Post-weaning growth of cavies (Cavia porcellus) according to the inclusion level of ginger (Zingiber officinale) meal in the diet as a feed additive

The use of phytobiotics in feeding monogastric herbivores has several interests, including the stabilization of the caecal flora and especially the improvement of the growth of animals thanks to a better exploitation of feed resources. Thus a trial was conducted with an objective to evaluate the feed intake and post weaning growth performances of cavy according to the inclusion level of ginger meal. A total of 40 guinea pigs of 3 weeks of age were divided into 4 lots, and subjected to rations consisting of Trypsacum laxum served ad libitum, associated with the compound feed containing 0; 0.5; 0.75 and 1% ginger powder (T0, T1, T2 and T3). This study showed that the total intake of DM, MO and CB from forage was comparable between rations T0, T1 and T2 and significantly higher than those of ration T3. At weaning, average animal weights of all rations were comparable. However, at 8 weeks of age, the T0, T1 and T3 rations yielded comparable average weights, but significantly higher than those of the T2 animals, i.e., 272 g; 298.80 g; 285.25 g and 221.83 g, respectively. The T0, T1 and T3 diets recorded total gains that were comparable but significantly higher than those obtained with animals in Lot T2. Ginger powder can be included up to 0.75% in the diet as a feed additive for improved ingestion and 0.5% for post-weaning growth. L’usage des phytobiotiques dans l’alimentation des herbivores monogastriques présente plusieurs intérêts parmi lesquels la stabilisation de la flore caecale et surtout l’amélioration de la croissance des animaux grâce à une meilleure valorisation des ressources alimentaire. C’est ainsi que des essais ont été conduit avec pour objectif d’évaluer l’ingestion er la croissance post-sevrage des cobayes en fonction du niveau de la poudre de gingembre. Un total de 40 cobayes de 21 jours d’âge était reparti en 4 lots, et soumis à des rations constituées de Trypsacum laxum servi ad libitum, associé à l’aliment composé contenant 0 ; 0,5 ; 0,75 et 1% de poudre de gingembre (T0, T1, T2 et T3). Il ressort de cette étude que les ingestions totales de la MS, de la MO et de la CB du fourrage, ont été comparables entre les rations T0, T1 et T2 et significativement plus élevées que celle de la ration T3. Au sevrage, les poids moyens des animaux étaient comparables pour toutes les rations. Cependant à 8 semaines d’âge, les rations T0, T1 et T3 ont permis d’obtenir les poids moyens comparables, mais significativement plus élevés que ceux des animaux lot T2, soit respectivement 272 g ; 298,80 g ; 285,25 g et 221,83 g. Les rations T0, T1 et T3 ont permis d’enregistrer les gains totaux comparables, mais significativement élevés par rapport à ceux obtenus avec les animaux du lot T2. La poudre de gingembre peut être incluse jusqu’à 0,75% dans la ration comme additif alimentaire pour l'amélioration de l’ingestion et à 0,5% pour la croissance post-sevrage

Comparing two service delivery models for the prevention of mother-to-child transmission (PMTCT) of HIV during transition from single-dose nevirapine to multi-drug antiretroviral regimens

<p>Abstract</p> <p>Background</p> <p>Mother-to-child transmission (MTCT) of HIV has been eliminated from the developed world with the introduction of multi-drug antiretroviral (md-ARV) regimens for the prevention of MTCT (PMTCT); but remains the major cause of HIV infection among sub-Saharan African children. This study compares two service delivery models of PMTCT interventions and documents the lessons learned and the challenges encountered during the transition from single-dose nevirapine (sd-nvp) to md-ARV regimens in a resource-limited setting.</p> <p>Methods</p> <p>Program data collected from 32 clinical sites was used to describe trends and compare the performance (uptake of HIV testing, CD4 screening and ARV regimens initiated during pregnancy) of sites providing PMTCT as a stand-alone service (<it>stand-alone site</it>) versus sites providing PMTCT as well as antiretroviral therapy (ART) (<it>full package site</it>). CD4 cell count screening, enrolment into ART services and the initiation of md-ARV regimens during pregnancy, including dual (zidovudine [AZT] +sd-nvp) prophylaxis and highly active antiretroviral therapy (HAART) were analysed.</p> <p>Results</p> <p>From July 2006 to December 2008, 1,622 pregnant women tested HIV positive (HIV+) during antenatal care (ANC). CD4 cell count screening during pregnancy increased from 60% to 70%, and the initiation of md-ARV regimens increased from 35.5% to 97% during this period. In 2008, women attending ANC at <it>full package </it>sites were 30% more likely to undergo CD4 cell count assessment during pregnancy than women attending <it>stand-alone </it>sites (relative risk (RR) = 1.3; 95% confidence interval (CI): 1.1-1.4). Enrolment of HIV+ pregnant women in ART services was almost twice as likely at <it>full package </it>sites than at <it>stand-alone </it>sites (RR = 1.9; 95% CI: 1.5-2.3). However, no significant differences were detected between the two models of care in providing md-ARV (RR = 0.9; 95% CI: 0.9-1.0).</p> <p>Conclusions</p> <p>All sites successfully transitioned from sd-nvp to md-ARV regimens for PMTCT. <it>Full package </it>sites offer the most efficient model for providing immunological assessment and enrolment into care and treatment of HIV+ pregnant women. Strengthening the capacity of <it>stand-alone </it>PMTCT sites to achieve the same objectives is paramount.</p

Evidence for an enhanced HIV/AIDS policy and care in Cameroon: proceedings of the second Cameroon HIV Research Forum (CAM-HERO) 2021

To achieve the Sustainable Development Goal of zero hunger, multi-sectoral strategies to improve nutrition are necessary. Building towards this goal, the food and agriculture sector must be considered when designing nutritional interventions. Nevertheless, most frameworks designed to guide nutritional interventions do not adequately capture opportunities for integrating nutrition interventions within the food and agriculture sector. This paper aims to highlight how deeply connected the food and agriculture sector is to underlying causes of malnutrition and identify opportunities to better integrate the food and agriculture sector and nutrition in low and middle income countries. In particular, this paper: (1) expands on the UNICEF conceptual framework for undernutrition to integrate the food and agriculture sector and nutrition outcomes, (2) identifies how nutritional outcomes and agriculture are linked in six important ways by defining evidence-based food and agriculture system components within these pathways: as a source of food, as a source of income, through food prices, women’s empowerment, women’s utilization of time, and women’s health and nutritional status, and (3) shows that the food and agriculture sector facilitates interventions through production, processing and consumption, as well as through farmer practices and behavior. Current frameworks used to guide nutrition interventions are designed from a health sector paradigm, leaving agricultural aspects not sufficiently leveraged. This paper concludes by proposing intervention opportunities to rectify the missed opportunities generated by this approach. Program design should consider the ways that the food and agriculture sector is linked to other critical sectors to comprehensively address malnutrition. This framework is designed to help the user to begin to identify intervention sites that may be considered when planning and implementing multi-sectoral nutrition program

Whole-cell pertussis vaccine induces low antibody levels in human immunodeficiency virus-infected children living in sub-Saharan Africa.

International audienceThe WHO recommendations for the immunization of children infected with human immunodeficiency virus (HIV) differ slightly from the guidelines for uninfected children. The introduction of antiretroviral therapy for HIV-infected infants should considerably prolong their life expectancy. The question of the response to the whole-cell pertussis (wP) vaccine should now be addressed, particularly in countries in which pertussis remains endemic. To evaluate the persistence of antibodies to the wP vaccine in HIV-infected and uninfected children who had previously received this vaccine in routine clinical practice, we conducted a cross-sectional study of children aged 18 to 36 months, born to HIV-infected mothers and living in Cameroon or the Central African Republic. We tested blood samples for antibodies to the wP vaccine and for antibodies to diphtheria and tetanus toxoids (D and T, respectively) in the context of the use of a combined DTwP vaccine. We enrolled 50 HIV-infected children and 78 uninfected, HIV-exposed children in the study. A lower proportion of HIV-infected children than uninfected children had antibodies against the antigens tested for all valences of the DTwP vaccine. Agglutinin levels were substantially lower in HIV-infected than in HIV-exposed but uninfected children (30.0% versus 55.1%, respectively; P = 0.005). We also observed a high risk of low antibody levels in response to the DTwP vaccine in HIV-infected children with severe immunodeficiency (CD4 T-cell level, <25%). The concentrations of antibodies induced by the DTwP vaccine were lower in HIV-infected children than in uninfected children. This study supports the need for a booster dose of the DTwP vaccine in order to maintain high antibody levels in HIV-infected children

HIV-infected children living in Central Africa have low persistence of antibodies to vaccines used in the Expanded Program on Immunization.

International audienceBACKGROUND: The Expanded Program on Immunization (EPI) is the most cost-effective measures to control vaccine-preventable diseases. Currently, the EPI schedule is similar for HIV-infected children; the introduction of antiretroviral therapy (ART) should considerably prolong their life expectancy. METHODS AND PRINCIPAL FINDINGS: To evaluate the persistence of antibodies to the EPI vaccines in HIV-infected and HIV-exposed uninfected children who previously received these vaccines in routine clinical practice, we conducted a cross-sectional study of children, aged 18 to 36 months, born to HIV-infected mothers and living in Central Africa. We tested blood samples for antibodies to the combined diphtheria, tetanus, and whole-cell pertussis (DTwP), the measles and the oral polio (OPV) vaccines. We enrolled 51 HIV-infected children of whom 33 were receiving ART, and 78 HIV-uninfected children born to HIV-infected women. A lower proportion of HIV-infected children than uninfected children had antibodies to the tested antigens with the exception of the OPV types 1 and 2. This difference was substantial for the measles vaccine (20% of the HIV-infected children and 56% of the HIV-exposed uninfected children, p<0.0001). We observed a high risk of low antibody levels for all EPI vaccines, except OPV types 1 and 2, in HIV-infected children with severe immunodeficiency (CD4(+) T cells <25%). CONCLUSIONS AND SIGNIFICANCE: Children were examined at a time when their antibody concentrations to EPI vaccines would have still not undergone significant decay. However, we showed that the antibody concentrations were lowered in HIV-infected children. Moreover, antibody concentration after a single dose of the measles vaccine was substantially lower than expected, particularly low in HIV-infected children with low CD4(+) T cell counts. This study supports the need for a second dose of the measles vaccine and for a booster dose of the DTwP and OPV vaccines to maintain the antibody concentrations in HIV-infected and HIV-exposed uninfected children

Low antibody concentrations or titers to the EPI antigens in relation to the percentage of CD4⁺ T cells.

*<p>Odds ratio</p>†<p>Confidence interval</p

Association between measles antibody concentration and children's characteristics.

*<p>Odds ratio of low measles antibody response</p>†<p>Confidence interval</p>‡<p>Chi square test, Fisher exact test or chi square test for trend as appropriate</p>§<p>Median age</p>||<p>Mid upper arm circumference</p>¶<p>Mean globular volume</p

Logistic regression analysis of various characteristics of children in relation to low antibody concentrations or titers to EPI vaccines (HIV-exposed uninfected children as reference group).

†<p>Adjusted Odds Ratio</p>‡<p>Confidence interval</p