3 research outputs found
Test immuchromatographique rapide pour le diagnostic sérologique de la tuberculose
The lCT tuberculosis is an in vitro immunodiagnostic test for the detection of
antibodies to Mycobacterium tuberculosis in serum or plasma. This test targets 5
recombinant M tuberculosis proteins, including the 38 and 85 KDa antigens. We
evaluated this test on 65 sera from patients with and without TB living in Liege.
Patients and methods: This test was performed on two groups : The TB group included
31 patients condidered to have an active tuberculosis : pulmonary (26 cases) or extrapulmonary (5 cases). All of them had at least one sample with positive culture and
received anti-tuberculous treatment. The identification of TB strains was performed
with Accuprobe M tuberculosis complexe (Gen-Probe). The second group (control
group) included 34 patients with the following characteristics: BCG negative nonexposed
healthy (19), BCG positive or exposed healthy (2), non-TB respiratory
disease (10), atypical TB (3). The identification of the atypical mycobacteria was
determined according to the biochemical characteristics or by molecular biology.
Results. The sensitivity is 38.7%, the specificity is 94%. The positive predictive value is 85.7%
and the negative predictive value is 62,7%. Two false lCT positive results were found
in the control group: both patients had a past infection with TB.
Conclusion: This test is very easy and rapid to perform, has a good specificity but
lacks sensitivity in low prevalence countries. A positive lCT allows same day
diagnosis of Mycobacterium tuberculosis infection