Intravital FRAP imaging using an E-cadherin-GFP mouse reveals disease- and drug-dependent dynamic regulation of cell-cell junctions in live tissue

E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments

Comparative efficiency of domestic hot water systems

2. ed.; Reports supersede nos 57800/1 and 57800/2, pub. Aug 1989; Work carried out for Waterheater Manufacturers AssociationSIGLEAvailable from British Library Document Supply Centre- DSC:7389.20(BSRIA--57800/1-2) / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Comparative efficiency of domestic hot water systems

2. ed.; Reports supersede nos 57800/1 and 57800/2, pub. Aug 1989; Work carried out for Waterheater Manufacturers AssociationSIGLEAvailable from British Library Document Supply Centre- DSC:7389.20(BSRIA--57800/1-2) / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Managing to Learn: the Social Poetics of a Polyphonic 'Classroom'

This paper draws on Bakhtin’s use of Polyphony and explores it potential for organising processes within management education. In developing the concept of a polyphonic ‘classroom’, the interplay between tutor, manager-student and theory is related to Bakhtin’s identification of the relationship between hero, other characters and idea within Dostoevsky’s novels. In particular, a carnivalesque polyphonic relations is argued to change tutor-student relations, extend the physical classroom into a wider polyphonic ‘classroom’ that includes the manager-student’s work context and re-imagines learning as a changing, social poetic performance beyond common understanding of learning as cognitive processes of understanding or sense making