Bacteria Use Type IV Pili to Walk Upright and Detach from Surfaces

1. Department of Bioengineering, California Nano Systems Institute,University of California, Los Angeles, CA 90024, USA. 2. Department of Chemical and Biomolecular Engineering, University of Houston, Houston, TX 77204, USA. 3. Department of Materials Science and Engineering, University of Illinois, Urbana-Champaign, IL 61801, USA. 4. Department of Civil Engineering and Geological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.Bacterial biofilms are structured multicellular communities involved in a broad range of infections. Knowing how free-swimming bacteria adapt their motility mechanisms near surfaces is crucial for understanding the transition between planktonic and biofilm phenotypes. By translating microscopy movies into searchable databases of bacterial behavior, we identified fundamental type IV pili–driven mechanisms for Pseudomonas aeruginosa surface motility involved in distinct foraging strategies. Bacteria stood upright and “walked” with trajectories optimized for two-dimensional surface exploration. Vertical orientation facilitated surface detachment and could influence biofilm morphology.Center for Nonlinear Dynamic

Cool Companions to White Dwarf Stars from the Two Micron All Sky Survey All Sky Data Release

We present the culmination of our near-infrared survey of the optically spectroscopically identified white dwarf stars from the McCook and Sion catalog, conducted using photometric data from the Two Micron All Sky Survey final All Sky Data Release. The color selection technique, which identifies candidate binaries containing a white dwarf and a low-mass stellar (or substellar) companion via their distinctive locus in the near-infrared color-color diagram, is demonstrated to be simple to apply and to yield candidates with a high rate of subsequent confirmation. We recover 105 confirmed binaries, and identify 27 firm candidates (19 of which are new to this work) and 21 tentative candidates (17 of which are new to this work) from the 2MASS data. Only a small number of candidates from our survey have likely companion spectral types later than M5, none of which is an obvious L-type (i.e., potential brown dwarf) companion. Only one previously known white dwarf + brown dwarf binary is detected. This result is discussed in the context of the 2MASS detection limits, as well as other recent observational surveys that suggest a very low rate of formation (or survival) for binary stars with extreme mass ratios

Flagella and Pili-Mediated Near-Surface Single-Cell Motility Mechanisms in P. aeruginosa

Bacterial biofilms are structured multicellular communities that are responsible for a broad range of infections. Knowing how free-swimming bacteria adapt their motility mechanisms near a surface is crucial for understanding the transition from the planktonic to the biofilm phenotype. By translating microscopy movies into searchable databases of bacterial behavior and developing image-based search engines, we were able to identify fundamental appendage-specific mechanisms for the surface motility of Pseudomonas aeruginosa. Type IV pili mediate two surface motility mechanisms: horizontally oriented crawling, by which the bacterium moves lengthwise with high directional persistence, and vertically oriented walking, by which the bacterium moves with low directional persistence and high instantaneous velocity, allowing it to rapidly explore microenvironments. The flagellum mediates two additional motility mechanisms: near-surface swimming and surface-anchored spinning, which often precedes detachment from a surface. Flagella and pili interact cooperatively in a launch sequence whereby bacteria change orientation from horizontal to vertical and then detach. Vertical orientation facilitates detachment from surfaces and thereby influences biofilm morphology.Physic

Flagella and Pili-Mediated Near-Surface Single-Cell Motility Mechanisms in P. aeruginosa

Bacterial biofilms are structured multicellular communities that are responsible for a broad range of infections. Knowing how free-swimming bacteria adapt their motility mechanisms near a surface is crucial for understanding the transition from the planktonic to the biofilm phenotype. By translating microscopy movies into searchable databases of bacterial behavior and developing image-based search engines, we were able to identify fundamental appendage-specific mechanisms for the surface motility of Pseudomonas aeruginosa. Type IV pili mediate two surface motility mechanisms: horizontally oriented crawling, by which the bacterium moves lengthwise with high directional persistence, and vertically oriented walking, by which the bacterium moves with low directional persistence and high instantaneous velocity, allowing it to rapidly explore microenvironments. The flagellum mediates two additional motility mechanisms: near-surface swimming and surface-anchored spinning, which often precedes detachment from a surface. Flagella and pili interact cooperatively in a launch sequence whereby bacteria change orientation from horizontal to vertical and then detach. Vertical orientation facilitates detachment from surfaces and thereby influences biofilm morphology