Assessment of the mental health of Irish Adolescents in the community

Aim: This study aims to assess a community of Irish adolescents using the Strengths and Difficulties Questionnaire (SDQ) for behavioural difficulties and mental health disorders. Method: All fifth and sixth class pupils attending eight primary schools were eligible to participate. The self-report version of the SDQ was administered to the pupils in the classroom. Results: Thirty participants (8.7%) had an abnormal SDQ score and 53 (15.3%) had a borderline abnormal SDQ score. Abnormal SDQ scores were more common among females (9.7%; mean score = 11.86; sd = 5.4) than among males (7.6%; mean score = 10.96; sd = 5.26). The difference was most pronounced on the emotional symptoms subscale (females received a mean score of 4.03 [sd = 2.1] compared to a mean male score of 2.90 [sd = 2.1]). Conclusions: Mental health problems are widespread among Irish adolescents. The SDQ is a useful preliminary assessment tool of the mental health profile of Irish adolescents and highlights the need for childhood mental health promotion in schools. The SDQ could also be used in a primary care setting to screen adolescents for mental disorder

Dietary Nitrate Acutely and Markedly Increased Exhaled Nitric Oxide in a Cystic Fibrosis Case

Airway nitric oxide (NO) is a ubiquitous signaling molecule with bronchoprotective, antiinflammatory and anti-infective roles. Cystic fibrosis (CF) is a chronic lung condition associated with deceased exhaled NO. Strategies to increase exhaled NO in CF have yielded inconsistent results. A potential new method of increasing systemic NO involves ingestion of dietary, inorganic nitrate which is reduced to nitrite and NO. We present the case of a 12 year-old, athletic male with CF who demonstrated acute but marked increases in exhaled NO following dietary nitrate consumption compared to placebo

Blunted serum 25(OH)D response to vitamin D3 supplementation in children with autism

Introduction: Data suggest a potential role for vitamin D in autism spectrum disorder (ASD) prevention and treatment. It is likely that the serum response to vitamin D supplementation contributes to its effectiveness. Multiple factors affect serum vitamin D 25(OH)D response to supplementation. Methods: We conducted post-hoc analysis of two double-blind, randomized, placebo-controlled trials (RCT) of vitamin D3 supplementation, one RCT involving children with ASD and another involving children with asthma. Both trials were conducted in the same geographic location (Dublin, Ireland, 53°N), conducted over Winter season and utilized the same vitamin D3 dose (2000 IU/day). Results: We included 18 children with ASD and 17 children with asthma. There was no significant difference in 25(OH)D or age at baseline, however, BMI was significantly lower in ASD (P = 0.03). Compliance with vitamin D supplementation was high in both trials. Despite a significantly longer intervention period (20w vs. 15w; P \u3c 0.0001), ASD children had a significantly lower absolute increase (+26 vs. +45 nmol/l) in 25(OH)D (P = 0.04). Conclusions: Despite similar demographics, children with ASD had a lower increase in 25(OH)D levels with supplementation. Potential mechanisms include altered absorption/metabolism as well as well genetic factors. Clinical and research work relating to vitamin D is ASD should measure 25(OHO)D response to supplementation to assess therapeutic doses

Epigenome-wide Association Studies and the Interpretation of Disease -Omics

Epigenome-wide association studies represent one means of applying genome-wide assays to identify molecular events that could be associated with human phenotypes. The epigenome is especially intriguing as a target for study, as epigenetic regulatory processes are, by definition, heritable from parent to daughter cells and are found to have transcriptional regulatory properties. As such, the epigenome is an attractive candidate for mediating long-term responses to cellular stimuli, such as environmental effects modifying disease risk. Such epigenomic studies represent a broader category of disease -omics, which suffer from multiple problems in design and execution that severely limit their interpretability. Here we define many of the problems with current epigenomic studies and propose solutions that can be applied to allow this and other disease -omics studies to achieve their potential for generating valuable insights

Chaos, Conflict and Distance: A Narrative Analysis of the Experiences of Parents of Children in Inpatient Child and Adolescent Mental Health Services

The prevalence of mental health difficulties among children and adolescents appears to be increasing. In the UK, young people receive treatment for mental health difficulties in child and adolescent mental health services (CAMHS), and those requiring the most intensive treatment receive this in inpatient CAMHS. Research into family caregiving in the context of mental health has favoured spousal relationships and other adult-to-adult relationships. Literature on parents caring for children has concentrated on physical illnesses or disabilities. Research has also favoured objective aspects of caregiver burden, with less attention given to subjective, psychological experiences. A qualitative, systematic review of the experiences of parents caring for children of any age with mental illnesses indicated that no research had explored the experiences of parents who have a child in inpatient CAMHS. As such, the aim of this research was to explore the subjective experiences of parents of children who are receiving treatment in inpatient CAMHS. In-depth interviews were conducted with fifteen parents of children in inpatient CAMHS wards in England. A critical realist ontology was maintained, and the research was conducted within a contextualist epistemology. A narrative analysis was conducted, using Frank’s (1995/2013) narrative types (chaos, restitution, quest) as listening devices. Narratives of restitution and quest were not found to represent the parents’ stories, so two new narrative types were proposed. The three narrative types therefore comprised: narratives of chaos, narratives of conflict, and narratives of distance. These reflected parents’ responses to distressing situations and emotions, and their attempts to make sense of those experiences. The results are critically considered in relation to narrative theory and existing literature on caregiving, and the implications of the research are discussed. The narrative types may be used by clinicians working with parents to better understand their experiences and to tailor therapeutic interventions to support them

Rapid methods of landslide hazard mapping : Fiji case study

A landslide hazard probability map can help planners (1) prepare for, and/or mitigate against, the effects of landsliding on communities and infrastructure, and (2) avoid or minimise the risks associated with new developments. The aims of the project were to establish, by means of studies in a few test areas, a generic method by which remote sensing and data analysis using a geographic information system (GIS) could provide a provisional landslide hazard zonation map. The provision of basic hazard information is an underpinning theme of the UN’s International Decade for Natural Disaster Reduction (IDNDR). It is an essential requirement for disaster preparedness and mitigation planning. This report forms part of BGS project 92/7 (R5554) ‘Rapid assessment of landslip hazards’ Carried out under the ODA/BGS Technology Development and Research Programme as part of the British Government’s provision of aid to developing countries. It provides a detailed technical account of work undertaken in a test area in Viti Levu in collaboration with Fiji Mineral Resources Department. The study represents a demonstration of a methodology that is applicable to many developing countries. The underlying principle is that relationships between past landsliding events, interpreted from remote sensing, and factors such as the geology, relief, soils etc provide the basis for modelling where future landslides are most likely to occur. This is achieved using a GIS by ‘weighting’ each class of each variable (e.g. each lithology ‘class’ of the variable ‘geology’) according to the proportion of landslides occurring within it compared to the regional average. Combinations of variables, produced by summing the weights in individual classes, provide ‘models’ of landslide probability. The approach is empirical but has the advantage of potentially being able to provide regional scale hazard maps over large areas quickly and cheaply; this is unlikely to be achieved using conventional ground-based geotechnical methods. In Fiji, landslides are usually triggered by intense rain storms commonly associated with tropical cyclones. However, the regional distribution of landslides has not been mapped nor is it known how far geology and landscape influence the location and severity of landsliding events. The report discusses the remote sensing and GIS methodology, and describes the results of the pilot study over an area of 713 km2 in south east Viti Levu. The landslide model uses geology, elevation, slope angle, slope aspect, soil type, and forest cover as inputs. The resulting provisional landslide hazard zonation map, divided into high, medium and low zones of landslide hazard probability, suggests that whilst rainfall is the immediate cause, others controls do exert a significant influence. It is recommended that consideration be given in Fiji to implementing the techniques as part of a national strategic plan for landslide hazard zonation mapping

Human rights in a political constitution: an analysis of the compatibility of the Human Rights Act 1998 with political constitutionalism

This thesis presents an analysis of the compatibility of the Human Rights Act 1998 with the theory of political constitutionalism. The theory envisions a constitutional order where those exercising political power are held to account, for the most part via the political process and political institutions. The thesis posits from the position of an observer of the theory that the Act is predominantly compatible with political constitutionalism. Nonetheless, there is scope for reforms which could render a future statutory bill of rights more compatible with political constitutionalism. The first section of the thesis examines the history of political constitutionalism in the United Kingdom (UK) Public Law from its descriptive origins to more recent attempts to construct a normative account. It argues there are actually different conceptions of a normative account and that these accounts might be in conflict with each other over certain issues, even though they are underpinned by a set of shared commitments about the relationship between law and politics and the proper role of political and judicial institutions. The rest of the thesis applies this hypothesis to the Act, analysing the Act’s impact on the UK’s historically political constitution, the compatibility of the Act’s provisions and how proponents of different accounts of political constitutionalism might perceive these changes differently. Finally, the thesis concludes by suggesting how proponents of different accounts of political constitutionalism might suggest different reforms

Optimized design and data analysis of tag-based cytosine methylation assays

Genome-wide, tag-based cytosine methylation analysis is optimized

Genetic recombination is targeted towards gene promoter regions in dogs

The identification of the H3K4 trimethylase, PRDM9, as the gene responsible for recombination hotspot localization has provided considerable insight into the mechanisms by which recombination is initiated in mammals. However, uniquely amongst mammals, canids appear to lack a functional version of PRDM9 and may therefore provide a model for understanding recombination that occurs in the absence of PRDM9, and thus how PRDM9 functions to shape the recombination landscape. We have constructed a fine-scale genetic map from patterns of linkage disequilibrium assessed using high-throughput sequence data from 51 free-ranging dogs, Canis lupus familiaris. While broad-scale properties of recombination appear similar to other mammalian species, our fine-scale estimates indicate that canine highly elevated recombination rates are observed in the vicinity of CpG rich regions including gene promoter regions, but show little association with H3K4 trimethylation marks identified in spermatocytes. By comparison to genomic data from the Andean fox, Lycalopex culpaeus, we show that biased gene conversion is a plausible mechanism by which the high CpG content of the dog genome could have occurred.Comment: Updated version, with significant revision

Epigenomic Modifications Predict Active Promoters and Gene Structure in Toxoplasma gondii

Mechanisms of gene regulation are poorly understood in Apicomplexa, a phylum that encompasses deadly human pathogens like Plasmodium and Toxoplasma. Initial studies suggest that epigenetic phenomena, including histone modifications and chromatin remodeling, have a profound effect upon gene expression and expression of virulence traits. Using the model organism Toxoplasma gondii, we characterized the epigenetic organization and transcription patterns of a contiguous 1% of the T. gondii genome using custom oligonucleotide microarrays. We show that methylation and acetylation of histones H3 and H4 are landmarks of active promoters in T. gondii that allow us to deduce the position and directionality of gene promoters with >95% accuracy. These histone methylation and acetylation “activation” marks are strongly associated with gene expression. We also demonstrate that the pattern of histone H3 arginine methylation distinguishes certain promoters, illustrating the complexity of the histone modification machinery in Toxoplasma. By integrating epigenetic data, gene prediction analysis, and gene expression data from the tachyzoite stage, we illustrate feasibility of creating an epigenomic map of T. gondii tachyzoite gene expression. Further, we illustrate the utility of the epigenomic map to empirically and biologically annotate the genome and show that this approach enables identification of previously unknown genes. Thus, our epigenomics approach provides novel insights into regulation of gene expression in the Apicomplexa. In addition, with its compact genome, genetic tractability, and discrete life cycle stages, T. gondii provides an important new model to study the evolutionarily conserved components of the histone code