Increased numbers of oligodendrocyte lineage cells in the optic nerves of cerebroside sulfotransferase knockout mice

Sulfatide is a myelin glycolipid that functions in the formation of paranodal axo-glial junctions in vivo and in the regulation of oligodendrocyte differentiation in vitro. Cerebroside sulfotransferase (CST) catalyzes the production of two sulfated glycolipids, sulfatide and proligodendroblast antigen, in oligodendrocyte lineage cells. Recent studies have demonstrated significant increases in oligodendrocytes from the myelination stage through adulthood in brain and spinal cord under CST-deficient conditions. However, whether these result from excess migration or in situ proliferation during development is undetermined. In the present study, CST-deficient optic nerves were used to examine migration and proliferation of oligodendrocyte precursor cells (OPCs) under sulfated glycolipid-deficient conditions. In adults, more NG2-positive OPCs and fully differentiated cells were observed. In developing optic nerves, the number of cells at the leading edge of migration was similar in CST-deficient and wild-type mice. However, BrdU+ proliferating OPCs were more abundant in CST-deficient mice. These results suggest that sulfated glycolipids may be involved in proliferation of OPCs in vivo

Distribution patterns of tau pathology in progressive supranuclear palsy

Progressive supranuclear palsy (PSP) is a 4R-tauopathy predominated by subcortical pathology in neurons, astrocytes, and oligodendroglia associated with various clinical phenotypes. In the present international study, we addressed the question of whether or not sequential distribution patterns can be recognized for PSP pathology. We evaluated heat maps and distribution patterns of neuronal, astroglial, and oligodendroglial tau pathologies and their combinations in different clinical subtypes of PSP in postmortem brains. W

Alberta CancerBridges Development of a Care Plan Evaluation Measure

Background: No standardized measures specifically assess cancer survivors’ and healthcare providers’ experience of Survivor Care Plans (SCPS). We sought to develop two care plan evaluation (CPE) measures, one for survivors (CPE-S) and one for healthcare providers (CPE-P), examine initial psychometric qualities in Alberta, and assess generalizability in Manitoba, Canada. Methods: We developed the initial measures using convenience samples of breast (n = 35) and head and neck (n = 18) survivors who received scps at the end of active cancer-centre treatment. After assessing Alberta’s scp concordance with Institute of Medicine (IOM) recommendations using a published coding scheme, we examined psychometric qualities for the CPE-S and CPE-P. We examined generalizability in Manitoba, Canada, with colorectal survivors discharged to primary care providers for follow-up (n = 75). Results: We demonstrated acceptable internal consistency for the cpe-s and cpe-p subscales and total score after eliminating one item per subscale for cpe-s, two for cpe-p, resulting in revised scales with four 7-item and 6-item subscales, respectively. Subscale scores correlated highly indicating that for each measure the total score may be the most reliable and valid. We provide initial cpe-s discriminant, convergent, and predictive validity using the total score. Using the Manitoba sample, initial psychometrics similarly indicated good generalizability across differences in tumour groups, scp, and location. Conclusions: We recommend the revised cpe-s and cpe-p for further use and development. Studies documenting the creation and standardization of scp evaluations are few, and we recommend further development of patient experience measures to improve both clinical practice and the specificity of research questions

Automated Co-evolution of GMF Editor Models

Abstract. The Eclipse Graphical Modeling (GMF) Framework provides the ma-jor approach for implementing visual languages on top of the Eclipse platform. GMF relies on a family of modeling languages to describe different aspects of the visual language and its implementation in an editor. GMF uses a model-driven approach to map the different GMF models to Java code. The framework, as it stands, provides very little support for evolution. In particular, there is no support for propagating changes from say the domain model (i.e., the abstract syntax of the visual language) to other models. We analyze the resulting co-evolution chal-lenge, and we provide a transformation-based solution, say GMF model adapters, that serve the propagation of abstract-syntax changes based on the interpretation of difference models.