The Great School Legend

Two quotes from Colin Greer\u27s The Great School Legend, A Revisionist Interpretation of American Public Education

Divergent functional traits in three sympatric Arctic charr Salvelinus alpinus morphs are not coupled with the age of the lineage divergence

Three genetically discrete morphs of Arctic charr in Loch Rannoch, Scotland originated from a recent divergence within the lake (in situ) (piscivore and benthivore morphs) and from secondary contact of two older lineages (ex situ; a planktivore–piscivore/benthivore divergence). To test if the expression of traits with strong functional roles was linked to the age of the divergence, fin and gill anatomy, and dentition were quantified and compared across morphs. Five additional working hypotheses suggesting a rank order of trait expression amongst morphs were also tested. The planktivorous morph had more rays in the dorsal and pectoral fins, longer gill rakers (but not more) as well as a smaller gill cavity than the other two morphs. The piscivorous morph had more palatine teeth and longer teeth on the mandible, pre-maxillary and glossohyal bones, and a larger buccal cavity. These differences indicate a differential response to selection in these functional anatomical features most likely related to morph foraging specialisms. Notably, between-morph divergences in the expression of these traits were not simply linked to the length of divergence between morphs and have arisen equally quickly in the recent (in situ) divergence as they have in older, ex situ divergences

Paleosalinity History of Middle Holocene Lagoonal and Lacustrine Deposits in the Enriquillo Valley, Dominican Republic Based on Pore Morphometrics and Isotope Geochemistry of Ostracoda

The southwestern region of the Dominican Republic (Enriquillo Valley) contains exceptionally well-preserved, relict marine and saline lake deposits of mid-Holocne age. Abundant euryhaline ostracodes found in this deposit include Cyprideis salebrosa, C. mexicana, C. similis, and C. edentata. Morphometric and geochemical analyses performed on Cyprideis spp. provide high δ18O and δ13C values that are coincident with relative abundances of irregularly shaped pores that permeate the ostracode carapace. We recognize three stratigraphic intervals with distinct ostracode pore shape and stable isotope trends: (I) a 4.5-5.0m interval that contains ostracodes with highly irregular shaped pores (multiradiate) and high amplitude variability in δ18O and δ13C values; (II) a 5.0-5.6m interval comprised of ostracodes with circular pores and an overall trend towards low δ18O and δ13C values; and (III) a 5.6-6.5m interval containing ostracodes with an upward increasing abundance of circular pore shapes coincident with decreasing δ18O and δ13C values. When the Enriquillo lagoon was first separated from the Caribbean Sea approximately 4.3 ka, an arid and evaporative climate led to hypersaline water in a restricted lagoon environment. By the middle to late-Holocene, increased precipitation in the valley resulted in a coastal lake system that became progressively oligohaline. Moderate to small amplitude variability in the salinity proxy data (δ18O) suggest short-term oscillations in the precipitation-evaporation budgets at that time. At least two marine incursions likely contributed to the ob- served variability in ostracode δ18O and δ13C values. Evidence for abrupt changes in base level indicate that climatic factors or also tectonic activity may have contributed to the observed paleoenvironmental trends recorded in these deposits

Renal Cystic Disease Proteins Play Critical Roles in the Organization of the Olfactory Epithelium

It was reported that some proteins known to cause renal cystic disease (NPHP6; BBS1, and BBS4) also localize to the olfactory epithelium (OE), and that mutations in these proteins can cause anosmia in addition to renal cystic disease. We demonstrate here that a number of other proteins associated with renal cystic diseases – polycystin 1 and 2 (PC1, PC2), and Meckel-Gruber syndrome 1 and 3 (MKS1, MKS3) – localize to the murine OE. PC1, PC2, MKS1 and MKS3 are all detected in the OE by RT-PCR. We find that MKS3 localizes specifically to dendritic knobs of olfactory sensory neurons (OSNs), while PC1 localizes to both dendritic knobs and cilia of mature OSNs. In mice carrying mutations in MKS1, the expression of the olfactory adenylate cyclase (AC3) is substantially reduced. Moreover, in rats with renal cystic disease caused by a mutation in MKS3, the laminar organization of the OE is perturbed and there is a reduced expression of components of the odor transduction cascade (Golf, AC3) and α-acetylated tubulin. Furthermore, we show with electron microscopy that cilia in MKS3 mutant animals do not manifest the proper microtubule architecture. Both MKS1 and MKS3 mutant animals show no obvious alterations in odor receptor expression. These data show that multiple renal cystic proteins localize to the OE, where we speculate that they work together to regulate aspects of the development, maintenance or physiological activities of cilia

Cortico-amygdalar connectivity and externalizing/internalizing behavior in children with neurodevelopmental disorders

Background: Externalizing and internalizing behaviors contribute to clinical impairment in children with neurodevelopmental disorders (NDDs). Although associations between externalizing or internalizing behaviors and cortico-amygdalar connectivity have been found in clinical and non-clinical pediatric samples, no previous study has examined whether similar shared associations are present across children with different NDDs. Methods: Multi-modal neuroimaging and behavioral data from the Province of Ontario Neurodevelopmental Disorders (POND) Network were used. POND participants aged 6–18 years with a primary diagnosis of autism spectrum disorder (ASD), attention-deficit/hyperactivity disorder (ADHD) or obsessive–compulsive disorder (OCD), as well as typically developing children (TDC) with T1-weighted, resting-state fMRI or diffusion weighted imaging (DWI) and parent-report Child Behavioral Checklist (CBCL) data available, were analyzed (total n = 346). Associations between externalizing or internalizing behavior and cortico-amygdalar structural and functional connectivity indices were examined using linear regressions, controlling for age, gender, and image-modality specific covariates. Behavior-by-diagnosis interaction effects were also examined. Results: No significant linear associations (or diagnosis-by-behavior interaction effects) were found between CBCL-measured externalizing or internalizing behaviors and any of the connectivity indices examined. Post-hoc bootstrapping analyses indicated stability and reliability of these null results. Conclusions: The current study provides evidence towards an absence of a shared linear relationship between internalizing or externalizing behaviors and cortico-amygdalar connectivity properties across a transdiagnostic sample of children with different primary NDD diagnoses and TDC. Different methodological approaches, including incorporation of multi-dimensional behavioral data (e.g., task-based fMRI) or clustering approaches may be needed to clarify complex brain-behavior relationships relevant to externalizing/internalizing behaviors in heterogeneous clinical NDD populations

Temporal stability of polymorphic Arctic charr parasite communities reflects sustained divergent trophic niches

Polymorphic Arctic charr Salvelinus alpinus populations frequently display distinct differences in habitat use, diet, and parasite communities. Changes to the relative species densities and composition of the wider fish community have the potential to alter the habitat niche of sympatric Arctic charr populations. This study evaluated the temporal stability of the parasite community, diet, and stable isotopes (δ13C, δ15N) of three sympatric Arctic charr morphs (piscivore, benthivore, and planktivore) from Loch Rannoch, Scotland, in relation to changes to the fish community. All Arctic charr morphs displayed distinct differences in parasite communities, diet, and stable isotope signatures over time, despite the establishment of four new trophically transmitted parasite taxa, and increased fish and zooplankton consumption by the piscivorous and planktivore morphs, respectively. Native parasite prevalence also increased in all Arctic charr morphs. Overall, Loch Rannoch polymorphic Arctic charr morph populations have maintained their distinct trophic niches and parasite communities through time despite changes in the fish community. This result indicates that re-stocking a native fish species has the potential to induce shifts in the parasite community and diet of Arctic charr morphs

A Molten Salt Lithium-Oxygen Battery

Despite the promise of extremely high theoretical capacity (2Li + O_2 ↔ Li_2O_2, 1675 mAh per gram of oxygen), many challenges currently impede development of Li/O_2 battery technology. Finding suitable electrode and electrolyte materials remains the most elusive challenge to date. A radical new approach is to replace volatile, unstable and air-intolerant organic electrolytes common to prior research in the field with alkali metal nitrate molten salt electrolytes and operate the battery above the liquidus temperature (>80 °C). Here we demonstrate an intermediate temperature Li/O_2 battery using a lithium anode, a molten nitrate-based electrolyte (e.g., LiNO_3–KNO_3 eutectic) and a porous carbon O_2 cathode with high energy efficiency (∼95%) and improved rate capability because the discharge product, lithium peroxide, is stable and moderately soluble in the molten salt electrolyte. The results, supported by essential state-of-the-art electrochemical and analytical techniques such as in situ pressure and gas analyses, scanning electron microscopy, rotating disk electrode voltammetry, demonstrate that Li_2O_2 electrochemically forms and decomposes upon cycling with discharge/charge overpotentials as low as 50 mV. We show that the cycle life of such batteries is limited only by carbon reactivity and by the uncontrolled precipitation of Li_2O_2, which eventually becomes electrically disconnected from the O_2 electrode

Altered Metabolism and Persistent Starvation Behaviors Caused by Reduced AMPK Function in Drosophila

Organisms must utilize multiple mechanisms to maintain energetic homeostasis in the face of limited nutrient availability. One mechanism involves activation of the heterotrimeric AMP-activated protein kinase (AMPK), a cell-autonomous sensor to energetic changes regulated by ATP to AMP ratios. We examined the phenotypic consequences of reduced AMPK function, both through RNAi knockdown of the gamma subunit (AMPKγ) and through expression of a dominant negative alpha (AMPKα) variant in Drosophila melanogaster. Reduced AMPK signaling leads to hypersensitivity to starvation conditions as measured by lifespan and locomotor activity. Locomotor levels in flies with reduced AMPK function were lower during unstressed conditions, but starvation-induced hyperactivity, an adaptive response to encourage foraging, was significantly higher than in wild type. Unexpectedly, total dietary intake was greater in animals with reduced AMPK function yet total triglyceride levels were lower. AMPK mutant animals displayed starvation-like lipid accumulation patterns in metabolically key liver-like cells, oenocytes, even under fed conditions, consistent with a persistent starved state. Measurements of O2 consumption reveal that metabolic rates are greater in animals with reduced AMPK function. Lastly, rapamycin treatment tempers the starvation sensitivity and lethality associated with reduced AMPK function. Collectively, these results are consistent with models that AMPK shifts energy usage away from expenditures into a conservation mode during nutrient-limited conditions at a cellular level. The highly conserved AMPK subunits throughout the Metazoa, suggest such findings may provide significant insight for pharmaceutical strategies to manipulate AMPK function in humans

Internal validation of STRmix™ – A multi laboratory response to PCAST

We report a large compilation of the internal validations of the probabilistic genotyping software STRmix™. Thirty one laboratories contributed data resulting in 2825 mixtures comprising three to six donors and a wide range of multiplex, equipment, mixture proportions and templates. Previously reported trends in the LR were confirmed including less discriminatory LRs occurring both for donors and non-donors at low template (for the donor in question) and at high contributor number. We were unable to isolate an effect of allelic sharing. Any apparent effect appears to be largely confounded with increased contributor number