<i>Trichoderma</i> volatiles effecting <i>Arabidopsis</i>:from inhibition to protection against phytopathogenic fungi

Trichoderma species are present in many ecosystems and some strains have the ability to reduce the severity of plant diseases by activating various defence pathways via specific biologically active signaling molecules. Hence we investigated the effects of low molecular weight volatile compounds of Trichoderma asperellum IsmT5 on Arabidopsis thaliana. During co-cultivation of T. asperellum IsmT5 without physical contact to A. thaliana we observed smaller but vital and robust plants. The exposed plants exhibit increased trichome numbers, accumulation of defence-related compounds such as H2O2, anthocyanin, camalexin, and increased expression of defence related genes. We conclude that A. thaliana perceives the Trichoderma volatiles as stress compounds and subsequently initiates multilayered adaptations including activation of signaling cascades to withstand this environmental influence.The prominent headspace volatile of T. asperellum IsmT5 was identified to be 6-pentyl-α-pyrone, which was solely applied to A. thaliana to verify the growth and defence reactions. Most noticeable is that A. thaliana preexposed to 6PP showed significantly reduced symptoms when challenged with Botrytis cinerea and Alternaria brassicicola, indicating that defence-activated plants subsequently became more resistant to pathogen attack. Together, these results support that products that are based on Trichoderma volatiles have the potential being a useful biocontrol agent in agriculture

Bimodal Distribution of Area-Weighted Latitude of Sunspots And Solar North-South Asymmetry

We study the latitudinal distribution of sunspots observed from 1874 to 2009 using the center-of-latitude (COL). We calculate COL by taking the area-weighted mean latitude of sunspots for each calendar month. We then form the latitudinal distribution of COL for the sunspots appearing in the northern and southern hemispheres separately, and in both hemispheres with unsigned and signed latitudes, respectively. We repeat the analysis with subsets which are divided based on the criterion of which hemisphere is dominant for a given solar cycle. Our primary findings are as follows: (1) COL is not monotonically decreasing with time in each cycle. Small humps can be seen (or short plateaus) around every solar maxima. (2) The distribution of COL resulting from each hemisphere is bimodal, which can well be represented by the double Gaussian function. (3) As far as the primary component of the double Gaussian function is concerned, for a given data subset, the distributions due to the sunspots appearing in two different hemispheres are alike. Regardless of which hemisphere is magnetically dominant, the primary component of the double Gaussian function seems relatively unchanged. (4) When the northern (southern) hemisphere is dominant the width of the secondary component of the double Gaussian function in the northern (southern) hemisphere case is about twice as wide as that in the southern (northern) hemisphere. (5) For the distribution of the COL averaged with signed latitude, whose distribution is basically described by a single Gaussian function, it is shifted to the positive (negative) side when the northern (southern) hemisphere is dominant. Finally, we conclude by briefly discussing the implications of these findings on the variations in the solar activity.Comment: Accepted for publication in New Astronom

Genetic architecture of glucosinolate variation in Brassica napus

The diverse biological activities of glucosinolate (GSL) hydrolysis products play significant biological and economical roles in the defense system and nutritional qualities of Brassica napus (oilseed rape). Yet, genomic-based study of the B. napus GSL regulatory mechanisms are scarce due to the complexity of working with polyploid species. To address these challenges, we used transcriptome-based GWAS approach, Associative Transcriptomics (AT), across a diversity panel of 288 B. napus genotypes to uncover the underlying genetic basis controlling quantitative variation of GSLs in B. napus vegetative tissues. Single nucleotide polymorphism (SNP) markers and gene expression markers (GEMs) associations identify orthologues of MYB28/HAG1 (AT5G61420), specifically the copies on chromosome A9 and C2, to be the key regulators of aliphatic GSL variation in leaves. We show that the positive correlation observed between aliphatic GSLs in seed and leaf is due to the amount synthesized, as controlled by Bna.HAG1.A9 and Bna.HAG1.C2, rather than by variation in the transport processes. In addition, AT and differential expression analysis in root tissues implicate an orthologue of MYB29/HAG3 (AT5G07690), Bna.HAG3.A3, as controlling root aromatic GSL variation. Based on the root expression data we also propose Bna.MAM3.A3 to have a role in controlling phenylalanine chain elongation for aromatic GSL biosynthesis. This work uncovers a regulator of homophenylalanine-derived aromatic GSLs and implicates the shared biosynthetic pathways between aliphatic and aromatic GSLs

A transposon-based activation-tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations

AbstractA population of 9471 stable activation-tagged lines was generated by transposable element mediated activation tagging mutagenesis in Arabidopsis (TAMARA) using the maize En/Spm transposon system. Based on DNA gel blot and flanking sequence analysis, this population contains approximately 6000 independent transposon insertions. A greenhouse-based screen identified six dominant or semi-dominant activation tagged mutants with obvious developmental alterations, among these a new pistillata mutant allele. In addition, a subset of 1500 lines was screened by a HPLC based high-throughput method for dominant activation tagged mutants with enhanced contents of phenolic compounds. One dominant activation tagged mutant (hpc1-1D) was isolated showing accumulation of a particular compound due to the upregulation of an R2R3-MYB transcription factor

Bioavailability of Glucoraphanin and Sulforaphane From High-Glucoraphanin Broccoli

Scope: Broccoli accumulates 4-methylsulphinylbutyl glucosinolate (glucoraphanin) which is hydrolyzed to the isothiocyanate sulforaphane. Through the introgression of novel alleles of the Myb28 transcription factor from Brassica villosa, broccoli genotypes have been developed that have enhanced levels of glucoraphanin. This study sought to quantify the exposure of human tissues to glucoraphanin and sulforaphane following consumption of broccoli with contrasting Myb28 genotypes. Methods and results: Ten participants were recruited into a three-phase, double-blinded, randomized crossover trial (NCT02300324), with each phase comprising consumption of 300 ml of a soup made from broccoli of one of three Myb28 genotypes (Myb28B/B, Myb28B/V, Myb28V/V). Plant myrosinases were intentionally denatured during soup manufacture. Three-fold and five-fold higher levels of sulforaphane occurred in the circulation following consumption of Myb28V/B and Myb28V/V broccoli soups, respectively. The percentage of sulforaphane excreted in 24 h relative to the amount of glucoraphanin consumed varied amongst volunteers from 2% to 15%, but did not depend on the broccoli genotype. Conclusion: This is the first study to report the bioavailability of glucoraphanin and sulforaphane from soups made with novel broccoli varieties. The presence of one or two Myb28V alleles results in enhanced delivery of sulforaphane to the systemic circulation

Independent responses to ultraviolet radiation and herbivore attack in broccoli

The plant responses to ultraviolet-B radiation (UV-B) and to insect herbivory are believed to be partially similar. In this study, responses to these factors were investigated in the crop species broccoli (Brassica oleracea L. convar. botrytis, Brassicaceae). Plants were first grown under three UV-B regimes (80%, 23%, and 4% transmittance of ambient UV-B) in greenhouses covered with either innovative materials (high and medium transmittance) or conventional glass (low transmittance). Half of the plants then remained under these conditions, but the other half were transferred to the field with ambient light and herbivore access for up to 3 d. The plant responses to distinct environmental conditions were examined by analysing the morphological and chemical parameters of plants kept inside and plants exposed in the field. Furthermore, suitability of field-exposed plants to naturally occurring insects was investigated in relation to UV-B pretreatment. High levels of UV-B radiation led to increased flavonoid concentrations, but to a lower biomass accumulation in broccoli. These patterns remained after outdoor exposure. However, UV-induced changes of plant traits did not alter attractiveness to herbivorous insects: thrips, whiteflies, and aphids attacked plants independently of UV-B pretreatment. A 3-fold increase of indolyl glucosinolate concentrations occurred in above-ground tissue of all the plants, most likely due to massive herbivore attack after 3 d of field exposure. The results show that plants respond with high specificity to different abiotic and biotic impacts, demonstrating the separate perception and processing of stress factors