2 research outputs found
Primjena lanÄane reakcije polimerazom za odreÄivanje genetski modificiranog zrnja soje sorte Roundup ReadyTM u smjesi s drugim sortama
Strong increase in the production of genetically modified organisms (GMOs) observed over the years has led to a consolidation of transgenic seed industries worldwide. The dichotomy between the evaluated risk and the perceived risk of transgenic use has defined their level of acceptability among different global societies. GMOs have been widely applied to agricultural commodities, among them the Roundup Readyā¢ (RRā¢) soybean line GTS 40-3-2 has become the most prevalent transgenic crop in the world. This variety was developed to confer plant tolerance against glyphosate-based agricultural herbicide Roundup Readyā¢. Issues related to detection and traceability of GMOs have gained worldwide interest due to their increasing global diffusion and the related socioeconomic and health implications. Also, due to the widespread use of GMOs in food production, labelling regulations have been established in some countries to protect the right of consumers and producers. Besides
regulatory demand, consumer concern issues have resulted in the development of several methods of detecting and quantifying foods derived from genetically engineered crops and their raw materials. Polymerase chain reaction (PCR) has been proven to be the method of choice to detect the presence or absence of the introduced genes of GMOs at DNA level. The present paper aims to verify whether the PCR technique can detect RRā¢ soybean seeds among conventional ones to further certification as non-GM soybean seeds and grains. This analysis could be accomplished through the development of new methodology called \u27intentional contamination\u27 of soybean conventional seeds or grains with the respective RRā¢ soybeans. The results show that the PCR method can be applied with high sensitivity in order to certify conventional soybean seeds and grains.PoveÄana je proizvodnja genetski modificiranog organizama uzrokovala konsolidaciju industrije transgenog sjemena diljem svijeta. Dihotomija izmeÄu procijenjenog i opaženog rizika uporabe transgenog sjemena odredila je razinu njegove prihvatljivosti u razliÄitim druÅ”tvenim zajednicama. Genetski se modificirani organizmi veÄ uvelike primjenjuju u poljoprivredi, a Roundup ReadyTM (RRTM) linija soje GTS 40-3-2 prevladavajuÄi je transgeni usjev u svijetu. Ova je sorta razvijena da bi stekla otpornost na glifosatni herbicid Roundup ReadyTM. OdreÄivanje i sljedivost genetski modificiranih organizama (GMO) vrlo je važno jer se GMO sve viÅ”e koristi, a njegova primjena ima socioekonomske i zdravstvene posljedice. TakoÄer se zbog raÅ”irene uporabe GMO u proizvodnji hrane u nekim zemljama uvela obveza oznaÄavanja proizvoda radi zaÅ”tite prava proizvoÄaÄa i potroÅ”aÄa. Osim zahtjeva za regulativom, potroÅ”aÄi traže i nadzor nad GMO, pa je razvijeno nekoliko metoda za detekciju i kvantificiranje hrane koja potjeÄe iz transgenih usjeva i sirovina. LanÄana reakcija polimerazom (PCR) najbolja je metoda za detekciju introduciranih gena GMO na razini DNK. Stoga je u radu istraženo može li PCR metoda odrediti zrna RRTM soje meÄu konvencionalnim zrnjem, te može li se primijeniti za certifikaciju sjemena koje nije genetski modificirano. Postupak se sastoji od namjerne kontaminacije konvencionalnog sjemena zrnjem RRTM soje i odreÄivanja GMO zrnja PCR metodom. UtvrÄeno je da se ova metoda s visokom sigurnoÅ”Äu može primijeniti pri certifikaciji konvencionalnog sjemena
Application of Polymerase Chain Reaction for High Sensitivity Detection of Roundup Readyā¢ Soybean Seeds and Grains in Varietal Mixtures
Strong increase in the production of genetically modified organisms (GMOs) observed over the years has led to a consolidation of transgenic seed industries worldwide. The dichotomy between the evaluated risk and the perceived risk of transgenic use has defined their level of acceptability among different global societies. GMOs have been widely applied to agricultural commodities, among them the Roundup Readyā¢ (RRā¢) soybean line GTS 40-3-2 has become the most prevalent transgenic crop in the world. This variety was developed to confer plant tolerance against glyphosate-based agricultural herbicide Roundup Readyā¢. Issues related to detection and traceability of GMOs have gained worldwide interest due to their increasing global diffusion and the related socioeconomic and health implications. Also, due to the widespread use of GMOs in food production, labelling regulations have been established in some countries to protect the right of consumers and producers. Besides
regulatory demand, consumer concern issues have resulted in the development of several methods of detecting and quantifying foods derived from genetically engineered crops and their raw materials. Polymerase chain reaction (PCR) has been proven to be the method of choice to detect the presence or absence of the introduced genes of GMOs at DNA level. The present paper aims to verify whether the PCR technique can detect RRā¢ soybean seeds among conventional ones to further certification as non-GM soybean seeds and grains. This analysis could be accomplished through the development of new methodology called 'intentional contamination' of soybean conventional seeds or grains with the respective RRā¢ soybeans. The results show that the PCR method can be applied with high sensitivity in order to certify conventional soybean seeds and grains