Colon cancer cell chemosensitisation by fish oil emulsion involves apoptotic mitochondria pathway

Adjuvant use of safe compounds with anti-tumour properties has been proposed to improve cancer chemotherapy outcome. We aimed to investigate the effects of fish oil emulsion (FOE) rich in n-3 PUFA with the standard chemotherapeutic agents 5-fluorouracil (5-FU), oxaliplatin (OX) or irinotecan (IRI) on two human colorectal adenocarcinoma cells with different genetic backgrounds. The HT-29 (Bax+/+) and LS174T (Bax−/−) cells were co-treated for 24-72h with 1μm-5-FU, 1μm-OX or 10μm-IRI and/or FOE dilution corresponding to 24μm-EPA and 20·5μm-DHA. Soyabean oil emulsion (SOE) was used as isoenergetic and isolipid control. Cell viability, apoptosis and nuclear morphological changes were evaluated by cytotoxic colorimetric assay, flow cytometry analysis with annexin V and 4′,6′-diamidino-2-phenylindole staining, respectively. A cationic fluorescent probe was used to evaluate mitochondrial dysfunction, and protein expression involved in mitochondrial apoptosis was determined by Western blot. In contrast to SOE, co-treatment with FOE enhanced significantly the pro-apoptotic and cytotoxic effects of 5-FU, OX or IRI in HT-29 but not in LS174T cells (two-way ANOVA, P<0·01). These results were confirmed by the formation of apoptotic bodies in HT-29 cells. A significant increase in mitochondrial membrane depolarisation was observed after the combination of 5-FU or IRI with FOE in HT-29 but not in LS174T cells (P<0·05). Co-administration of FOE with the standard agents, 5-FU, OX and IRI, could be a good alternative to increase the efficacy of chemotherapeutic protocols through a Bax-dependent mitochondrial pathwa

Mécanismes de résistance au 5-FU dans le cancer colorectal (implication des récepteurs de TRAIL)

MONTPELLIER-BU Sciences (341722106) / SudocSudocFranceF

Ottimizzazione di un sistema produttivo del settore legno-arredo: il caso Colombini SpA

L'elaborato, che si colloca nel contesto della Lean Manufacturing applicata all'industria del legno-arredo, presenta un caso applicativo di come tale filosofia può essere implementata nelle imprese operanti in questo settore e di quali benefici essa può generare

Indagini biomolecolari per la ricerca di agenti patogeni virali e batterici in campioni di tasso (Meles meles) del Regno Unito

Riassunto In ambito europeo stiamo assistendo alla diffusione e comparsa di malattie emergenti, fenomeno legato alla brusca modificazione della biodiversità da parte dell’uomo. Gli animali selvatici possono svolgere un ruolo fondamentale nella diffusione di malattie infettive (“spreading”) fungendo da serbatoi naturali (“reservoir”) o come possibili diffusori verso specie che sinora non erano venute in contatto con l’agente infettivo (“spillover”). La sorveglianza sanitaria della fauna selvatica, negli ultimi anni, è diventata sempre più oggetto di un crescente interesse, dovuto sia a motivi di salvaguardia della specie che al rischio di diffusione di agenti patogeni di interesse non solo faunistico, ma importanti anche per la sanità pubblica e animale. In questo studio è stata valutata, mediante indagini biomolecolari, la presenza delle principali malattie infettive note o emergenti in una popolazione di tassi del Regno Unito. A tale scopo sono stati analizzati campioni di feci, rene, fegato, milza e sangue da 18 capi messi a disposizione dal Dipartimento di Patologia e Sanità Pubblica dell’Università di Liverpool. Nei campioni fecali la presenza di patogeni quali Parvovirus, Epatite E virus, Reovirus e Astrovirus è stata verificata tramite PCR. I campioni di fegato sono stati analizzati tramite PCR per la ricerca dell’Epatite E virus, Herpes virus, Mycobacterium bovis (M. bovis) e Mycobacterium tuberculosis (M. tuberculosis). I campioni di rene sono stati analizzati con PCR per la ricerca di Leptospira spp. Le indagini biomolecolari effettuate non hanno mostrato positività per Parvovirus, Herpes virus, Epatite E virus, Reovirus, Astrovirus e M. tubercolosis. Tuttavia, la ricerca ha permesso di confermare, in linea con i dati bibliografici, la presenza di Leptospira spp. in due campioni renali e quella di M. bovis in due campioni di fegato. Dato che il campionamento è stato di tipo opportunistico, non è stato possibile ottenere dati di prevalenza. Tuttavia, lo studio e la ricerca continua di malattie infettive note o emergenti nella fauna selvatica costituisce un importante strumento al fine di acquisire informazioni sullo stato sanitario delle popolazioni presenti a livello europeo. ABSTRACT In Europe we are witnessing the spread and the reappearance of emerging diseases. This phenomenon is a result of an abrupt change of biodiversity caused by humans. Wildlife can play a fundamental role in the spread of infectious diseases. Indeed, they can be reservoir or possible spreaders to species that weren’t be in touch with infectious agents (spillover). Over the last few years, animal health surveillance, has gained relevance in public and animal health due to species conservation reasons and the increasing risk for the spreading of pathogens. In this study, the presence of known or emerging infectious diseases in a UK population badgers was assessed by biomolecular studies. For this purpose, samples of feces, kidney, liver, spleen and blood of 18 badgers obtained from Pathology and Public Health Department of University of Liverpool were tested. In feces samples. The presence of Parvovirus, Hepatitis E virus, Reovirus, Astrovirus in feces samples was tested by PCR. The presence of Hepatitis E virus, Herpes virus, Mycobacterium bovis (M. bovis) and Mycobacterium tuberculosis (M. tuberculosis) in liver tissue was also assessed by PCR. The presence of Leptospira ssp. was assessed in kidney samples by PCR. The results indicated that none of the samples resulted positive for Parvovirus, Herpes virus, Hepatitis E virus, Reovirus, Astrovirus e Mycobacterium Tuberculosis. However, accordingly with literature data, this study indicate that two badgers were positive for Leptospira spp. and other two for Mycobacterium bovis. Because the sampling methods was opportunistic, it was not possible to obtain prevalence data. However, the continuous study of wildlife for known or emerging infectious diseases represent an important tool to obtain information about health of wildlife population

« 20 février ». Discours et portraits d’un mouvement de révolte au Maroc

Cet article s’intéresse aux conséquences du « Printemps arabe » dans la seule monarchie du Maghreb. Moins fulgurant que dans d’autres pays, ce « printemps » n’en a pas moins favorisé une réforme constitutionnelle et des élections anticipées. C’est à travers le prisme du « Mouvement du 20 février », plateforme militante ayant réussi à mobiliser plusieurs dizaines de milliers de personnes à travers le Maroc durant le premier semestre 2011 que nous appréhendons les effets concrets de ce « Printemps arabe » in situ et les discours de ses acteurs marocains.This article focuses on the implications of the “Arab Spring” on the only monarchy in the Maghreb. Though less dramatic than in neighboring countries, this “spring” did promote constitutional reform and early elections. We examined the practical effects of this “Arab Spring” on-site and the discourse and its Moroccan players, through the prism of the “February 20 Movement”, an activist platform where tens of thousands of people were mobilized throughout Morocco during the first half of 2011

« 20 février ». Discours et portraits d’un mouvement de révolte au Maroc

Cet article s’intéresse aux conséquences du « Printemps arabe » dans la seule monarchie du Maghreb. Moins fulgurant que dans d’autres pays, ce « printemps » n’en a pas moins favorisé une réforme constitutionnelle et des élections anticipées. C’est à travers le prisme du « Mouvement du 20 février », plateforme militante ayant réussi à mobiliser plusieurs dizaines de milliers de personnes à travers le Maroc durant le premier semestre 2011 que nous appréhendons les effets concrets de ce « Printemps arabe » in situ et les discours de ses acteurs marocains.This article focuses on the implications of the “Arab Spring” on the only monarchy in the Maghreb. Though less dramatic than in neighboring countries, this “spring” did promote constitutional reform and early elections. We examined the practical effects of this “Arab Spring” on-site and the discourse and its Moroccan players, through the prism of the “February 20 Movement”, an activist platform where tens of thousands of people were mobilized throughout Morocco during the first half of 2011