Understanding the functions of kinesin-II

AbstractSpecies ranging from Chlamydomonas to humans possess the heterotrimeric kinesin-II holoenzyme composed of two different motor subunits and one non-motor accessory subunit. An important function of kinesin-II is that it transports the components needed for the construction and maintenance of cilia and flagella from the site of synthesis in the cell body to the site of growth at the distal tip. Recent work suggests that kinesin-II does not directly interact with these components, but rather via a large protein complex, which has been termed a raft (intraflagellar transport (IFT)). While ciliary transport is the best-established function for kinesin-II, evidence has been reported for possible roles in neuronal transport, melanosome transport, the secretory pathway and during mitosis

Unidirectional inline skates

Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2010.Cataloged from PDF version of thesis.Includes bibliographical references.In this thesis, I describe the design, manufacture, assembly, and testing of "1WSkates," a pair of inline skates that prevent backwards motion. The defining element of 1WSkates is the use of a one-way bearing which, along with a fixed axle, prevents certain wheels from rolling backwards. The axle is custom-shaped to lock into an existing inline skate housing. The one-way bearing is attached to the wheel with a custom fitting. The complete 1WSkates allow a user to walk up an incline and roll down the other side, and to use a nordic skiing motion on flat terrain.by Adam J. Goldstein.S.B

Clustering-Based Materialized View Selection in Data Warehouses

Materialized view selection is a non-trivial task. Hence, its complexity must be reduced. A judicious choice of views must be cost-driven and influenced by the workload experienced by the system. In this paper, we propose a framework for materialized view selection that exploits a data mining technique (clustering), in order to determine clusters of similar queries. We also propose a view merging algorithm that builds a set of candidate views, as well as a greedy process for selecting a set of views to materialize. This selection is based on cost models that evaluate the cost of accessing data using views and the cost of storing these views. To validate our strategy, we executed a workload of decision-support queries on a test data warehouse, with and without using our strategy. Our experimental results demonstrate its efficiency, even when storage space is limited

Sunday Driver links axonal transport to damage signaling

Neurons transmit long-range biochemical signals between cell bodies and distant axonal sites or termini. To test the hypothesis that signaling molecules are hitchhikers on axonal vesicles, we focused on the c-Jun NH2-terminal kinase (JNK) scaffolding protein Sunday Driver (syd), which has been proposed to link the molecular motor protein kinesin-1 to axonal vesicles. We found that syd and JNK3 are present on vesicular structures in axons, are transported in both the anterograde and retrograde axonal transport pathways, and interact with kinesin-I and the dynactin complex. Nerve injury induces local activation of JNK, primarily within axons, and activated JNK and syd are then transported primarily retrogradely. In axons, syd and activated JNK colocalize with p150Glued, a subunit of the dynactin complex, and with dynein. Finally, we found that injury induces an enhanced interaction between syd and dynactin. Thus, a mobile axonal JNK–syd complex may generate a transport-dependent axonal damage surveillance system

Mitochondrial Association of a Plus End–Directed Microtubule Motor Expressed during Mitosis in Drosophila

The kinesin superfamily is a large group of proteins (kinesin-like proteins [KLPs]) that share sequence similarity with the microtubule (MT) motor kinesin. Several members of this superfamily have been implicated in various stages of mitosis and meiosis. Here we report our studies on KLP67A of Drosophila. DNA sequence analysis of KLP67A predicts an MT motor protein with an amino-terminal motor domain. To prove this directly, KLP67A expressed in Escherichia coli was shown in an in vitro motility assay to move MTs in the plus direction. We also report expression analyses at both the mRNA and protein level, which implicate KLP67A in the localization of mitochondria in undifferentiated cell types. In situ hybridization studies of the KLP67A mRNA during embryogenesis and larval central nervous system development indicate a proliferation-specific expression pattern. Furthermore, when affinity-purified anti-KLP67A antisera are used to stain blastoderm embryos, mitochondria in the region of the spindle asters are labeled. These data suggest that KLP67A is a mitotic motor of Drosophila that may have the unique role of positioning mitochondria near the spindle

Pre-Hawking Radiation from a Collapsing Shell

We investigate the effect of induced massive radiation given off during the time of collapse of a massive spherically symmetric domain wall in the context of the functional Schr\"odinger formalism. Here we find that the introduction of mass suppresses the occupation number in the infrared regime of the induced radiation during the collapse. The suppression factor is found to be given by $e^{-\beta m}$, which is in agreement with the expected Planckian distribution of induced radiation. Thus a massive collapsing domain wall will radiate mostly (if not exclusively) massless scalar fields, making it difficult for the domain wall to shed any global quantum numbers and evaporate before the horizon is formed.Comment: 10 pages, 3 figures. We updated the acknowledgments as well as added a statement clarifying that we are following the methods first laid out in Phys. Rev. D 76, 024005 (2007