Flagellin Delivery by Pseudomonas aeruginosa Rhamnolipids Induces the Antimicrobial Protein Psoriasin in Human Skin

The opportunistic pathogen Pseudomonas aeruginosa can cause severe infections in patients suffering from disruption or disorder of the skin barrier as in burns, chronic wounds, and after surgery. On healthy skin P. aeruginosa causes rarely infections. To gain insight into the interaction of the ubiquitous bacterium P. aeruginosa and healthy human skin, the induction of the antimicrobial protein psoriasin by P. aeruginosa grown on an ex vivo skin model was analyzed. We show that presence of the P. aeruginosa derived biosurfactant rhamnolipid was indispensable for flagellin-induced psoriasin expression in human skin, contrary to in vitro conditions. The importance of the bacterial virulence factor flagellin as the major inducing factor of psoriasin expression in skin was demonstrated by use of a flagellin-deficient mutant. Rhamnolipid mediated shuttle across the outer skin barrier was not restricted to flagellin since rhamnolipids enable psoriasin expression by the cytokines IL-17 and IL-22 after topical application on human skin. Rhamnolipid production was detected for several clinical strains and the formation of vesicles was observed under skin physiological conditions. In conclusion we demonstrate herein that rhamnolipids enable the induction of the antimicrobial protein psoriasin by flagellin in human skin without direct contact of bacteria and responding cells. Hereby, human skin might control the microflora to prevent colonization of unwanted microbes in the earliest steps before potential pathogens can develop strategies to subvert the immune response

Tracer gas technique, air velocity measurement and natural ventilation method for estimating ventilation rates through naturally ventilated barns

Naturally ventilated barns have the advantage of providing an energy-efficient and low-noise micro-environment for animals.  Such barns are very common, especially for cattle, which are a major source of ammonia and methane emissions as well as other airborne pollutants. Emission fluxes are calculated as the product of the ventilation rate (VR) and the gas concentration.  The VR of naturally ventilated barns are directly influenced by atmospheric conditions.  Therefore, the estimation of VR involves high uncertainties; furthermore, there is no reference method for VR estimation.  For these reasons, three different methods were investigated to estimate VR through naturally ventilated dairy barns: (1) the tracer gas technique, using radioactive isotope Krypton-85 (85Kr), (2) the measurement of the air velocity through the barn openings, and (3) the natural ventilation method.  The investigations were performed in two naturally ventilated dairy barns (each with a volume of 5,670 m3) during mild (T≥17℃) and cold weather (T<17℃) conditions.  The VR ranged between 48,000 and 475,000 m3/h.  There was a significant (p<0.01) correlation between all three methods for barn A (Pearson’s correlation coefficient 0.59-0.86).  For barn B only, the tracer gas and the natural ventilation method showed a significant (p=0.03) correlation (Pearson’s correlation coefficient 0.62).  The season (mild or cold) and the VR estimation method both had a significant (p<0.03) effect on the estimated VR for both barns.  The outside wind speed had a significant (p<0.005) effect on the estimated VR for barn A.  For barn B, this effect was not significant (p=0.052).  The tracer gas technique used has the advantage of measuring the tracer with high resolution in time (1 Hz) and space (18 or more measured points).Keywords: Natural ventilation, tracer gas technique, radioactive isotope 85Kr, air velocity, air exchange rate, dairy barn  &nbsp

The p.Arg435His Variation of IgG3 With High Affinity to FcRn Is Associated With Susceptibility for Pemphigus Vulgaris—Analysis of Four Different Ethnic Cohorts

IgG3 is the IgG subclass with the strongest effector functions among all four IgG subclasses and the highest degree of allelic variability among all constant immunoglobulin genes. Due to its genetic position, IgG3 is often the first isotype an antibody switches to before IgG1 or IgG4. Compared with the other IgG subclasses, it has a reduced half-life which is probably connected to a decreased affinity to the neonatal Fc receptor (FcRn). However, a few allelic variants harbor an amino acid replacement of His435 to Arg that reverts the half-life of the resulting IgG3 to the same level as the other IgG subclasses. Because of its functional impact, we hypothesized that the p.Arg435His variation could be associated with susceptibility to autoantibody-mediated diseases like pemphigus vulgaris (PV) and bullous pemphigoid (BP). Using a set of samples from German, Turkish, Egyptian, and Iranian patients and controls, we were able to demonstrate a genetic association of the p.Arg435His variation with PV risk, but not with BP risk. Our results suggest a hitherto unknown role for the function of IgG3 in the pathogenesis of PV

Effects of vibrations and shocks on lithium-ion cells

Lithium-ion batteries are increasingly used in mobile applications where mechanical vibrations and shocks are a constant companion. This work shows how these mechanical loads affect lithium-ion cells. Therefore pouch and cylindrical cells are stressed with vibrational and shock profiles according to the UN 38.3 standard. Additionally, a vibration test is set up to reflect stress in real-world applications and is carried out for 186 days. The effects of the load profiles on the tested cells are investigated by capacity measurement, impedance spectroscopy, micro-X-ray computed tomography and post mortem analyses. The mechanical stress has no effect on the investigated pouch cells. Although all tested cylindrical cells would pass the standard tests, in certain cells stressed in a vertical position the mandrel dispatched itself and struck against internal components. This caused bruised active materials, short circuits, a damaged current collector and current interrupt device. The investigations are not directly transferrable to all pouch or cylindrical cells but show that the mechanical cell design, especially the fixation of the internal components, determines whether a cell withstands vibrations and shocks. Depending on the cell design and the loading direction, long-term vibrational loads can have additional detrimental effects on lithium-ion cells compared to standard tests