J. communis exhibited low antifungal activity against tested micromycetes.U ovom radu je analiziran hemijski sastav etarskog ulja obične kleke (Juniperus communis L.) i njegovo antimikrobno (antibakterijsko i antifungalno) dejstvo. Za utvrđivanje sastava etarskog ulja kleke korišćeni su GC i GC/MS. Najzastupljenije komponente u ovom ulju su a-pinen (40.33%), b-mircen (13.72%), limonen (5.61%) i sabinen (5.38%). Test organizmi korišćeni u eksperimentu su bile mikromicete: Penicillium ochrochloron, Aspergillus niger, Trichoderma viride i bakterijski sojevi Bacillus subtilis, Staphylococcus epidermidis, Escherichia coli i Micrococcus luteus. Metodom mikrodilucije na mikrotitracionim pločama, utvrđivane su minimalne inhibitorne i mikrobicidne koncentracije (MIC i MMC). Etarsko ulje je pokazalo aktivnost na ispitivane mikromicete na višim koncentracijama. Etarsko ulje je na B. subtilis i M. luteus pokazalo slabiju aktivnost dok na bakteriju E. coli nije delovalo.nul

The chemical composition of some Lauraceae essential oils and their antifungal activities

The antifungal activity of Aniba rosaeodora, Laurus nobilis, Sassafras albidum and Cinnamomum zeylanicum essential oils were investigated against 17 micromycetes. Among the tested fungal species were food poisoning, spoilage fungi, plant and animal pathogens. In order to determine fungistatic and fungicidal concentrations (MIC and MFC) macrodilution and microdilution tests were used. Linalool was the main component in the essential oil of A. rosaeodora, while 1.8-cineole was dominant in L. nobilis. In sassafras essential oil safrole was the major component and in the oil of C. zeylanicum the main component was trans-cinnamaldehyde. The essential oil of cinnamon showed the strongest antifungal activity. Copyright (C) 2004 John Wiley Sons, Ltd.nul

Chemical composition and antifungal activity of Illicium verum and Eugenia caryophyllata essential oils

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Antimicrobial activity of essential oil of melissa officinalis l. - Lamiaceae

Commercial sample of essential oil of M. officinalis was analysed using GC and GC/MS. The main compounds identified in the essential oil were citronellal (29.3%), limonen (8.84%), geranial (8.70%), a-terpineole (8.02%), a-terpinyl acetate (7.14%) and neral (5.68%). As test organisms the following micromycetes Penicillium ochrochloron, Aspergillus niger Trichoderma viride and bacteria Bacillus subtilis, Staphylococcus epidermidis, Escherichia coli and Micrococcus luteus were used. Microdilution method on microplates 96-system was used for detection of minimum inhibition concentration (MIC), minimum bactericidal (MBC) and fungicidal concentration (MFC). Essential oil did not show antibacterial effect, but the oil of M. officinalis exhibited significant antifungal activity against tested micromycetes.Analiziran je hemijski sastav komercijalnog uzorka etarskog ulja matičnjaka (Melissa officnalis L) i njegova antibakterijska i antifungalna aktivnost. GC i GC-MS analizom konstatovane su najzastupljenije komponente: citronelal (29.3%), limonen (8.84%), geranial (8.70%), a-terpineol (8.02%), a-terpinil acetat (7.14%) i nerol (5.68%). Za ispitivanje biološke aktivnosti korišćeni su test organizmi, mikromicete: Aspergillus niger, Penicillium ochrochloron Trichoderma viride i bakterijski sojevi: Bacillus subtilis, Escherichia coli, Micrococcus luteus i Staphylococcus epidermidis. Mikrodilucionom metodom ispiti-vane su minimalne inhibitorne i mikrobicidne koncentracije. Etarsko ulje matičnjaka je pokazalo značajnu antifungalnu aktivnost ali nizak baktericidni efekat.nul

Chemical composition and antifungal activity of Illicium verum and Eugenia caryophyllata essential oils

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Chemical composition and antifungal activity of Origanum heracleoticum essential oil

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