Processing and Transmission of Information

Contains reports on three research projects.Lincoln Laboratory, Purchase Order DDL B-00368U. S. ArmyU. S. NavyU. S. Air Force under Air Force Contract AF19(604)-7400National Institutes of Health (Grant MH-04737-03)National Science Foundation (Grant G-16526

Processing and Transmission of Information

Contains reports on two research projects.Lincoln Laboratory (Purchase Order DDL-B-00306)United States ArmyUnited States NavyUnited States Air Force (Contract AF19(604)-5200

Processing and Transmission of Information

Contains reports on four research projects.Lincoln Laboratory (Purchase Order B-00306)United States ArmyUnited States NavyUnited States Air Force (Contract AF19(604)-7400

Processing and Transmission of Information

Contains reports on four research projects.Lincoln Laboratory, Purchase Order DDL B-00306U. S. ArmyU. S. NavyU. S. Air Force under Air Force Contract AF19(604)-520

Processing and Transmission of Information

Contains research objectives and reports on three research projects.National Science Foundation (Grant G-16526)National Institutes of Health (Grant MH-04737-03)National Aeronautics and Space Administration (Grant NsG-496)Lincoln Laboratory, Purchase Order DDL BB-107U.S. Air Force under Contract AF 19(628)-50

Processing and Transmission of Information

Contains research objectives and reports on four research projects.Lincoln Laboratory, Purchase Order DDL B-00306U. S. ArmyU. S. NavyU. S. Air Force under Air Force Contract AF19(604)-7400National Science Foundation (Grant B-16526)National Institutes of Health (Grant MP-4737

Cognitive Information Processing

Contains research objectives and summary of research on fourteen research projects and reports on four research projects.Joint Services Electronics Program (Contract DAAB07-75-C-1346)National Science Foundation (Grant EPP74-12653)National Science Foundation (Grant ENG74-24344)National Institutes of Health (Grant 2 PO1 GM19428-04)Swiss National Funds for Scientific ResearchM.I.T. Health Sciences Fund (Grant 76-11)National Institutes of Health (Grant F03 GM58698)National Institutes of Health (Biomedical Sciences Support Grant)Associated Press (Grant

Efficient generation of gene knockout plasmids for Dictyostelium discoideum using one-step cloning.

The amoeba Dictyostelium discoideum is a well-established model organism for studying numerous aspects of cellular and developmental functions. Its rather small (~34Mb) chromosomal genome and the high efficiency of gene disruption by homologous recombination have enabled researchers to dissect various specific gene functions. We describe here the use of one-step cloning for the fast and efficient generation of deletion vectors that are produced in a one-step reaction by inserting two PCR products into an organism-specific, generic acceptor system. This worked efficiently for all 16 tested constructs directed against genes in the amoeba Dictyostelium discoideum. Saving cost and time, the used protocol represents a significant advancement in the generation of such plasmids compared to the conventionally applied restriction enzyme/ligation approach. Using appropriate selection markers, similar systems could also be useful in other organisms, where genes can be knocked out by homologous recombination