Evidencia serológica de infección por metapneumovirus en aves comerciales de la provincia de Buenos Aires y Entre Ríos (Argentina)

A serological investigation was made in commercial flocks of Buenos Aires and Entre Rios provinces by ELISA technique. The serological evidence of metapneumovirus infection was determined by testing 933 serum samples from broilers flocks and broiler-breeders between March of 2007 and October of 2008. 169 of these samples were positives. The result of this study was analyzed by statistics methods. The conclusion shows a better possibility to obtain positive results in broiler-breeders than in broilers flocks. This is the first report of avian metapneumovirus serological evidence in the Argentinian Republic.Se realizo un relevamiento serológico en aves comerciales de las provincias de Buenos Aires y Entre Ríos mediante la técnica de ELISA. La evidencia serológica de la infección con metapneumovirus se determino procesando 933 sueros provenientes de pollos parrilleros y gallinas reproductoras, desde el mes de marzo del año 2007 al mes de octubre del año 2008, de los cuales 169 fueron positivos. Estos resultados fueron analizados mediante métodos estadísticos arribando a la conclusión de que existe mayor probabilidad de obtener aves seropositivas en la categoría de reproductores que en la de pollos parrilleros. Así mismo se demuestra por primera vez la evidencia de serología positiva contra metapneumovirus de la República Argentina

Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis

Adult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolated from the liver, spleen, heart, lungs, kidneys and intestines from each of the five animals evaluated. The five strains were susceptible to ampicillin, cephalotin, cefotaxime, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, nitrofurantoin and trimethoprimsulfamethoxazole, and resistant to tetracycline. Each of the five S. Typhimurium isolates was analyzed by XbaI- pulsed-field gel electrophoresis (PFGE), showing an identical electrophoretic profile with 15 defined bands, which was found to be identical to pattern ARJPXX01.0220 of the PulseNet Argentine National database of Salmonella PFGE patterns. This is the first work describing the postmortem diagnosis of an outbreak of salmonellosis in chinchillas by using molecular methods such as PFGE