On-farm experimentation in agronomic research: an Italian perspective

On-farm experimentation (OFE) renovates agronomic research since it brings researchers out of their experimental field stations to the farms, shaping the direction of research in collaboration with farmers. In the context of increasing interest in OFE, this paper aims to map the current picture of agronomic research articles published on OFE in Italy. We observe that few articles are published on OFE in Italy. Moreover, among these articles, only a few explicitly mentioned farmers’ opinions or involvement, while none of them mentioned digital technologies as enablers of OFE. Therefore, we started a SWOT (Strengths, Weaknesses, Opportunities, and Threats) analysis to identify the main weaknesses and threats limiting OFE developing in Italy, along with the opportunities and strengths enabling OFE development. Despite the time consuming research underpinning OFE, the lack of recognition by the academic community, the risk of not robust statistic, and difficulties in publishing, there are a series of opportunities emerging at the national level for which the implementation of OFE could be crucial to properly target the scopes set by the EU concerning agricultural research and innovation

A multi-adaptive framework for the crop choice in paludicultural cropping systems

The conventional cultivation of drained peatland causes peat oxidation, soil subsidence, nutrient loss, increasing greenhouse gas emissions and biodiversity reduction. Paludiculture has been identified as an alternative management strategy consisting in the cultivation of biomass on wet and rewetted peatlands. This strategy can save these habitats and restore the ecosystem services provided by the peatlands both on the local and global scale. This paper illustrates the most important features to optimise the crop choice phase, which is the crucial point for the success of paludiculture systems. A multi-adaptive framework was proposed. It was based on four points that should be checked to identify suitable crops for paludicultural cropping system: biological traits, biomass production, attitude to cultivation and biomass quality. The main agronomic implications were explored with the help of some results from a plurennial open-field experimentation carried out in a paludicultural system set up in the Massaciuccoli Lake Basin (Tuscany, Italy) and a complete example of the method application was provided. The tested crops were Arundo donax L., Miscanthus × giganteus Greef et Deuter, Phragmites australis L., Populus × canadensis Moench. and Salix alba L. The results showed a different level of suitability ascribable to the different plant species proving that the proposed framework can discriminate the behaviour of tested crops. Phragmites australis L.was the most suitable crop whereas Populus × canadensis Moench and Miscanthus × giganteus Greef et Deuter (in the case of biogas conversion) occupied the last positions in the ranking

Rewetting in Mediterranean reclaimed peaty soils and its potential for phyto-treatment use

A pilot experimental field combining rewetting of reclaimed peaty soils and water phyto-treatment was set up in the Massaciuccoli Lake basin (Tuscany, Italy) to reduce the water eutrophication and peat degradation caused by almost a century of drainage-based agricultural use. In this paper, we investigated the restoration process occurring consequently to the conversion of a drained area in a natural wetland system (NWS) (the partial top soil removal, the realization of a perimeter levee to contain the waters, the rewetting with the drainage waters coming from the of surrounding cultivated areas) and the capability of the spontaneous vegetation to catch nutrients acting as a vegetation filter. To follow the restoration process over time (2012e2016), we used a mixed approach merging phytosociological surveys with ortophotos taken by an Unmanned Aerial Vehicle (UAV). During the last year of observation (2016), we performed destructive sampling on the most widespread plant communities in the area (Phragmites australis and Myriophyllum aquaticum community) to quantify the biomass production and the uptake of nitrogen and phosphorus. Stands of Phragmites australis (Cav.) Trin. ex Steud. yielded more than Myriophyllum aquaticum (Vell.) Verdc. (4.94 kg m-2 vs 1.08 kg m-2). M. aquaticum showed higher nutrient contents (2.04% of N and 0.35% of P), however P. australis was able to take up more nutrients within the NWS because of its larger cover and productivity. In the perspective of maximizing the plant development and consequently the amount of nutrients extracted from treated waters, the authors suggest 4-5 year-long-harvesting turns, better occurring in spring-summer

“A case of ecological renaturation in a drained Mediterranean peatland: the case study of the Massaciuccoli Lake basin (Tuscany, It)”

The Massaciuccoli Lake floodplain is located in the Natural Park of San Rossore, Migliarino and Massaciuccoli in NW of Tuscany (Italy). Since the 1930s, a large part of the Massaciuccoli floodplain has been drained for agricultural purposes. To ensure a water table depth suitable for cultivation, a complex network of artificial drains and pumping stations has been used to drain the superficial aquifer and rainwater. Land use is characterised by conventional agriculture (covers 80% of the area) and periurban infrastructures, such as a wastewater treatment plant. In the peatland area, cropping systems are based on continuous production of maize (Zea mays L.), sunflower (Heliantus annuus L.), wheat (Triticum spp. L.). As a consequence of land use, several environmental concerns arose in the last 50 years. The most important concerns are those related to: I. eutrophication of the lake due to nutrient enrichment (N, P) in the surface- and groundwater. II. the subsidence rate (2-3 m in 70 years) due to compaction and increased mineralization of peat. The project RestoMedPeatland (https://sites.google.com/site/restomedpeatland/) started in 2011, identified rewetting and setting-up a phyto-treatment system as the solution for improving water quality,slowing down soil organic matter (SOM) mineralization, and, therefore, a method to restore the ecological functions of this site. A 15 ha experimental area was used to compare the efficiency of three different systems (A: constructed wetland, B: paludiculture system and C: natural wetland in treating the eutrophic drainage water coming from a sub-watershed in the reclamation district.In the natural wetland (C), after top soil removal, excavation and rewetting with drainage water, the vegetation has evolved naturally. The surveys carried out during the four years after pilot experimental field, highlighted the development of a spontaneous hydro-hygrophilous vegetation. This was mainly composed of helophytic communities dominated by Phragmites australis (Cav.) Trin. and Typha latifolia L., in addition to monophytic populations of Myriophillum spp., occupying different areas of the system according to the depth of excavation, for about 50% of the whole surface. The flora, currently, consists more than 30 species, as hydro-hygrophilous Alisma plantago-aquatica L., Juncus effusus L., Apium nodiflorum (L.) Lag, Lemna minor L., characteristic of the nearby Massaciuccoli Lake. The data collected so far showed a high and progressive growth of the biomass of the natural area, a dynamic upward trend of floristic-vegetation biodiversity, and, from a technical standpoint, a large phyto-treatment capability

Renaturation dynamics after the rewetting of a Mediterranean agricultural peatland

A new research project combining peatland rewetting and water phyto-treatment was set up in the Massaciuccoli Lake basin (Tuscany, Italy) to restore the peat degradation status and the water eutrophication conditions caused by almost a century of drained-based agricultural use. In a large scale phyto-treatment system, three different strategies (constructed wetland system, paludicultural system, natural wetland system) were studied to evaluate their efficiency in abating the nutrients loads from the drainage waters and to stop the peatland degradation. In this research, we investigated the renaturation process occurring within the natural wetland system as a result of the different disturbances carried out: the top soil removal in the perimeter area, the realization of a perimeter levee to contain the waters, the rewetting with the waters coming from the drainage of surrounding areas

Carbon Budget of an Agroforestry System after Being Converted from a Poplar Short Rotation Coppice

Poplar (Populus L. spp.) Short Rotation Coppice systems (SRCs) for bioenergy production are being converted back to arable land. Transitioning to Alley Cropping Systems (ACSs) could be a suitable strategy for integrating former tree rows and arable crops. A field trial (Pisa, Central Italy) was set up with the aim of assessing the C storage of an ACS system based on hybrid poplar and sorghum (Sorghum bicolor L. Moench) and comparing it with that of an SRC cultivation system. The carbon budget at the agroecosystem scale was assessed in the first year of the transition using the net biome production (NBP) approach with a simplified method. The overall NBP for the SRC was positive (96 40 g C m2 year1), highlighting that the system was a net carbon sink (i.e., NBP > 0). However, the ACS registered a net C loss (i.e., NBP < 0), since the NBP was 93 56 g C m2 year1. In the first year of the transition, converting the SRC into an ACS counteracted the potential beneficial eect of C storage in tree belowground biomass due to the high heterotrophic respiration rate recorded in the ACS, which was fostered by the incorporation of residues and tillage disturbance in the alley. Additional years of heterotrophic respiration measurements could allow for an estimate of the speed and extent of C losses

Carbon Budget of an Agroforestry System after Being Converted from a Poplar Short Rotation Coppice

Poplar (Populus L. spp.) Short Rotation Coppice systems (SRCs) for bioenergy production are being converted back to arable land. Transitioning to Alley Cropping Systems (ACSs) could be a suitable strategy for integrating former tree rows and arable crops. A field trial (Pisa, Central Italy) was set up with the aim of assessing the C storage of an ACS system based on hybrid poplar and sorghum (Sorghum bicolor L. Moench) and comparing it with that of an SRC cultivation system. The carbon budget at the agroecosystem scale was assessed in the first year of the transition using the net biome production (NBP) approach with a simplified method. The overall NBP for the SRC was positive (96 ± 40 g C m−2 year−1), highlighting that the system was a net carbon sink (i.e., NBP > 0). However, the ACS registered a net C loss (i.e., NBP < 0), since the NBP was −93 ± 56 g C m−2 year−1. In the first year of the transition, converting the SRC into an ACS counteracted the potential beneficial effect of C storage in tree belowground biomass due to the high heterotrophic respiration rate recorded in the ACS, which was fostered by the incorporation of residues and tillage disturbance in the alley. Additional years of heterotrophic respiration measurements could allow for an estimate of the speed and extent of C losses

Endogenous calcium mediates seedling growth and fluoride stress tolerance in four bean genotypes

Fluoride (F) pollution is a global environmental problem representing a severe risk for food and vegetables grown in contaminated soils. Phaseolus vulgaris L. is widely cultivated in arid and semi-arid regions and in F contaminated areas of the world. For that reason, F tolerance during germination and seedling growth was evaluated for four bean genotypes: Borlotto nano (commercial variety) and three African genotypes (Lyamungu 85, Lyamungu 90 and Jesca). Seeds were grown in sand enriched with NaF or KF at three different levels (0, 80 and 200 mg kg-1). NaCl was used as a benchmark to determine the potential effect of different Na levels in plant. Total F content and mineral accumulation (Na, K and Ca) in roots and shoots were measured. The translocation factor, growth ratio, F tolerance index were evaluated to estimate plant-salt response. Germination rate decreased with increased F level. Borlotto was more F sensitive (0% germination with 200 mg kg-1of KF and NaF) than the African genotypes. Under the highest F concentration (200 mg kg-1), F preferentially accumulated in shoots (Jesca 75.7 mg kg-1, Lyamungu 85 100.1 mg kg-1 and Lyamungu 90 115.4 mg kg-1). Ca content in roots was negatively correlated to F absorption, suggesting its antagonistic role to F mobility. Based on these parameters, Jesca and Lyamungu 85 were the most tolerant species, recording a low F uptake and a high Ca content in the root. This study highlighted the central role of Ca, as a key secondary messenger in regulating the plant growth and development under F stress. Highlights - F stress negatively affected bean germination and seedling growth - F mainly accumulated in the shoots of bean varieties - Ca concentration in the roots played a crucial role in mitigating F accumulatio

Nano-topography:Quicksand for cell cycle progression?

The 3-D spatial and mechanical features of nano-topography can create alternative environments, which influence cellular response. In this paper, murine fibroblast cells were grown on surfaces characterized by protruding nanotubes. Cells cultured on such nano-structured surface exhibit stronger cellular adhesion compared to control groups, but despite the fact that stronger adhesion is generally believed to promote cell cycle progression, the time cells spend in G1 phase is doubled. This apparent contradiction is solved by confocal microscopy analysis, which shows that the nano-topography inhibits actin stress fiber formation. In turn, this impairs RhoA activation, which is required to suppress the inhibition of cell cycle progression imposed by p21/p27. This finding suggests that the generation of stress fibers, required to impose the homeostatic intracellular tension, rather than cell adhesion/spreading is the limiting factor for cell cycle progression. Indeed, nano-topography could represent a unique tool to inhibit proliferation in adherent well-spread cells

A gene dosage‐dependent effect unveils NBS1 as both a haploinsufficient tumour suppressor and an essential gene for SHH‐medulloblastoma

Aims: Inherited or somatic mutations in the MRE11, RAD50 and NBN genes increase the incidence of tumours, including medulloblastoma (MB). On the other hand, MRE11, RAD50 and NBS1 protein components of the MRN complex are often overexpressed and sometimes essential in cancer. In order to solve the apparent conundrum about the oncosuppressive or oncopromoting role of the MRN complex, we explored the functions of NBS1 in an MB-prone animal model. Materials and methods: We generated and analysed the monoallelic or biallelic deletion of the Nbn gene in the context of the SmoA1 transgenic mouse, a Sonic Hedgehog (SHH)-dependent MB-prone animal model. We used normal and tumour tissues from these animal models, primary granule cell progenitors (GCPs) from genetically modified animals and NBS1-depleted primary MB cells, to uncover the effects of NBS1 depletion by RNA-Seq, by biochemical characterisation of the SHH pathway and the DNA damage response (DDR) as well as on the growth and clonogenic properties of GCPs. Results: We found that monoallelic Nbn deletion increases SmoA1-dependent MB incidence. In addition to a defective DDR, Nbn+/- GCPs show increased clonogenicity compared to Nbn+/+ GCPs, dependent on an enhanced Notch signalling. In contrast, full NbnKO impairs MB development both in SmoA1 mice and in an SHH-driven tumour allograft. Conclusions: Our study indicates that Nbn is haploinsufficient for SHH-MB development whereas full NbnKO is epistatic on SHH-driven MB development, thus revealing a gene dosage-dependent effect of Nbn inactivation on SHH-MB development