Genetic diversity of phytoplasmas associated with flavescence dorée at vineyard scale on the Ischia Island (Campania, Italy)

Flavescence dorée (FD) is the most important grapevine yellows, constituting a great threat in all major viticultural areas in Europe. The FD causal agent is an incidentally cited phytoplasma species termed ‘Candidatus Phytoplasma vitis’ (FDp), listed as a quarantine organism in Europe and mainly transmitted from vine to vine by the monophagous leafhopper Scaphoideus titanus. In the present study, the genetic variability of FDp identified in symptomatic plants located in a vineyard cv. Biancolella on the Ischia Island (Campania, Italy) was investigated. Our results unveiled for the first time the presence of FDp strains belonging to the subgroup 16SrV–C with a low genetic variability within map and rp genetic markers, reflecting an homogeneous genetic lineage of phytoplasma population examined in the present study. Phylogeny and nucleotide sequence analyses can also suggest the possible involvement of other insect vectors and plant hosts in the FD epidemiology on the Island. Further investigation to ascertain the presence of putative vectors and plant hosts representing inoculum sources should be carried out to reinforce the preliminary results obtained in the present study

The vaulting system of the Palatine Chapel: the Aachen Cathedral world heritage site documentation project.

As part of a comprehensive survey and modelling project involving the Aachen Cathedral, this paper focuses on its oldest part, the Palatine Chapel, a domed octagonal hall supported by eight piers and enveloped by a sixteen-sided outer wall. Working on the data collected during an extensive 3D capturing campaign conducted between 2022 and 2023, this paper will focus on the conic vaults covering the ambulacrum of the 1st floor that represent quite a peculiar architectural and structural solution considering the VIII/IX century building know-how. In this framework, the Chapel's 3D point cloud has been analysed to extract the main 2D generative elements of the conic surfaces and then construct the corresponding 3D geometric models. These outputs have been compared against the captured point cloud to assess the differences between the actual vault data and the reconstructed ideal conic shapes. Finally, the method used to unfold the vaults' surfaces and create high-resolution ortho-images has been displayed

Vecchi scavi e nuove tecnologie: primi risultati del Progetto Basilica Iulia

The paper presents the first results of the Basilica Julia Project, aimed at the analysis and reconstruction of the transformations affecting the southern side of the Forum Romanum. In the excavation campaigns conducted between 1960 and 1964 beneath the Augustan building, the remains of the basilica of Julius Caesar, the Basilica Sempronia (169 BC), a large house with an ‘atrium’ dated to the 4th cent. BC, and traces of a 5th cent. BC building were brought to light. During the first phase of the work, the stratigraphic sequences were reconstructed according to the archive documentation and the study of the archaeological materials. In the second phase, a wide-ranging survey campaign was undertaken. The paper examines the methods of acquisition, connected to conservation demands, the lack of natural light and the complex physical configuration of the site. Surveying was undertaken by integrating topographical survey with massive data collection through 3D Laser Scanner and Structure from Motion. The paper also focuses on the process of integration of all these different data-sets into a single model, which produces a considerable quantity of information already translated into a unique system. Finally, thanks to the possibility to explore the 3D model in the restitution phase, the model made it possible to produce a 2D plan and sections in a process that ensured the correct reading of the data

An innovative approach for the semantic segmentation of surveyed building facades leveraging on architectural drawings

Traditionally, drawing products created from 3D surveying activities have been the universal medium of communication used by architects. This has resulted in a vast repository of graphic documentation that serves as a testament of the architectural heritage. The embedded information found in elevations, plans and sections holds considerable value, and it can be seamlessly integrated into the intricate graphics produced during large-scale data acquisition processes. The core objective of this research is to investigate how the information coming from the large amount of existing architectural technical drawings can support 3D heritage classification processes and avoid time-consuming annotation of materials and construction techniques of historical building facades. Starting from available sets of drawings, AI-based methodologies are applied for the annotation of orthoimages and point clouds in order to obtain a predictive model that can recognize classes of materials and construction techniques in a large amount of data. The predicted classes also allow the automatic creation of vector drawing representing the facades of new buildings, providing a novel tool to facilitate the processes of analysis and conservation of architectural heritage

Smartphone-based particle tracking velocimetry for the in vitro assessment of coronary flows

The present study adopts a smartphone-based approach for the experimental characterization of coronary flows. Technically, Particle Tracking Velocimetry (PTV) measurements were performed using a smartphone camera and a low-power continuous wave laser in realistic healthy and stenosed phantoms of left anterior descending artery with inflow Reynolds numbers approximately ranging from 20 to 200. A Lagrangian-Eulerian mapping was performed to convert Lagrangian PTV velocity data to a Eulerian grid. Eulerian velocity and vorticity data obtained from smartphone-based PTV measurements were compared with Particle Image Velocimetry (PIV) measurements performed with a smartphone-based setup and with a conventional setup based on a high-power double-pulsed laser and a CMOS camera. Smartphone-based PTV and PIV velocity flow fields substantially agreed with conventional PIV measurements, with the former characterized by lower average percentage differences than the latter. Discrepancies emerged at high flow regimes, especially at the stenosis throat, due to particle image blur generated by smartphone camera shutter speed and image acquisition frequency. In conclusion, the present findings demonstrate the feasibility of PTV measurements using a smartphone camera and a low-power light source for the in vitro characterization of cardiovascular flows for research, industrial and educational purposes, with advantages in terms of costs, safety and usability

EVOLUTION OF RECORDING METHODS: THE AACHEN CATHEDRAL WORLD HERITAGE SITE DOCUMENTATION PROJECT

Modern terrestrial laser scanners and photogrammetric imaging systems can provide highly accurate and objective as-built records of existing architectural, engineering, and industrial sites. This comprehensive digital recording benefits culturally significant places like heritage buildings, monuments, and other vital structures. The collected data can be instrumental in various ways, including aiding in conservation, management, monitoring and repair efforts and serving as an educational resource for scholars and the general public. These technical capabilities are especially well-suited for architecturally complex, ornate buildings like the Aachen Cathedral UNESCO World Heritage site. This paper describes the recent recording efforts at the Aachen Cathedral and is a comparative study of the previous documentation work done at the Cologne Cathedral.The 3D documentation of the Aachen Cathedral UNESCO World Heritage Site is an ongoing collaborative project between the Sapienza Università di Roma, Rome, Italy, Robert Gordon University, Aberdeen, Scotland, and in partnership with RWTH Aachen University, and the Dombauhütte Aachen.</p

An oily fish diet improves subclinical inflammation in people at high cardiovascular risk: A randomized controlled study

Interest has arisen on the anti-inflammatory action of dietary components, including long-chain n-3 fatty acids (LCn3) and polyphenols (PP). The aim of this study was to evaluate the effects of diets rich in PP and oily fish (high-LCn3 diets) on markers of subclinical inflammation and growth factors in people at high cardiometabolic risk. Individuals with high waist circumference and one more component of metabolic syndrome were randomized to one of the following isoenergetic diets: Low LCn3&amp;PP, high LCn3, high PP, high LCn3&amp;PP. Before and after 8 weeks, fasting and postprandial plasma concentrations of hs-CRP and fasting serum concentrations of IL-1, IL-4, IL-6, IL-10, IL-17, INF-, TNF-, FGF, VEGF, PDGF-, G-CSF, and GM-CSF were determined. An oily fish diet reduced fasting plasma hs-CRP (1.28 ± 12.0, −12.5 ± 6.9, 22.5 ± 33.6, −12.2 ± 11.9; 8-week percent change, Mean ± SEM; low LCn3&amp;PP, high LCn3, high PP, high LCn3&amp;PP group, respectively), postprandial 6h-AUC hs-CRP (4.6 ± 16.3, −18.2 ± 7.2, 26.9 ± 35.1, −11.5 ± 11.8, 8-week percent change) and fasting IL-6 (20.8 ± 18.7, −2.44 ± 12.4, 28.1 ± 17.4, −9.6 ± 10.2), IL-17 (2.40 ± 4.9, −13.3 ± 4.9, 3.8 ± 4.43, −11.5 ± 4.7), and VEGF (−5.7 ± 5.8, −5.6 ± 7.5, 3.5 ± 5.8, −11.1 ± 5.5) (8-week percent change; p &lt; 0.05 for LCn3 effect for all; no significant effect for PP; 2-factor ANOVA). An oily fish diet improved subclinical inflammation, while no significant effect was observed for dietary polyphenols

Development of a loop-mediated isothermal amplification (LAMP) assay for the identification of the invasive wood borer Aromia bungii (Coleoptera: Cerambycidae) from frass

The red-necked longhorn beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) is native to east Asia, where it is a major pest of cultivated and ornamental species of the genus Prunus. Morphological or molecular discrimination of adults or larval specimens is required to identify this invasive wood borer. However, recovering larval stages of the pest from trunks and branches causes extensive damage to plants and is timewasting. An alternative approach consists in applying non-invasive molecular diagnostic tools to biological traces (i.e., fecal pellets, frass). In this way, infestations in host plants can be detected without destructive methods. This paper presents a protocol based on both real-time and visual loop-mediated isothermal amplification (LAMP), using DNA of A. bungii extracted from fecal particles in larval frass. Laboratory validations demonstrated the robustness of the protocols adopted and their reliability was confirmed performing an inter-lab blind panel. The LAMP assay and the qPCR SYBR Green method using the F3/B3 LAMP external primers were equally sensitive, and both were more sensitive than the conventional PCR (sensitivity > 103 to the same starting matrix). The visual LAMP protocol, due to the relatively easy performance of the method, could be a useful tool to apply in rapid monitoring of A. bungii and in the management of its outbreaks

Active front-end rectifier modelling using dynamic phasors for more-electric aircraft applications

The More-Electric Aircraft (MEA) has become a dominant trend for next-generation aircraft. The Electrical Power System (EPS) on-board may take many forms: AC, DC, hybrid, frequency-wild, variable voltage, together with the possibility of novel connectivity topologies. To address the stability, availability and capability issues as well as to assess the performance of the power quality and transient behaviour, extensive simulation work is required to develop the EPS architectures. The paper develops a fast-simulation model of active front-end rectifiers based on the dynamic phasor concept. The model is suitable for accelerated simulation studies of EPS under normal, unbalanced and line fault conditions. The performance and effectiveness of the developed model have been demonstrated by comparison against time-domain models in three-phase and synchronous space-vector representations. The experimental verification of the dynamic phasor model is also reported. The prime purpose of the model is for the simulation studies of MEA power architectures at system level; however it can be directly applied for simulation study of any other EPS interfacing with active front-end rectifiers

A First Search for coincident Gravitational Waves and High Energy Neutrinos using LIGO, Virgo and ANTARES data from 2007

We present the results of the first search for gravitational wave bursts associated with high energy neutrinos. Together, these messengers could reveal new, hidden sources that are not observed by conventional photon astronomy, particularly at high energy. Our search uses neutrinos detected by the underwater neutrino telescope ANTARES in its 5 line configuration during the period January - September 2007, which coincided with the fifth and first science runs of LIGO and Virgo, respectively. The LIGO-Virgo data were analysed for candidate gravitational-wave signals coincident in time and direction with the neutrino events. No significant coincident events were observed. We place limits on the density of joint high energy neutrino - gravitational wave emission events in the local universe, and compare them with densities of merger and core-collapse events.Comment: 19 pages, 8 figures, science summary page at http://www.ligo.org/science/Publication-S5LV_ANTARES/index.php. Public access area to figures, tables at https://dcc.ligo.org/cgi-bin/DocDB/ShowDocument?docid=p120000