Phosphorus Fertilizer and Stocking Rate Effects on Soil Microbial Biomass of a Long-Term Dairy Farmlet Experiment

The effects of a range of P fertilizer rates and dairy cow stocking rates on microbial biomass carbon and phosphorus were compared in a long-term farmlet trial in southeastern Australia. Pastures were stocked at 2, 3, or 4 cows/ha and received fertilizer at rates of 0, 35, 70 or 140 kgP/ha. There was no effect of either P fertilizer rate or stocking rate on microbial biomass C from 1995 to 1998. Increasing P application rate significantly increased the chloroform-released microbial P flush measured, but stocking rate had no effect on microbial P. There were significant temporal changes, with the seasonal effects of soil temperature and moisture overriding treatment effects on these microbial measurements

Responses to Nitrogen Fertilizer in Dairy Pastures with Differing Phosphorus Fertility in South Eastern Australia

The application of nitrogen (N) fertilizer to temperate pastures is an increasingly popular management tool for boosting pasture production on dairy farms in Australia. However, limited information is available about N fertilizer responses that can be obtained from pastures with varying levels of phosphorus (P) fertility. A field experiment examining the initial and residual response of pasture growth to urea was carried out within a large dairy farmlet study, in October 1998. The four P fertility treatments (Olsen P values) ranged from 9 to 32 mg/kg. Dry matter yields increased with increasing N rates in all treatments, at both harvests. Only pastures with Olsen P values of \u3c 12 mg/kg had a significantly lower response to N fertilizer

Grazing Management Impacts on the Riparian Zone and Water Quality

Inappropriate farm management activities such as stock access to creeks, and poor fertiliser and effluent management can negatively impact riparian zones and waterways, contributing to increased in-stream nutrient, sediment and microbiological loads and loss of riparian biodiversity, amongst other impacts. Nutrient budgets for dairy systems indicate that on-farm nutrient accumulation and redistribution is common (Gourley 2004), which in large part is due to the uneven distribution of dairy cow dung and the nutrients they contain (Aarons et al., 2004). The \u27Gippsland Dairy Riparian Project Environmental Monitoring module\u27 was established in Jan. 2003 to monitor the impact of dairy farm management and changed riparian zone management on the riparian zone and water quality

Fertiliser Responses and Soil Test Calibrations for Grazed Pastures in Australia

On-farm management of fertiliser is of major economic significance to the Australian grazing industries, based on expenditure on fertiliser and higher farm productivity that fertiliser use supports. However the application of fertiliser has traditionally been an inexact and inefficient process (Peverill et al. 1999) and there is increasing pressure for nutrient losses from agriculture to be minimised. The improved adoption and application of tools like soil testing can make substantial improvements in nutrient use efficiency but interpretation needs to be based on the best available information. This paper reports on the collation of current and historical experimental data relating to pasture production - fertiliser response relationships (nitrogen, phosphorus, potassium and sulphur) for various pasture types, climatic zones and soils across Australia

Contribution of Dairy Cow Manure to Soil Fertility and Nutrient Redistribution in Pastures

The effects of dairy cow manure on soil fertility were investigated at the site of the long-term phosphorus rate by stocking rate farmlet experiment at the Dairy Research Institute, Ellinbank, in Victoria. Manure increased extractable soil P (Olsen) in the 0 - 5 cm layer after 60 days to 61 mg/kg compared with values of 32 mg/kg in the control soils. Extractable soil K (Colwell) almost doubled under manure pads to 5 cm depth from 642 mg/kg in control soils to 1226 mg/kg in manure treated soils. The effects of grazing management on nutrient redistribution and pasture growth within strip-grazed paddocks was also investigated. While soil Olsen P was not different, Colwell K (p \u3c 0.001) and pasture height (p \u3c 0.01) were significantly greater at the front or gate-end of the paddocks compared with the back

Phosphorus Fertilizer Requirements for Temperate Dairy Pastures and Milk Production in South Eastern Australia

Phosphorus (P) fertilizer is required to sustain productive pastures in Australia, yet optimum P fertility is poorly defined. A large farmlet study involving 10 herds was established in 1995 to determine the relationship between milk production and pasture response to four different P fertilizer rates, at three stocking rates. Pasture growth significantly increased with increasing P fertilizer rate. Cumulative milk production from pasture over 4 years, was significantly affected by P fertilizer, but responses diminished with increasing P rate. The results from this study will enable a better targeting of P fertility levels for profitable milk production from temperate dairy pastures and minimise the environmental impact of excess P fertilizer use

Fertiliser Responses and Soil Test Calibrations for Grazed Pastures in Australia

On-farm management of fertiliser is of major economic significance to the Australian grazing industries, based on expenditure on fertiliser and higher farm productivity that fertiliser use supports. However the application of fertiliser has traditionally been an inexact and inefficient process (Peverill et al. 1999) and there is increasing pressure for nutrient losses from agriculture to be minimised. The improved adoption and application of tools like soil testing can make substantial improvements in nutrient use efficiency but interpretation needs to be based on the best available information. This paper reports on the collation of current and historical experimental data relating to pasture production - fertiliser response relationships (nitrogen, phosphorus, potassium and sulphur) for various pasture types, climatic zones and soils across Australia

Heterogeneous Nutrient Distribution Across Dairy Grazing Systems in Southeastern Australia

The Australian dairy industry is largely based on a grazed pasture system, although most cows also consume substantial amounts of imported feed (Fulkerson & Doyle 2001). This trend is expected to increase as the Australian dairy industry continues to intensify. Fertiliser inputs of nitrogen (N), phosphorus (P), potassium (K) and sulphur (S) are still viewed as necessary to maintain adequate pasture and milk production despite the fact that most dairy farms are in net positive balance for all of these nutrients (Reuter 2001). Nutrient losses from dairy farming regions and eutrophication of waterways has gained strong public and political attention and intensive pasture systems are no longer seen as ‘clean and green’. An important aspect of a viable dairy industry in the future will be more refined nutrient management planning

FANCD2 expression affects platinum response and further characteristics of high grade serous ovarian cancer in cells with different genetic backgrounds

High-grade serous ovarian cancer (HGSOC) is the most prevalent subtype of ovarian cancer and demonstrates 5-year survival of just 40%. One of the major causes of mortality is the development of tumour resistance to platinum-based chemotherapy, which can be modulated by dysregulation of DNA damage repair pathways. We therefore investigated the contribution of the DNA interstrand crosslink repair protein FANCD2 to chemosensitivity in HGSOC. Increased FANCD2 protein expression was observed in some cell line models of platinum resistant HGSOC compared with paired platinum sensitive models. Knockdown of FANCD2 in some cell lines, including the platinum resistant PEO4, led to increased carboplatin sensitivity. Investigation into mechanisms of FANCD2 regulation showed that increased FANCD2 expression in platinum resistant cells coincides with increased expression of mTOR. Treatment with mTOR inhibitors resulted in FANCD2 depletion, suggesting that mTOR can mediate platinum sensitivity via regulation of FANCD2. Tumours from a cohort of HGSOC patients showed varied nuclear and cytoplasmic FANCD2 expression, however this was not significantly associated with clinical characteristics. Knockout of FANCD2 was associated with increased cell migration, which may represent a non-canonical function of cytoplasmic FANCD2. We conclude that upregulation of FANCD2, possibly mediated by mTOR, is a potential mechanism of chemoresistance in HGSOC and modulation of FANCD2 expression can influence platinum sensitivity and other tumour cell characteristics

RFWD3 modulates response to platinum chemotherapy and promotes cancer associated phenotypes in high grade serous ovarian cancer

Background: DNA damage repair is frequently dysregulated in high grade serous ovarian cancer (HGSOC), which can lead to changes in chemosensitivity and other phenotypic differences in tumours. RFWD3, a key component of multiple DNA repair and maintenance pathways, was investigated to characterise its impact in HGSOC.Methods: RFWD3 expression and association with clinical features was assessed using in silico analysis in the TCGA HGSOC dataset, and in a further cohort of HGSOC tumours stained for RFWD3 using immunohistochemistry. RFWD3 expression was modulated in cell lines using siRNA and CRISPR/cas9 gene editing, and cells were characterised using cytotoxicity and proliferation assays, flow cytometry, and live cell microscopy. Results: Expression of RFWD3 RNA and protein varied in HGSOCs. In cell lines, reduction of RFWD3 expression led to increased sensitivity to interstrand crosslinking (ICL) inducing agents mitomycin C and carboplatin. RFWD3 also demonstrated further functionality outside its role in DNA damage repair, with RFWD3 deficient cells displaying cell cycle dysregulation, reduced cellular proliferation and reduced migration. In tumours, low RFWD3 expression was associated with increased tumour mutational burden, and complete response to platinum chemotherapy. Conclusions: RFWD3 expression varies in HGSOCs, which can lead to functional effects at both the cellular and tumour levels.<br/