294 research outputs found

    N-terminal cysteine bioconjugation with (2-cyanamidophenyl)boronic acids enables the direct formation of benzodiazaborines on peptides

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    Benzodiazaborines (BDABs) have emerged as a valuable tool to produce stable and functional bioconjugates via a click-type transformation. However, the current available methods to install them on peptides lack bioorthogonality, limiting their applications. Here, we report a strategy to install BDABs directly on peptide chains using (2-cyanamidophenyl)boronic acids (2CyPBAs). The resulting BDAB is stabilized through the formation of a key intramolecular B–N bond. This technology was applied in the selective modification of N-terminal cysteine-containing functional peptides

    Morphological responses, fruit yield, nutritive value and in vitro gas production of forage watermelon genotypes on semi-arid condition.

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    This study aimed to evaluate morphological, bromatological, in vitro gas production and yield of forage watermelon (Citrullus lanatus var. citroides) genotypes in semi-arid condition. Seven genotypes were evaluated were BGCIA 228, BGCIA 239, Jojoba, BGCIA 228 x BGCIA239, BGCIA 228 x BGCIA Jojoba, BGCIA 239 x Jojoba and BGCIA 991. The experimental design was a complete randomized block with three replicates. The genotypes presented differences between the characteristics: fruit length (P = 0.01), vertical diameter (P = 0.02), peel thickness (P = 0.01), fruit pulp thickness (P = 0.02), transversal diameter (P = 0.02), in vitro dry matter digestibility (P = 0.003) and the latency time (P < 0.0001). Cumulative in vitro gas production and gas production rate was not affected by genotypes. None of the studied genotypes had production and productivity affected. Among them, Jojoba and BGCIA 991 stood out for having heavier and longer fruits, and a higher peel thickness and pulp length

    Atividades de quitinase após eliciação das defesas da soja contra a ferrugem asiática.

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    Para avaliar a influência do jasmonato de metila (JM) sobre a atividade da quitinase (proteína relacionada à patogênese), assim como, o potencial do eliciador na redução do progresso da ferrugem asiática, no estádio V5 as plantas da cultivar suscetível BRS 361 foram pulverizadas com água, Tween 20 a 0,02 % e JM a 1,25 mM. Após 24 horas da pulverização, as plantas foram inoculadas com 1,4x105 uredósporos/mL de Phakopsora pachyrhizi; como controle foi aplicado água. Utilizou-se delineamento em blocos ao acaso, com seis blocos, cada vaso contendo cinco plantas. Coletaram-se folhas V4 e V5, às 48, 96, 144 e 192 horas após a inoculação. Folhas de plantas não inoculadas foram coletadas nos mesmos períodos. A atividade da quitinase foi determinada pelo método proposto por Robert & Selitrennikoff (Journal of General Microbiology, v. 134, n. 1, p. 169-176, 1988), modificado por Harman et al. (Phytopathology, v. 83, n. 3, p. 313-318, 1993). A atividade da quitinase foi superior em folhas de soja inoculadas em comparação com as não inoculadas, independente dos produtos aplicados. A aplicação de JM aumentou a atividade da quitinase em folhas submetidas ou não a inoculação, independente do período de avaliação. A aplicação de Tween 20 também aumentou a atividade em comparação com a água, independente da inoculação, embora com intensidade sempre menor que o JM. Os resultados obtidos sugerem que a inoculação com ferrugem asiática da soja induz a atividade da quitinase e que eliciadores JM e Tween 20 podem ser usados para avaliar a resistência da soja ao patógeno

    New emigration and Portuguese society: Transnationalism and return

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    This chapter addresses the theme of transnationalism and return in recent Portuguese emigration, namely the flows that occurred after the turn of the century. It starts with a brief theoretical overview on those topics, which constitute two relatively neglected characteristics of Portuguese emigration. Next, based on a survey carried out in 2014–2015 to more than 6000 recent emigrants, it reveals some of the links that they maintain with their home country, as well as their plans for the future, which include settlement in the destination country, return and re-emigration. Lastly, it examines data on returning emigrants – especially those that returned between 2001 and 2011 – extracted from the 2011 Census. The evidence reveals a significant number of returns, including individuals at both working and retirement ages and at all skill levels, thus exposing the unexpected complexity of movements. The results are based on the research project “Back to the future: new emigration and links with Portuguese society” (REMIGR), which aimed to ascertain the extent and characteristics of the new emigration wave. The project included an overview of emigration and return to and from all regions of the world, as well as case studies in UK, France, Luxembourg, Angola, Mozambique and Brazil.info:eu-repo/semantics/publishedVersio

    Resistência induzida em genótipos de soja infestados por Bemisia tabaci biótipo B.

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    O presente estudo objetivou identificar e quantificar as substâncias químicas encontradas em genótipos de soja infestados e não infestados por B. tabaci e que possam estar relacionadas à defesa da planta
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