The Difference in Effect of Arabica Coffee Gayo Beans and Leaf (Coffea Arabica Gayo) Extract on Decreasing Blood Sugar Levels in Healthy Mice

BACKGROUND: High incidence rate of diabetes mellitus (DM) and increased incidence of complications from DM as well as the use of less effective antidiabetic drugs and high financing to treat demands alternative therapy. AIM: This study was conducted to determine the difference in the effect of Arabica coffee gayo bean and leaf extract on blood sugar levels in healthy mice before and after the intervention of extract and fasting and postprandial blood glucose level after consumption of glucose. METHODS: This research is an experimental research study. The study used experimental animals which were divided into 8 groups which are the control group (Aquadest), group given Acarbose, the group given the Arabica coffee gayo bean extract with dosage of 50 mg, 100 mg and 200 mg in healthy mice, the group given the Arabica coffee gayo leaf extract with dosage of 30 mg, 60 mg and 120 mg in healthy mice. RESULTS: The results of the study obtained are before and after the intervention of extract showed the difference of p= 0.523 and after consumption of glucose the fasting and postprandial result showed the difference of p = 0.005. CONCLUSION: The use of Arabica coffee gayo bean and leaf extract showed a high decrease in blood glucose levels (BGL) compared to the control group

SETD2 loss-of-function promotes renal cancer branched evolution through replication stress and impaired DNA repair

The research leading to these results is supported by Cancer Research UK (XYG, RAB, EG, PM, PE, SG, C Santos, AJR, NM, PAB, AS and C Swanton), Breast Cancer Research Foundation (C Swanton and NK), Medical Research Council (ID: G0902275 to MG and C Santos; ID: G0701935/2 to AJR and C Swanton), the Danish Cancer Society (AMM, J Bartkova and J Bartek), the Lundbeck Foundation (R93-A8990 to J Bartek), the Ministry of the interior of the Czech Republic (grant VG20102014001 to MM and J Bartek), the National Program of Sustainability (grant LO1304 to MM and J Bartek), the Danish Council for Independent Research (grant DFF-1331-00262 to J Bartek), NIHR RMH/ICR Biomedical Research Centre for Cancer (JL), the EC Framework 7 (PREDICT 259303 to XYG, EG, PM, MG, TJ and C Swanton; DDResponse 259892 to J Bartek and J Bartkova and RESPONSIFY ID:259303 to C Swanton), UCL Overseas Research Scholarship (SG). C Swanton is also supported by the European Research Council, Rosetrees Trust and The Prostate Cancer Foundation. This research is supported by the National Institute for Health Research University College London Hospitals Biomedical Research Centre

Antiplatelet Effectivity between Aspirin with Honey on Cardiovascular Disease Based on Bleeding Time Taken on Mice

BACKGROUND: The prevalence of cardiovascular disease (CVD) and its mortality continues to increase. Various studies have shown aspirin can reduce CVD mortality but has adverse side effects. Research on a comparison between aspirin and honey has not been done, but both have antiplatelet effects. AIM: This study is aimed to prove the antiplatelet effects on honey and compare the antiplatelet effects of aspirin with honey based on the bleeding time in mice. METHODS: This study is a true experimental design with a post-test only control group using 32 male mice, Double Ditsch Webster, ± 3 months old, the weight of 20-30 g, divided into 4 groups. Consisting of a negative control group (placebo), aspirin and honey. The suspension has given orally for 12 days using the probe. The research was conducted at the Laboratory of Pharmacology Department of Pharmacology and Therapeutics Faculty of Medicine, the University of North Sumatra in September until December 2015. The data collected was bleeding time in mice. Data analysed by Shapiro Wilk test, Kruskal Wallis and Mann Whitney. RESULTS: The mean bleeding time was a placebo (102.88 seconds), aspirin (369.38 seconds) and honey (304.63 seconds). Mann Whitney test showed significant results in the aspirin and honey groups against the control group (placebo) with p = 0.001. There were no significant differences in the aspirin group against honey (p = 0.172). Honey has an antiplatelet effect in mice. The mean bleeding time in mice given honey is longer or closer to the mean bleeding time in the aspirin group. CONCLUSION: The results could be used as a basis for further research to determine its use in humans with cardiovascular disease

The Effectiveness of Acetone Breath Content Using Chitosan Based Sensor in Patients Diabetes Mellitus

BACKGROUND: The high incidence of diabetes mellitus in Indonesia to encourage researchers to continue to develop detection techniques are easy, inexpensive and minimally invasive. This study is expected to answer the challenge. Detection tool used in this research is the detection devices use traditional breath acetone levels of chitosan-based sensors. Acetone breath sensor works based on the chemical interaction between the breath of patients with diabetes and acetone sensor surface, which causes a change in the electrical response. AIM: This study was cross-sectional using diabetes mellitus patients as research subjects by measuring breath acetone levels and HbA1c and KGD as a comparison. METHODS: Research conducted at the primary health care facilities network services the University Hospital of North Sumatra. A total of 58 diabetic patients who meet the inclusion and exclusion criteria were included in this study. The relationship between the measurements analysed using Pearson correlation test. RESULTS: Measurements showed that statistically there is a great relationship between breath acetone levels with or KGD HbA1c levels in the diabetic patient with each R = 0.05 and p = 0.708 for the relationship acetone breath and HbA1c and R = 0.1 and p = 0.445 for the relationship acetone breath and KGD. However, clinically there is a linear relationship between increased levels consistent acetone in the breath with increased HbA1c levels as well as levels of KGD. CONCLUSION: Examination of breath acetone levels can be considered as an indicator of diabetes detection