Calorie restriction alters mitochondrial protein acetylation

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72130/1/j.1474-9726.2009.00503.x.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/72130/2/ACEL_503_sm_FigS1.pd

Calorie Restriction Alters Mitochondrial Protein Acetylation

Calorie restriction (CR) increases lifespan in organisms ranging from budding yeast through mammals. Mitochondrial adaptation represents a key component of the response to CR. Molecular mechanisms underlying this adaptation are largely unknown. Here we show that lysine acetylation of mitochondrial proteins is altered during CR in a tissue-specific fashion. Via large-scale mass spectrometry screening, we identify 72 candidate proteins involved in a variety of metabolic pathways with altered acetylation during CR. Mitochondrial acetylation changes may play an important role in the pro-longevity CR response

Tight function zonula occludens-3 regulates cyclin D1–dependent cell proliferation

We show here that zonula occludens (ZO)-3 acts to sequester cyclin D1 at intercellular tight junctions. This study highlights an important new role for ZO family proteins in regulating epithelial cell proliferation through stabilization of cyclin D1 during mitosis