Evaluation of floor vibrations induced by walking in reinforced concrete buildings

Floor vibrations induced by human walking were investigated in a reinforced concrete structure. Six experimental floor structures were built in laboratories with the same dimensions and boundary conditions. Subjective tests were performed to assess the vibration serviceability of the floor structures. First, the subjects were asked to walk across a floor and then to rate the intensity of the vibrations, acceptability, and serviceability of the floors. In the second part of the tests, the subjects were seated on a chair placed in the middle of the floor and asked to rate floor vibrations when the walker passed the subjects. Floor vibrations induced by human walking were analyzed using peak acceleration, root mean square (r.m.s.) acceleration, and the vibration dose value (VDV), and four weighting functions (Wb, Wk, Wg, and Wm) were applied. Significant differences in the measured floor vibration were found across the floor structures, larger floor vibration lead to greater perceived vibration intensity, lower acceptability and serviceability. The Wb and Wk were found to be more applicable than Wg and Wm to explain perception of floor vibration. It was observed that the impact noise induced by walking did not influence the evaluation of floor vibratio

Maternal obesity is associated with the formation of small dense LDL and hypoadiponectinemia in the third trimester

Context: Maternal obesity is associated with high plasma triglyceride, poor vascular function, and an increased risk for pregnancy complications. In normal-weight pregnant women, higher triglyceride is associated with increased small, dense low-density lipoprotein (LDL). Hypothesis: In obese pregnancy, increased plasma triglyceride concentrations result in triglyceride enrichment of very low-density lipoprotein-1 particles and formation of small dense LDL via lipoprotein lipase. Design: Women (n = 55) of body mass index of 18–46 kg/m2 were sampled longitudinally at 12, 26, and 35 weeks' gestation and 4 months postnatally. Setting: Women were recruited at hospital antenatal appointments, and study visits were in a clinical research suite. Outcome Measures: Plasma concentrations of lipids, triglyceride-rich lipoproteins, lipoprotein lipase mass, estradiol, steroid hormone binding globulin, insulin, glucose, leptin, and adiponectin were determined. Results: Obese women commenced pregnancy with higher plasma triglyceride, reached the same maximum, and then returned to higher postnatal levels than normal-weight women. Estradiol response to pregnancy (trimester 1–3 incremental area under the curve) was positively associated with plasma triglyceride response (r2 adjusted 25%, P < .001). In the third trimester, the proportion of small, dense LDL was 2-fold higher in obese women than normal-weight women [mean (SD) 40.7 (18.8) vs 21.9 (10.9)%, P = .014], and 35% of obese, 14% of overweight, and none of the normal-weight women displayed an atherogenic LDL subfraction phenotype. The small, dense LDL mass response to pregnancy was inversely associated with adiponectin response (17%, P = .013). Conclusions: Maternal obesity is associated with an atherogenic LDL subfraction phenotype and may provide a mechanistic link to poor vascular function and adverse pregnancy outcome

Characterisation of the entomopathogenic nematode Heterorhabditis (Nematoda : Heterorhabditidae) from Ireland and Britain by molecular and cross-breeding techniques, and the occurrence of the genus in these islands

Des examens de sol ont été effectués en Irlande et en Grande-Bretagne pour rechercher les nématodes du genre #Heterorhabditis. Des échantillons des sols prélevés sur des sites sablonneux furent pourvus de larves de #Galleria mellonella comme appâts. Des #Heterorhabditis furent collectés sur 18 sites sur 169 en Irlande, 2 sur 51 dans le nord de l'Ecosse et 9 sur 20 dans le sud du Pays de Galle. Tous les sites positifs sont situés sur le littoral ; aucun #Heterorhabditis n'a été détecté dans les échantillons prélevés dans 40 sites situés à l'intérieur des terres. La totalité des 76 isolats recueillis au cours de ces analyses ont été identifiés comme appartenant au groupe irlandais d'#Heterorhabditis, à l'aide de méthodes de concentration isoélectrique, de restriction de l'ADN et d'hybridation. Aucun #Heterohabditis appartenant au groupe de l'Europe du nord-ouest n'a été recueilli. Cependant, un isolat recueilli par d'autres chercheurs et provenant du sud de l'Angleterre a été identifié comme appartenant à ce groupe. Les membres du groupe irlandais ne se croisent généralement pas avec les membres du groupe de l'Europe du nord-ouest, bien que des juvéniles infestants fertiles aient été produits dans un nombre limité (3/15) de croisements entre ces groupes. (Résumé d'auteur

Mechanisms of Corticotropin-Releasing Factor (CRF) Gene Regulation in the Frog Xenopus laevis.

The vertebrate hypothalamic-pituitary-adrenal/interrenal (HPA/HPI) axis plays a central role in integrating the stress response and maintaining homeostasis. My dissertation research focused on understanding the regulatory mechanisms of the key regulator of the stress axis, the corticotropin-releasing factor (CRF). I took a comparative/evolutionary approach to this problem, and utilized the South African clawed frog Xenopus laevis as a model system. I found that the general distribution of CRF expression in the frog brain are highly conserved with other vertebrates. Similar to mammals, exposure of juvenile frogs to a stressor caused rapid activation of CRF neurons in the anterior preoptic area (POA), which is homologous to the mammalian paraventricular nucleus (PVN) and the primary site of neuroendocrine control of the HPA/HPI axis. The CRF neurons in the limbic structure the medial amygdala (MeA) and bed nucleus of the stria terminalis (BNST) were also activated following exposure to the stressor. I found that the structures and sequences of the CRF genes are highly conserved among tetrapods. Using a comparative genomic approach, I identified putative transcription factor binding sites in the proximal promoters of the frog CRF genes. I then tested the functionality of these sites by cell transfection, in vitro binding assays, chromatin precipitation (ChIP) assays, and in vivo electroporation-mediated gene transfer. I showed that a conserved cAMP response element (CRE) in the CRF promoter mediates gene regulation by the PKA pathway in vitro, and stressor-dependent activation in vivo. I also found that the distribution of the glucocorticoid receptor (GR) in the frog CNS is highly conserved with mammals. Similar to mammals, I showed that glucocorticoids down-regulate CRF expression in the POA; whereas they up-regulate it in the MeA and BNST. Overall, my research showed that the cell-specific expression within the CNS, stressor-dependent activation, feedback regulation, and transcriptional regulatory mechanisms of CRF genes are highly conserved among the tetrapods. My discoveries suggest that the basic regulatory mechanisms and neuronal circuits of the CRF system arose before the divergence of the amphibian and amniote lineages, and have been conserved by strong positive selection.Ph.D.Molecular, Cellular, and Developmental BiologyUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/57594/3/yaom_1.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/57594/2/yaom_2.pd

Damped Bogoliubov excitations of a condensate interacting with a static thermal cloud

We calculate the damping of condensate collective excitations at finite temperatures arising from the lack of equilibrium between the condensate and thermal atoms. We neglect the non-condensate dynamics by fixing the thermal cloud in static equilibrium. We derive a set of generalized Bogoliubov equations for finite temperatures that contain an explicit damping term due to collisional exchange of atoms between the two components. We have numerically solved these Bogoliubov equations to obtain the temperature dependence of the damping of the condensate modes in a harmonic trap. We compare these results with our recent work based on the Thomas-Fermi approximation.Comment: 9 pages, 3 figures included. Submitted to PR

Scaling Flows and Dissipation in the Dilute Fermi Gas at Unitarity

We describe recent attempts to extract the shear viscosity of the dilute Fermi gas at unitarity from experiments involving scaling flows. A scaling flow is a solution of the hydrodynamic equations that preserves the shape of the density distribution. The scaling flows that have been explored in the laboratory are the transverse expansion from a deformed trap ("elliptic flow"), the expansion from a rotating trap, and collective oscillations. We discuss advantages and disadvantages of the different experiments, and point to improvements of the theoretical analysis that are needed in order to achieve definitive results. A conservative bound based on the current data is that the minimum of the shear viscosity to entropy density ration is that eta/s is less or equal to 0.5 hbar/k_B.Comment: 32 pages, prepared for "BCS-BEC crossoverand the Unitary Fermi Gas", Lecture Notes in Physics, W. Zwerger (editor), Fig. 5 corrected, note added; final version, corrected typo in equ. 9

The identification of biological species in the genus Heterorhabditis (Nematoda : Heterorhabditidae) by cross-breeding second-generation amphimictic adults

Entomopathogenic nematodes of the genus Heterorhabditis are morphologically conservative, consequently the majority of isolates remain unassigned at the species level. When a Heterorhabditis infective juvenile infects an insect host, it develops into a protandrous hermaphrodite female. These first-generation females give rise to a second generation which contains both males and females. Because of this complex life-cycle and also because of uncertainty as to whether second-generation females are amphimictic, cross-breeding studies to facilitate species determination have not been carried out previously. We demonstrate here that second-generation Heterorhabditis females are amphimictic. Because of this finding, we have been able to develop a successful cross-breeding technique for the purposes of species determination in Heterorhabditis. Interstrain crosses using second-generation males and females from the appropriate strains have been successfully set up in Xenorhabdus luminescens-treated G. mellonella cadavers and also on agar plates. Using the techniques described here we confirm that H . bacteriophora (Brecon strain), H . megidis and H . zealandica are distinct biological species, we note that the H. bacteriophora group contains at least 2 species and we provide evidence for the existence of a new Irish species of Heterorhabditis

Finite temperature effects on the collapse of trapped Bose-Fermi mixtures

By using the self-consistent Hartree-Fock-Bogoliubov-Popov theory, we present a detailed study of the mean-field stability of spherically trapped Bose-Fermi mixtures at finite temperature. We find that, by increasing the temperature, the critical particle number of bosons (or fermions) and the critical attractive Bose-Fermi scattering length increase, leading to a significant stabilization of the mixture.Comment: 5 pages, 4 figures; minor changes, proof version, to appear in Phys. Rev. A (Nov. 1, 2003