Punicic Acid a Conjugated Linolenic Acid Inhibits TNFα-Induced Neutrophil Hyperactivation and Protects from Experimental Colon Inflammation in Rats

BACKGROUND:Neutrophils play a major role in inflammation by releasing large amounts of ROS produced by NADPH-oxidase and myeloperoxidase (MPO). The proinflammatory cytokine TNFalpha primes ROS production through phosphorylation of the NADPH-oxidase subunit p47phox on Ser345. Conventional anti-inflammatory therapies remain partially successful and may have side effects. Therefore, regulation of neutrophil activation by natural dietary components represents an alternative therapeutic strategy in inflammatory diseases such as inflammatory bowel diseases. The aim of this study was to assess the effect of punicic acid, a conjugated linolenic fatty acid from pomegranate seed oil on TNFalpha-induced neutrophil hyperactivation in vitro and on colon inflammation in vivo. METHODOLOGY AND PRINCIPAL FINDINGS:We analyzed the effect of punicic acid on TNFalpha-induced neutrophil upregulation of ROS production in vitro and on TNBS-induced rat colon inflammation. Results show that punicic acid inhibited TNFalpha-induced priming of ROS production in vitro while preserving formyl-methionyl-leucyl-phenylalanine (fMLP)-induced response. This effect was mediated by the inhibition of Ser345-p47phox phosphorylation and upstream kinase p38MAPK. Punicic acid also inhibited fMLP- and TNFalpha+fMLP-induced MPO extracellular release from neutrophils. In vivo experiments showed that punicic acid and pomegranate seed oil intake decreased neutrophil-activation and ROS/MPO-mediated tissue damage as measured by F2-isoprostane release and protected rats from TNBS-induced colon inflammation. CONCLUSIONS/SIGNIFICANCE:These data show that punicic acid exerts a potent anti-inflammatory effect through inhibition of TNFalpha-induced priming of NADPH oxidase by targeting the p38MAPKinase/Ser345-p47phox-axis and MPO release. This natural dietary compound may provide a novel alternative therapeutic strategy in inflammatory diseases such as inflammatory bowel diseases

QUASAR QUAlité des Services informatiques pour les AsR

Le groupe QUASAR ( pour " Qualité des Services informatiques pour les AsR ") a été créé en 2012 dans le cadre du CCRI. Il compte actuellement une dizaine de personnes. Au cours de cet exposé, le groupe présentera ses objectifs ainsi que ses premiers travaux, en particulier les résultats de l'enquête "Etat des lieux de la qualité dans les services informatique à l'IN2P3" menée en septembre 2012 auprès des laboratoires

LAPP site report - A French Tier3

TransparentsLAPP (Laboratoire d'Annecy le Vieux de Physique des Particules) is a French IN2P3 laboratory involved in LHC experiments (Atlas, LHCb) as a Tier3. We will describe our computing resources (shared by local and grid users), storage resources, services running at site, monitoring and “home made” accounting tools

Backup system migration at LAPP : from a local infrastructure to IN2P3 centralized system

Lapp is one of the French IN2P3 labs located in Annecy-le-Vieux. In this presentation, we will explain how we moved our tape backup system from a local infrastructure to a centralized one. We were using Networker (Legato) software for more than ten years with a small LTO2 library and we switched to a mutualized infrastructure powered by CC IN2P3 using Tivoli (IBM) software. This talk will describe the new infrastructure (based on information provided by our CC-IN2P3 colleagues) and then we will detail the different steps for this migration including the integration with GPFS. We will point out the difficulties we met, the impacts on our organization and infrastructure and our future plans. On the other hand, we will also report the CC-IN2P3 point of view as a service provider