The LHC Logging Service : Handling terabytes of on-line data

Based on previous experience with LEP, a long-term data logging service for the LHC was developed and put in place in 2003, several years before beam operation. The scope of the logging service covers the evolution over time of data acquisitions on accelerator equipment and beam related parameters. The intention is to keep all this time-series data on-line for the lifetime of LHC, allowing easy data comparisons with previous years. The LHC hardware commissioning has used this service extensively prior to the first beams in 2008 and even more so in 2009. Current data writing rates exceed 15TB/year and continue to increase. The high data volumes and throughput rates, in writing as well as in reading, require special arrangements on data organization and data access methods

Counter-current chromatography for the separation of terpenoids: A comprehensive review with respect to the solvent systems employed

Copyright @ 2014 The Authors.This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.Natural products extracts are commonly highly complex mixtures of active compounds and consequently their purification becomes a particularly challenging task. The development of a purification protocol to extract a single active component from the many hundreds that are often present in the mixture is something that can take months or even years to achieve, thus it is important for the natural product chemist to have, at their disposal, a broad range of diverse purification techniques. Counter-current chromatography (CCC) is one such separation technique utilising two immiscible phases, one as the stationary phase (retained in a spinning coil by centrifugal forces) and the second as the mobile phase. The method benefits from a number of advantages when compared with the more traditional liquid-solid separation methods, such as no irreversible adsorption, total recovery of the injected sample, minimal tailing of peaks, low risk of sample denaturation, the ability to accept particulates, and a low solvent consumption. The selection of an appropriate two-phase solvent system is critical to the running of CCC since this is both the mobile and the stationary phase of the system. However, this is also by far the most time consuming aspect of the technique and the one that most inhibits its general take-up. In recent years, numerous natural product purifications have been published using CCC from almost every country across the globe. Many of these papers are devoted to terpenoids-one of the most diverse groups. Naturally occurring terpenoids provide opportunities to discover new drugs but many of them are available at very low levels in nature and a huge number of them still remain unexplored. The collective knowledge on performing successful CCC separations of terpenoids has been gathered and reviewed by the authors, in order to create a comprehensive document that will be of great assistance in performing future purifications. © 2014 The Author(s)

Optimisation of Biochemical Condition and Substrates In Vitro for Tissue Engineering of Ligament

In this work, we analysed the effect of growth factors on in vitro cell proliferation and collagens synthesis by fibroblasts cultured for 72 h on different substrates (silicon sheet with or without 1% gelatin, and glass as control surface) for ligament tissue engineering. A human fibroblast cell line (CRL-2703) was used. The synthesis of type I and type III collagens were evaluated qualitatively and quantitatively by RT-PCR and confocal microscopy, respectively. Cell proliferation was evaluated by two methods: (1) MTT assay (2) cell cycle analysis. It was found that PDGF-AB stimulate the proliferation of fibroblast cultured on gelatin coated silicon sheet in dose dependant manner with a maximum effect at 10 ng ml(−1). The exogenous TGF-β1 induced the expression of type I and type III collagens in a dose and substrate-dependant manner. We deduce from this work that biochemical conditions and substrates have an important impact for optimisation of the tissue neo synthesis

Excrétion urinaire des sulfates : effet du régime

International audienc

STATISTICAL ANALYSIS OF ATOM-PROBE DATA (I) : DERIVATION OF SOME FINE-SCALE FEATURES FROM FREQUENCY DISTRIBUTIONS FOR FINELY DISPERSED SYSTEMS

A statistical model has been developed in order to predict the concentration histograms which are used in atom-probe analyses. This method allows theoretical distributions to be calculated for finely dispersed systems containing spherical particles. Some statistical methods are proposed for the interpretation of experimental histograms

Recalibration of the yellow Rhizocarpon growth curve in the Cordillera Blanca (Peru) and implications for LIA chronology

A new lichen dating method and new moraine observations enabled us to improve the chronology of glacier advances in the Cordillera Blanca (Peru) during the Little Ice Age (LIA). Our results reveal that an early LIA glacial advance occurred around AD 1330 +/- 29. However, a second major glacial advance at the beginning of the 17th century overlapped the earlier stage for most glaciers. Hence, this second glacial stage, dated from AD 1630 +/- 27, is considered as the LIA maximum glacial advance in the Cordillera Blanca. During the 17th-18th centuries, at least three glacial advances were recorded synchronously for the different glaciers (AD 1670 +/- 24, 1730 +/- 21, and 1760 +/- 19). The moraines corresponding to the two first stages are close to the one in 1630 suggesting a slow recession of about 18% in the total length of the glacier. From the LIA maximum extent to the beginning of the 20th century, the 24 glaciers have retreated a distance of about 1000 in, corresponding to a reduction of 30% in their length. This rate is comparable to that observed during the 20th century. Estimates of palaeo-Equilibrium Line Altitudes show an increase in attitude of about 100 m from the LIA maximum glacial extension at the beginning of the 17th century to the beginning of the 20th century. Because long time series are not available for precipitation and temperature, this glacial retreat is difficult to explain by past climate changes. However, there is a fair correspondence between changes in glacier length and the delta O-18 recorded in the Quelccaya ice core at a century timescale. Our current knowledge of tropical glaciers and isotope variations leads us to suggest that this common tropical signal reflects a change from a wet LIA to the drier conditions of today. Finally, a remarkable synchronicity is observed with glacial variations in Bolivia, suggesting a common regional climatic pattern during the LIA