Functional Categories.

<p>Functional Categories.</p

Functional Ambulation Categories.

<p>Functional Ambulation Categories.</p

Descriptive Data Analysis of all 26 individuals.

<p>Descriptive Data Analysis of all 26 individuals.</p

Correlation Analysis at the 2 time points (N = 26).

<p>Correlation Analysis at the 2 time points (N = 26).</p

Network and classification tree analysis of plasma cytokine concentration in non-ATL HTLV-1 infection and ATL.

<p>Network analysis of absolute T-cell subset count, HTLV-1 PVL and plasma cytokine concentration using at least significant Spearman correlation trends is shown for patients with ATL (A), HAM (B) and AC (C). The green and red lines denote positive and negative correlations respectively. The continuous and broken line denote statistically significant and trend correlations. The prune classification tree to classify diagnosis of AC, HAM and ATL on the basis of IL-10 and IL-17 concentration is shown in figure D. The percentage shows the distribution of all patients into different arms whilst the three decimal numbers shown specificity of each classification for diagnosis of AC, ATL and HAM respectively.</p

Plasma cytokine concentration in non-ATL HTLV-1 infection and ATL.

<p>A-I) Aligned column plots of plasma cytokine/chemokine concentrations in patients with non-ATL HTLV-1 infection and ATL. The bar represents median values. The continuous line and shaded area shows manufacturer supplied median and range in healthy individuals. Statistical analysis: Kruskal-Wallis test with Dunn post-test, 95% confidence interval. * denotes p<0.05, ** denotes p<0.01, *** denotes p<0.001.</p

TCR clonality within ‘ATL-like’ cells in patient with non-malignant HTLV-1 infection and ATL.

<p>TCR clonality within ‘ATL-like’ cells in patient with non-malignant HTLV-1 infection and ATL.</p

CD4+CCR4+CD7- T cells as marker of ‘ATL-like’ infected and ATL cells.

<p>Bar column plots showing the absolute frequency and relative of CD4+CCR4+CD7- (A & B) and non CCR4+CD7- CD4+T cells in AC, patients with HAM and ATL showed highly significant increased frequency of CD4+CCR4+CD7- T cells in patients with ATL compared to AC and HAM. The bar represents median values. Statistical analysis: Kruskal-Wallis test with Dunn post-test, 95% confidence interval and Wilcoxon signed rank test. C) XY scatters plots showing significant positive correlation of PVL with relative and absolute frequency of CD4+CCR4+CD7- T cells. The line represents linear regression line with rho and R for Spearman and linear regression correlate respectively. * denotes p<0.05, ** denotes p<0.01, *** denotes p<0.001.</p

Cytokine mRNA expression profile in non-malignant HTLV-1 infection and ATL.

<p>Column plots (A) shows normalized FPKM expression of TNF, IFN, IL-6 and IL-10 linked by patient samples. Statistical analysis: Kruskal-Wallis test with Dunn post-test, 95% confidence interval and Wilcoxon signed rank test. Scatterplot (B) shows correlation between normalized FPKM expression and clonal fraction of two largest clones. Statistical analysis: Spearman correlation. * denotes p<0.05, ** denotes p<0.01, *** denotes p<0.001.</p

Plasma cytokine concentrations in ATL.

<p>A-C) Aligned column plots of plasma cytokine/chemokine concentrations in indolent and aggressive ATL. D) Box plots of fold change in six patients with aggressive ATL at diagnosis and at 3–12 months pre-diagnosis. The bar represents median values. Statistical analysis: Kruskal-Wallis test with Dunn post-test, 95% confidence interval and Wilcoxon signed rank test. * denotes p<0.05, ** denotes p<0.01, *** denotes p<0.001.</p