To improve multidrug-resistant tuberculosis (MDR-TB) detection, we successfully introduced the rpoB gene mutation line probe assay into the national laboratory in Latvia, a country with epidemic MDR-TB. The assay detected rifampin resistance with 91% sensitivity and 96% specificity within 1 to 5 days (vs. 12–47 days for BACTEC)

Broka, Lonija

Fry, Alicia M.

Greckoseja, Silvija

Holtz, Timothy H.

Leimane, Vaira

Metchock, Beverly

Prokopovica, Inga

Skenders, Girts

Wells, Charles D.

Directory of Open Access Journals

PubMed Central

Emerging Infectious Diseases

English

A clinical, microbiological, and economic analysis of a national service for rapid molecular diagnosis of tuberculosis and rifampin resistance in Mycobacterium tuberculosis.

Comparison of three molecular assays for rapid detection of rifampin resistance in Mycobacterium tuberculosis.

Detection of rifampicin resistance in Mycobacterium tuberculosis isolates from diverse countries by a commercial line probe assay as an initial indicator of multidrug resistance. Int J Tuberc Lung Dis.

Detection of rifampin-resistant Mycobacterium tuberculosis in sputa by nested PCR-linked single-strand conformation polymorphism and DNA sequencing.

Direct detection of multidrug-resistant Mycobacterium tuberculosis in clinical specimens in low- and high-incidence countries by line probe assay.

efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru.

Evaluation of a commercial probe assay for detection of rifampin resistance in Mycobacterium tuberculosis directly from respiratory and nonrespiratory clinical samples. Eur J Clin Microbiol Infect Dis.

Evaluation of the INNO-LiPA Rif. TB assay, a reverse hybridization assay for the simultaneous detection of Mycobacterium tuberculosis complex and its resistance to rifampin. Antimicrob Agents Chemother.

Line probe assay in the rapid detection of rifampinresistant Mycobacterium tuberculosis directly from clinical specimens. Scand J Infect Dis.

Mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis isolates in Spain and their rapid detection by PCRenzyme-linked immunosorbent assay.

Organization. Anti-tuberculosis drug resistance in the world: third global report. Geneva: The Organization;

Public health mycobacteriology, a guide for the level III laboratory. Atlanta: Centers for Disease Control;

Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay. Tuberc Lung Dis.

Rapid detection of smear-negative Mycobacterium tuberculosis by PCR and sequencing for rifampin resistance with DNA extracted directly from slides.

Risk factors for primary multidrug resistant tuberculosis in Latvia, 1999-2001. Int J Tuberc Lung Dis.

Multidrug-resistant Tuberculosis Detection, Latvia