Nutrient Removal by Tidal Fresh and Oligohaline Marshes in a Chesapeake Bay Tributary

Located at the interface between estuaries and surrounding uplands, tidal marshes are in position to receive and transform material from both adjacent systems. Of particular importance in eutrophic estuarine systems, tidal marshes permanently remove nutrients via two mechanisms - denitrification and long-term burial. Denitrification was measured (monthly) in two marshes in a Chesapeake Bay tributary for 7 months, using the MIMS technique. Burial of nitrogen (N) and phosphorus (P) was measured using 210Pb techniques. Strong spatial and temporal patterns emerged, and there was a Michaelis-Menten type response in denitrification rates to experimentally elevated nitrate levels. Denitrification rates measured may account for removal of 22% of N inputs to the upper estuary on an annual basis. Burial rates could account for 30% of N inputs and 60% of P inputs. Based on the cost of nutrient control technologies, Patuxent marsh nutrient removal may be valued at $10 to 30 million yr-1

Refining the M_BH-V_c scaling relation with HI rotation curves of water megamaser galaxies

Black hole - galaxy scaling relations provide information about the coevolution of supermassive black holes and their host galaxies. We compare the black hole mass - circular velocity (MBH - Vc) relation with the black hole mass - bulge stellar velocity dispersion (MBH - sigma) relation, to see whether the scaling relations can passively emerge from a large number of mergers, or require a physical mechanism, such as feedback from an active nucleus. We present VLA H I observations of five galaxies, including three water megamaser galaxies, to measure the circular velocity. Using twenty-two galaxies with dynamical MBH measurements and Vc measurements extending to large radius, our best-fit MBH - Vc relation, log MBH = alpha + beta log(Vc /200 km s^-1), yields alpha = 7.43+/-0.13, beta = 3.68+1.23/-1.20, and intrinsic scatter epsilon_int = 0.51+0.11/-0.09. The intrinsic scatter may well be higher than 0.51, as we take great care to ascribe conservatively large observational errors. We find comparable scatter in the MBH - sigma relations, epsilon_int = 0.48+0.10/-0.08, while pure merging scenarios would likely result in a tighter scaling with the dark halo (as traced by Vc) than baryonic (sigma) properties. Instead, feedback from the active nucleus may act on bulge scales to tighten the MBH - sigma relation with respect to the MBH - Vc relation, as observed.Comment: 27 pages, 15 figures, ApJ accepte

A microbial carbonate response in synchrony with the end-Triassic mass extinction across the SW UK

The eruption of the Central Atlantic Magmatic Province (CAMP)—the largest igneous province known—has been linked to the end-Triassic mass extinction event, however reconciling the response of the biosphere (at local and nonlocal scales) to potential CAMP-induced geochemical excursions has remained challenging. Here we present a combined sedimentary and biological response to an ecosystem collapse in Triassic-Jurassic strata of the southwest United Kingdom (SW UK) expressed as widely distributed carbonate microbialites and associated biogeochemical facies. The microbialites (1) occur at the same stratigraphic level as the mass extinction extinction, (2) host a negative isotope excursion in δ(13)C(org) found in other successions around the world, and (3) co-occur with an acme of prasinophyte algae ‘disaster taxa’ also dominant in Triassic-Jurassic boundary strata of other European sections. Although the duration of microbialite deposition is uncertain, it is likely that they formed rapidly (perhaps fewer than ten thousand years), thus providing a high-resolution glimpse into the initial carbon isotopic perturbation coincident with the end-Triassic mass extinction. These findings indicate microbialites from the SW UK capture a nonlocal biosedimentary response to the cascading effects of massive volcanism and add to the current understanding of paleoecology in the aftermath of the end-Triassic extinction

Clostridium difficile infection in the United States: A national study assessing preventive practices used and perceptions of practice evidence

We surveyed 571 US hospitals about practices used to prevent Clostridium difficile infection (CDI). Most hospitals reported regularly using key CDI prevention practices, and perceived their strength of evidence as high. The largest discrepancy between regular use and perceived evidence strength occurred with antimicrobial stewardship programs.Infect. Control Hosp. Epidemiol. 2015;36(8):969–971</jats:p

Human urine decreases function and expression of type 1 pili in uropathogenic Escherichia coli

Uropathogenic Escherichia coli (UPEC) is the primary cause of community-acquired urinary tract infections (UTIs). UPEC bind the bladder using type 1 pili, encoded by the fim operon in nearly all E. coli. Assembled type 1 pili terminate in the FimH adhesin, which specifically binds to mannosylated glycoproteins on the bladder epithelium. Expression of type 1 pili is regulated in part by phase-variable inversion of the genomic element containing the fimS promoter, resulting in phase ON (expressing) and OFF (nonexpressing) orientations. Type 1 pili are essential for virulence in murine models of UTI; however, studies of urine samples from human UTI patients demonstrate variable expression of type 1 pili. We provide insight into this paradox by showing that human urine specifically inhibits both expression and function of type 1 pili. Growth in urine induces the fimS phase OFF orientation, preventing fim expression. Urine also contains inhibitors of FimH function, and this inhibition leads to a further bias in fimS orientation toward the phase OFF state. The dual effect of urine on fimS regulation and FimH binding presents a potential barrier to type 1 pilus-mediated colonization and invasion of the bladder epithelium. However, FimH-mediated attachment to human bladder cells during growth in urine reverses these effects such that fim expression remains ON and/or turns ON. Interestingly, FimH inhibitors called mannosides also induce the fimS phase OFF orientation. Thus, the transduction of FimH protein attachment or inhibition into epigenetic regulation of type 1 pilus expression has important implications for the development of therapeutics targeting FimH function

The Local Nanohertz Gravitational-Wave Landscape From Supermassive Black Hole Binaries

Supermassive black hole binaries (SMBHBs) in the 10 million to 10 billion $M_\odot$ range form in galaxy mergers, and live in galactic nuclei with large and poorly constrained concentrations of gas and stars. There are currently no observations of merging SMBHBs--- it is in fact possible that they stall at their final parsec of separation and never merge. While LIGO has detected high frequency GWs, SMBHBs emit GWs in the nanohertz to millihertz band. This is inaccessible to ground-based interferometers, but possible with Pulsar Timing Arrays (PTAs). Using data from local galaxies in the 2 Micron All-Sky Survey, together with galaxy merger rates from Illustris, we find that there are on average $91\pm7$ sources emitting GWs in the PTA band, and $7\pm2$ binaries which will never merge. Local unresolved SMBHBs can contribute to GW background anisotropy at a level of $\sim20\%$, and if the GW background can be successfully isolated, GWs from at least one local SMBHB can be detected in 10 years.Comment: submitted to Nature Astronomy (reformatted for arXiv

"you just look at rocks, and have beards" Perceptions of geology from the UK: a qualitative analysis from an online survey.

In the last few decades, Geology courses, particularly in the Global North, have seen a decline in student enrolment. Geologists have linked this downturn to a lack of exposure to the subject at school and college level. This work seeks to understand the public's relationship with Geology and draws on over 5000 open-ended question responses to a survey disseminated in 2021. The survey asked both those who had, and had not, studied geology as a subject a series of questions in order to explore their perceptions of the discipline. Our findings indicate that individuals 'outside' of geology see the subject as old fashioned, boring, and environmentally damaging; simply the study of rock samples with nothing new to be discovered from; and with poor job prospects outside of the oil and gas industry. Geologists who responded to the survey paint a picture of a broad, interdisciplinary subject, with vibrant employability opportunities yet struggle to coherently and collectively describe this when asked, 'what is geology?'. In addition to the identified perception of geology as boring, and notions of poor employability being a barrier to prospective students, diversity and inclusivity issues are highlighted as significant barriers by those who study geology. Our findings indicate that both geologists and the geology curriculum need to coherently describe what geology is more effectively. We need to develop and better communicate the subject's interdisciplinary nature and links to critical societal issues, such as the role of responsible mineral extraction in the energy transition and the importance of geology in vital areas such as climate change science, water resource management, environmental conservation, and sustainable urban/built development. Finding new ways to show that, far from being boring, geology is a subject that can fundamentally change the way you see and interact with the world around you is of central importance to achieving this. Efforts to make the subject more equitable are also highlighted as being critical in creating a more inclusive and accessible discipline

IgG4 antibodies to the recombinant filarial antigen Wb-Bhp-1 decrease dramatically following treatment of lymphatic filariasis

BACKGROUND: Lymphatic filariasis (LF) is a neglected tropical disease and a major cause of chronic disability. Improved diagnostic tests are needed because of long-term persistence of anti-filarial antibodies or circulating filarial antigenemia after treatments that clear microfilaremia. Here, we assess changes in levels of antibodies to the recombinant filarial antigens Wb-Bhp-1, Wb123, and Bm14 after anti-filarial treatment. METHODOLOGY/PRINCIPAL FINDINGS: IgG4 antibodies to recombinant filarial antigens were assessed by ELISA. We tested serial plasma samples from a clinical trial in Papua New Guinea. Before treatment, 90%, 71% and 99% of participants had antibodies to Wb-Bhp-1, Wb123, and Bm14, respectively. Antibodies to Wb-Bhp-1 and Wb123, but not Bm14, were significantly higher in participants with persistent microfilaremia 24 months after treatment. Antibodies to all three antigens declined significantly by 60 months after treatment with ivermectin, diethylcarbamazine and albendazole despite circulating filarial antigen in 76% of participants. By 60 months follow up, antibodies to Wb-Bhp-1, Wb123, and Bm14 were detected in 17%, 7% and 90% of participants, respectively. Antibodies to Wb-Bhp-1 also declined more rapidly after treatment than antibodies to Bm14 in samples from a clinical trial conducted in Sri Lanka. We also tested archived serum samples from people living in filariasis-endemic communities in Egypt with different infection profiles. Antibodies to Wb-Bhp-1 were detected in 73% of microfilaremic people, 53% of amicrofilaremic people with circulating filarial antigen, and 17.5% of endemic individuals without microfilaria or circulating filarial antigen. Tests performed with legacy samples from India showed that few people with filarial lymphedema had antibodies to these recombinant antigens. CONCLUSIONS: Antibodies to Wb-Bhp-1 and Wb123 are more closely correlated with persistent microfilaremia than circulating filarial antigenemia or antibodies to Bm14, and they clear more rapidly after anti-filarial treatment. Additional studies are needed to assess the value of Wb-Bhp-1 serology as a tool for determining the success of LF elimination efforts