La valutazione della genitorialità. una ricerca esplorativa, per capire come venga vissuta da un gruppo di cittadini romani

This paper reports the data of an exploratory research conducted with a group of Roman citizens who were asked what they thought about the possibility of assessing the parenting of families considered at risk over the parental expertise. Citizens were interviewed and the interviews were submitted to AET, Emotional Analysis of the Text. Assessing parenting often involves families with internal conflicts. We think the conflict is not only there. Conflicts also cross the theoretical, legal, and political hypotheses, which base the opportunity to intervene within the issues involved in parental evaluation. They are also found in the relationship between families and services. Legal and sociological literature advise the presence of these conflicts and discuss the issues; whereas the psychological and psychiatric one is focused on the application of techniques, whether they are evaluation, psychotherapy, re-education. This perspective does not consider the conflicting complexity which founds the parental evaluation, but it is focused only on the family. We were interested in analysing, in this context, what is the citizens’ perception of the evaluation of parenting, who are their potential customers and users; in particular, if and how these conflictual dynamics are perceived by them. After the analysis of the interview, the data outline three cultures. One proposes the traditional Italian family, the feminised family and designated to the care of members in difficulty within the home wall; family today in a strong crisis within processes of change. Another proposes the centrality of the court and the legal culture, founded on evaluations and resolving actions that often can result in child custody actions. These two cultures contrast: when the traditional family is missing, the court appears. There is no counseling which concerns the changes of the family. Psychological and neuropsychiatric counseling agencies do not appear in the data, except for social services, in any case subordinated to the court. A third culture proposes an “ideal” adult, who with balance and reflection skills, has to face a fearful context, but it is an isolated individual, without a relational context of reference. This culture seems to opposite the dissolution of relational contexts – state, organizations, family – to the idealized individual, self-determined and alone. The development of psychological function in the assessment of parenting concerns two aspects: the importance of studying the complexity of the social mandate and the intervention with the conflicting relationships of the family and of all the other actors involved

Preliminary Validation of the CI-FRA Checklist: A Simple Screening Tool for Measuring the Early Signs of Reading and Spelling Disorders in Italian Primary Students

Although several screening tests for recognizing early signs of reading and spelling difficulties have been developed, brief and methodologically grounded tools for teachers are very limited. The present study aimed to lay the foundation for a new screening tool for teachers: the Checklist for early Indicators of risk Factors in Reading Ability (CI-FRA). The proposed checklist consists of 20 items, based on a 7-point Likert scale, and it investigates five domains: reading, writing, attention, and motor skills. Six hundred sixtyseven children were evaluated by 40 teachers during the first year of primary school and, longitudinally, in the second year. Exploratory factor analysis and confirmatory factor analysis (CFA) were applied to verify structural validity. Concurrent validity was assessed by Spearman correlation to analyze the link between CI-FRA and reading and spelling standardized tests and cognitive tests. Reliability was assessed by Cronbach a and interclass correlation coefficient. The CFA reported a three-factor structure as the optimal solution, including language (reading and writing), visuospatial attention, and fine motor skills subscales. Good reliability, good internal consistency, and acceptable test\u2013 retest indices were found. Concurrent validity was confirmed by significant correlations between CI-FRA total score and standardized reading and spelling test, as well as by correlations between CI-FRA subscales and neuropsychological standardized test scores. Preliminary evaluation of sensitivity by receiver operating characteristic curves showed that the CI-FRA score has particularly high sensitivity and specificity for word reading speed deficit. In conclusion, the results confirm that CI-FRA is a theoretically grounded and statistically valid tool that could help the teachers to screen for early signs of reading and spelling difficulties

PMCA-based detection of prions in the olfactory mucosa of patients with Sporadic Creutzfeldt-Jakob Disease

Sporadic Creutzfeldt-Jakob disease (sCJD) is a rare neurodegenerative disorder caused by the conformational conversion of the prion protein (PrPC) into an abnormally folded form, named prion (or PrPSc). The combination of the polymorphism at codon 129 of the PrP gene (coding either methionine or valine) with the biochemical feature of the proteinase-K resistant PrP (generating either PrPSc type 1 or 2) gives rise to different PrPSc strains, which cause variable phenotypes of sCJD. The definitive diagnosis of sCJD and its classification can be achieved only post-mortem after PrPSc identification and characterization in the brain. By exploiting the Real-Time Quaking-Induced Conversion (RT-QuIC) assay, traces of PrPSc were found in the olfactory mucosa (OM) of sCJD patients, thus demonstrating that PrPSc is not confined to the brain. Here, we have optimized another technique, named protein misfolding cyclic amplification (PMCA) for detecting PrPSc in OM samples of sCJD patients. OM samples were collected from 27 sCJD and 2 genetic CJD patients (E200K). Samples from 34 patients with other neurodegenerative disorders were included as controls. Brains were collected from 26 sCJD patients and 16 of them underwent OM collection. Brain and OM samples were subjected to PMCA using the brains of transgenic mice expressing human PrPC with methionine at codon 129 as reaction substrates. The amplified products were analyzed by Western blot after proteinase K digestion. Quantitative PMCA was performed to estimate PrPSc concentration in OM. PMCA enabled the detection of prions in OM samples with 79.3% sensitivity and 100% specificity. Except for a few cases, a predominant type 1 PrPSc was generated, regardless of the tissues analyzed. Notably, all amplified PrPSc were less resistant to PK compared to the original strain. In conclusion, although the optimized PMCA did not consent to recognize sCJD subtypes from the analysis of OM collected from living patients, it enabled us to estimate for the first time the amount of prions accumulating in this biological tissue. Further assay optimizations are needed to faithfully amplify peripheral prions whose recognition could lead to a better diagnosis and selection of patients for future clinical trials

Isolation and characterization of extracellular vesicles secreted by pre-pubertal Sertoli cells

Recent studies have shown that extracellular vesicles (Ev) are an important mechanism of intercellular communication. In fact, Ev may contain proteins, DNA and mRNA. In particular, the latter play an important role in various biological processes including regulation and cell differentiation [1]. Sertoli cells (SC), previously considered as a mere “sustentacular cell”, were re-evalued in their functions and elevated to the rank of a “sentinel” in spermatogenesis due to production of trophic, differentiation and immune-modulators factors. Porcine pre-pubertal SC, isolated by our method [2], upon 48 hours culture, SC were stimulated with recombinant a-follitropin (rFSH) or FSH + testosterone (T) to evaluate both the presence in the medium of SC-derived Ev (SC-Ev) and SC-Ev content, in terms of mRNA for Anti-Müllerian hormone (AMH), inhibin B, Androgen Binding Protein (ABP) and FSH-receptor (FSH-r), by RT-PCR. SEM analysis highlighted the presence of SC-Nv in culture medium with mean diameters < 149 nm. We have also demonstrated, within the SC-Ev, significant increase in mRNA for AMH, ABP and FSH-r after both FSH and FSH+T stimulation, as compared to unstimulated SC-Ev. Differently from unstimulated SC-Ev, mRNA inhibin B levels were unchanged in FSH-stimulated SC-Ev, and increased after FSH+T stimulation. Interestingly, an opposite trend was shown in mRNA secretion, in control SC monolayer where, we demonstrated a decrease of AMH and FSH-r mRNA (after both stimulations with FSH or FSH + T) and an increase of inhibin B mRNA. On the contrary, mRNA ABP levels, in SC monolayer, decreased after stimulation with FSH but were unchanged in the presence of FSH+T. For the first time in the Literature, our work has shown the presence of SC-Nv containing AMH, inhibin B, ABP and FSH-r mRNA regulated by FSH with or without T. This result may suggest that other testicular cells could produce factors that, until now, were considered an exclusive SC secretory product.This work was supported by Mr.Gary Harlem (Altucell Inc. 3 Astor Court, Dix Hills, New York, NY) and Merck-Serono (London, UK)

I Feel what You Feel if You Are Similar to Me

Social interactions are influenced by the perception of others as similar or dissimilar to the self. Such judgements could depend on physical and semantic characteristics, such as membership in an ethnic or political group. In the present study we tested whether social representations of the self and of others could affect the perception of touch. To this aim, we assessed tactile perception on the face when subjects observed a face being touched by fingers. In different conditions we manipulated the identity of the shown face. In a first experiment, Caucasian and Maghrebian participants viewed a face belonging either to their own or to a different ethnic group; in a second experiment, Liberal and Conservative politically active participants viewed faces of politicians belonging to their own or to the opposite political party. The results showed that viewing a touched face most strongly enhanced the perception of touch on the observer's face when the observed face belonged to his/her own ethnic or political group

I Feel What You Feel if I Like You: The Effect of Attractiveness on Visual Remapping of Touch

Observing touch being applied to another human's face enhances tactile perception for touch being applied to one's own face. This effect, termed the Visual Remapping of Touch (VRT), is maximal the greater the physical or conceptual similarity between observer and observed. An interesting possibility, however, is that even though the basic nature of the VRT is multisensory, a high cognitive level affinity from the observer toward the observed could modulate the VRT even in the face of decreased physical similarity. In the present study we manipulate the level of attractiveness of the avatars that participants observed being touched. By doing so, we either increased (attractive) or decreased (unattractive) the interpersonal judgment value toward the avatar, while always decreasing the physical semblance between the avatar shown and the original image. Results revealed that both for an avatar depicting oneself or a stranger, the VRT is present when touch is applied to an attractive, but not to an unattractive avatar. These findings suggest that basic multisensory effects, such as visuo-tactile interaction, are modulated by higher-level cognitive representations of the self and of others

Sport e disturbi del neurosviluppo Campus: l'organizzazione del lavoro Che cosa non fare e consigli per situazioni frequenti: tabelle per soluzioni pratiche APPENDICE A Scheda osservazionale

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Visual enhancement of touch depends on the Political similarity between the observed and observer's face.

<p>Panel A: Tactile perception was enhanced when observers belonging to Democratic and Conservative parties viewed the face of a member of their own political party (In-group) rather than of the opposite (Out-group) political party. Panel B reports data from Conservative and Democratic observers plotted separately.</p

Experimental design.

<p>Subjects performed the tactile confrontation task in a two by two design. Over a series of trials they viewed either In-group or Out-group faces, with those groups defined by either ethnic (Experiment 1) or political (Experiment 2) similarity to the viewer. The depicted face could be touched (Touch Condition) or simply approached (No-Touch condition) by one or two fingers on either the right, left or both sides of the depicted face. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004930#pone-0004930-g001" target="_blank">Figure 1</a> shows examples of stimuli from Experiment 2: the face of a Democratic (on the left) and a Conservative (on the right) political leader, was either touched (on the top) or simply approached (on the bottom) by two fingers. The pictures on the left represent an In-group condition for a Democratic observer and an Out-group condition for a Conservative observer and vice-versa for the pictures on the right.</p

Visual enhancement of touch depends on the Ethnic similarity between the observed and observer's face.

<p>Panel A: Tactile perception (measured as accuracy in detecting bilateral tactile stimuli) was enhanced when observers from Caucasian and Maghrebian ethnic groups viewed a face from their own ethnic group (In-group condition; left column) rather than a face from the other ethnic group (Out-group); the effect was specific to the observation of touch (Touch condition, Black bars). Panel B reports data from Caucasian and Maghrebian observers plotted separately.</p