Preliminary study on the use of near infrared hyperspectral imaging for quantitation and localisation of total glucosinolates in freeze-dried broccoli

peer-reviewedThe use of hyperspectral imaging to (a) quantify and (b) localise total glucosinolates in florets of a single broccoli species has been examined. Two different spectral regions (vis–NIR and NIR), a number of spectral pre-treatments and different mask development strategies were studied to develop the quantitative models. These models were then applied to freeze-dried slices of broccoli to identify regions within individual florets which were rich in glucosinolates. The procedure demonstrates potential for the quantitative screening and localisation of total glucosinolates in broccoli using the 950–1650 nm wavelength range. These compounds were mainly located in the external part of florets.Universidad de SevillaJ.M. Hernández-Hierro thanks the Spanish MICINN for the Juan de la Cierva contract (JCI-2011-09201) and Universidad de Sevilla for the mobility Grant (Universidad de Sevilla Research Plan). Spanish MICINN Project AGL2011-30254-C02 and Junta de Andalucia PGC Project AGR 6331