The Aetiology of Congenital Deformities Due to Disproportional Growth During Development of the Foetus

Abstract Not Provided

Genes Associated With Prognosis After Surgery For Malignant Pleural Mesothelioma Promote Tumor Cell Survival In Vitro

<p>Abstract</p> <p>Background</p> <p>Mesothelioma is an aggressive neoplasm with few effective treatments, one being cytoreductive surgery. We previously described a test, based on differential expression levels of four genes, to predict clinical outcome in prospectively consented mesothelioma patients after surgery. In this study, we determined whether any of these four genes could be linked to a cancer relevant phenotype.</p> <p>Methods</p> <p>We conducted a high-throughput RNA inhibition screen to knockdown gene expression levels of the four genes comprising the test (ARHGDIA, COBLL1, PKM2, TM4SF1) in both a human lung-derived normal and a tumor cell line using three different small inhibitory RNA molecules per gene. Successful knockdown was confirmed using quantitative RT-PCR. Detection of statistically significant changes in apoptosis and mitosis was performed using immunological assays and quantified using video-assisted microscopy at a single time-point. Changes in nuclear shape, size, and numbers were used to provide additional support of initial findings. Each experiment was conducted in triplicate. Specificity was assured by requiring that at least 2 different siRNAs produced the observed change in each cell line/time-point/gene/assay combination.</p> <p>Results</p> <p>Knockdown of ARHGDIA, COBLL1, and TM4SF1 resulted in 2- to 4-fold increased levels of apoptosis in normal cells (ARHGDIA only) and tumor cells (all three genes). No statistically significant changes were observed in apoptosis after knockdown of PKM2 or for mitosis after knockdown of any gene.</p> <p>Conclusions</p> <p>We provide evidence that ARHGDIA, COBLL1, and TM4SF1 are negative regulators of apoptosis in cultured tumor cells. These genes, and their related intracellular signaling pathways, may represent potential therapeutic targets in mesothelioma.</p

What works in investigative interviewing? Using Systematic Maps to examine the evidence base

Competent investigative interviewing skills are key to securing reliable information from victims, witnesses, informants, and suspects. Information obtained in interviews often plays an important role in directing an investigation, informing effective decision-making, promoting efficient allocation of resources, as well as securing reliable prosecutions and mitigating risk of miscarriages of justice. However, effective investigative interviewing is a complex skill to master; demanding a sound understanding of the many cognitive, social, and environmental factors that influence the content and accuracy of witness and suspect accounts. To ensure that investigative interviewing and intelligence gathering produces usable, credible, and reliable information in an effective and ethically defensible manner, training and practice must be evidence-based. This short article outlines how practitioners, trainers and policy makers can navigate the best available research evidence to evaluate ‘what works?’ in investigative interviewing

Recent Results from the Lunar Reconnaissance Orbiter Mission and Plans for the Extended Mission

The Lunar Reconnaissance Orbiter spacecraft (LRO), launched on June 18, 2009, began with the goal of seeking safe landing sites for future robotic missions or the return of humans to the Moon as part of NASA's Exploration Systems Mission Directorate (ESMD). In addition, LRO's objectives included the search for surface resources and to investigate the Lunar radiation environment. After spacecraft commissioning, this phase of the mission began on September 15, 2009, completed on September 15, 2010 when operational responsibility for LRO was transferred to NASA's Science Mission Directorate (SMD). The SMD mission is scheduled for 2 years and will be completed in 2012 with an opportunity for an extended mission beyond 2012. Under SMD, the mission focuses on a new set of goals related to understanding the geologic history of the Moon, its current state, and what it can tell us about the evolution of the Solar System. Having marked the two year anniversary will review here the major results from the LRO mission for both exploration and science and discuss plans and objectives going forward including a proposed 2-year extended mission. These objectives include: 1) understanding the bombardment history of the Moon, 2) interpreting Lunar geologic processes, 3) mapping the global Lunar regolith, 4) identifying volatiles on the Moon, and 5) measuring the Lunar atmosphere and radiation environment

Hot Rocks: Constraining the Thermal Conditions of the Mistastin Lake Impact Melt Deposits Using Zircon Grain Microstructures

The production of superheated melt during hypervelocity impact events has been proposed to be a common occurrence on terrestrial planetary bodies. Recent direct evidence of superheated impact melt temperatures exceeding \u3e2370°C from the Kamestastin (Mistastin Lake) impact structure, Canada, was based on a single impact glass sample. Such high superheated melt temperatures have strong implications for the evolution of crustal material, the thermal history of impact cratering events, and the rheology of impact melt. However, although widely predicted in previous studies, with the exception of the Mistastin Lake impact glass, there is little direct evidence for superheated temperatures in multiple settings across an impact structure. Therefore, an outstanding question is how heterogeneous are superheated conditions across a single impact structure. In this work, we analyze the crystallographic orientations and microstructures of zircon grains and the precursor parent phases of baddeleyite crystals, from four different samples representing the entire melt-bearing stratigraphy at Mistastin: an impact glass, a vesicular clast-poor impact melt rock, a clast-rich impact melt rock, and a glass-bearing impact breccia. Using electron microprobe analysis followed by electron backscatter diffraction, we discovered that four zircon grains with vermicular coronae of baddeleyite crystals from the impact glass contain evidence for a cubic zirconia precursor, indicative of temperature conditions \u3e2370°C. We also report evidence of superheating up to 1673°C in the glass-bearing impact breccia. In addition, we also report the first occurrence at Mistastin of the high-pressure zircon polymorph reidite and former reidite in granular neoblastic (FRIGN) zircon in grains from the glass-bearing impact breccia, implying minimum peak shocks from 30–40 GPa. The identification of superheating from two localities at Mistastin demonstrates (1) that superheating is not restricted solely to rapidly cooled impact melt rock samples and is therefore more distributed across impact structures, and (2) we can investigate the P-T evolution pathways of impact melt from different impact settings, providing a clearer picture of the thermal conditions and history of the impact structure

Archaeological Excavations at Nethermills Farm, Deeside, 1978-81

This publication is grant aided by Historic Environment Scotland.Peer reviewedPublisher PD

Interpretations of Lava Flow Properties from Radar Remote Sensing Data

The surface morphology and roughness of a lava flow provides insight on its lava properties and emplacement processes. This is essential information for understanding the eruption history of lava fields, and magmatic processes beneath the surface of Earth and other planetary bodies such as the Moon. The surface morphology is influenced by lava properties such as viscosity, temperature, composition, and rate of shear. In this work, we seek to understand how we can interpret the emplacement processes and lava properties of lava flows using remote sensing data. Craters of the Moon (COTM) National Monument and Preserve in Idaho hosts a suite of compositionally diverse lava flows with a wide range of surface roughness making it the ideal case study. Lava flows there have surface morphologies consistent with smooth pāhoehoe, slabby pāhoehoe, hummocky pāhoehoe, rubbly pāhoehoe, ‘a’ā, block-`a’ā, and blocky textures. The variation in surface roughness across the lava field reflects changes in lava properties and/or emplacement processes over space and time. We investigate geochemical and petrographic variations of the different lava flow morphologies and analyse how they relate to airborne radar data. Results show L-Band (24 cm) radar circular polarization ratios (CPR) distinguish the contrasting surface roughness at COTM, separating the smoother (primitive; low SiO2 and alkali) and rougher (evolved; high SiO2 and alkali) lava flows. However, ambiguities are present when comparing the CPR values for rubbly pāhoehoe and block-`a’ā flow. Even though their CPR values appear similar at the decimetre scale, they have distinct morphologies that formed under different emplacement processes. Without ground-truth information, the rubbly pāhoehoe and block-`a’ā lava flows could therefore be misinterpreted to be the same type of flow morphology, which would lead to false interpretations about their lava properties and emplacement processes. This is important when comparing these flows to lava flows on other planetary bodies that share similar CPR values, such as the Moon. Thus, using terrestrial analogues such as those at COTM can provide an improved understanding of the surface morphology and emplacement processes of lunar lava flows. This will lead to more refined interpretations about past volcanic processes on the Moon

Differentiating Fissure-Fed Lava Flow Types and Facies Using RADAR and LiDAR: An Example from the 2014–2015 Holuhraun Lava Flow-field

Distinguishing between lava types and facies using remote sensing data is important for interpreting the emplacement history of lava flow-fields on Earth and other planetary bodies. Lava facies typically include a mixture of lava types and record the collective emplacement history of material preserved at a particular location. We seek to determine if lava facies in the 2014–2015 Holuhraun lava flow-field are discernible using radar roughness analysis. Furthermore, we also seek to distinguish between lava types using high resolution Light Detection and Ranging (LiDAR) data. We extracted circular polarization ratios (CPR) from the Uninhabited Aerial Vehicle Synthetic Aperture Radar and cross-polarization (VH/VV) data from the Sentinel-1 satellite to analyze the surface roughness of three previously mapped lava facies: rubbly, spiny, and undifferentiated rubbly–spiny. Using the Kruskal-Wallis test, we reveal that all but one pair of the facies are statistically separable. However, the populations overlap by 88%–89% for CPR and 64%–67% for VH/VV. Therefore, owing to large sample populations (n \u3e 2 × 105), slight differences in radar data may be used to probabilistically infer the presence of a particular facies, but not directly map them. We also calculated the root-mean-square slope and Hurst exponents of five different lava types using LiDAR topography (5 cm/pixel). Our results show minute differences between most of the lava types, with the exception of the rubbly pāhoehoe, which is discernible at 1σ. In brief, the presence of “transitional” lava types (e.g., rubbly pāhoehoe) within fissure-fed lava flow-fields complicates remote sensing-based mapping