Chromosome-Specific Single-Locus FISH Probes Allow Anchorage of an 1800-Marker Integrated Radiation-Hybrid/Linkage Map of the Domestic Dog Genome to All Chromosomes

We present here the first fully integrated, comprehensive map of the canine genome, incorporating detailed cytogenetic, radiation hybrid (RH), and meiotic information. We have mapped a collection of 266 chromosome-specific cosmid clones, each containing a microsatellite marker, to all 38 canine autosomes by fluorescence in situ hybridization (FISH). A 1500-marker RH map, comprising 1078 microsatellites, 320 dog gene markers, and 102 chromosome-specific markers, has been constructed using the RHDF5000-2 whole-genome radiation hybrid panel. Meiotic linkage analysis was performed, with at least one microsatellite marker from each dog autosome on a panel of reference families, allowing one meiotic linkage group to be anchored to all 38 dog autosomes. We present a karyotype in which each chromosome is identified by one meiotic linkage group and one or more RH groups. This updated integrated map, containing a total of 1800 markers, covers >90% of the dog genome. Positional selection of anchor clones enabled us, for the first time, to orientate nearly all of the integrated groups on each chromosome and to evaluate the extent of individual chromosome coverage in the integrated genome map. Finally, the inclusion of 320 dog genes into this integrated map enhances existing comparative mapping data between human and dog, and the 1000 mapped microsatellite markers constitute an invaluable tool with which to perform genome scanning studies on pedigrees of interest

Cross-Sector Collaboration, Institutional Gaps, and Fragility: The Role of Social Innovation Partnerships in a Conflict-Affected Region

The authors aim to contribute to the literature on subsistence marketplaces and the marketing field in general by exploring social innovation partnerships in a fragile country characterized by institutional gaps—specifically, by considering the role of cross-sector collaboration in conflict-affected areas. The empirical setting consists of coffee partnerships in the Democratic Republic of the Congo, where the authors collected data from and about companies, nongovernmental organizations, and cooperatives using both primary and secondary sources, including a field trip, interviews, and group discussions with farmers and their families. They show results at the organizational level (i.e., buildup of managerial capacities, transfer of financial-administrative skills, and improved functioning of cooperatives), the farmer level (i.e., better prices, livelihoods, and access to markets as well as increased revenues), and the community level (i.e., reduced tensions and collaboration between previously hostile groups as well as the creation of new governance modalities). The study suggests that partnerships may offer a systemic approach to addressing institutional gaps, which is necessary in such “extreme” contexts. The authors close with a discussion of further implications for research and public policy