Divergent Innate and Epithelial Functions of the RNA-Binding Protein HuR in Intestinal Inflammation

HuR is an abundant RNA-binding protein acting as a post-transcriptional regulator of many RNAs including mRNAs encoding inflammatory mediators, cytokines, death signalers and cell cycle regulators. In the context of intestinal pathologies, elevated HuR is considered to enhance the stability and the translation of pro-tumorigenic mRNAs providing the rationale for its pharmacological targeting. However, HuR also possesses specific regulatory functions for innate immunity and cytokine mRNA control which can oppose intestinal inflammation and tumor promotion. Here, we aim to identify contexts of intestinal inflammation where the innate immune and the epithelial functions of HuR converge or diverge. To address this, we use a disease-oriented phenotypic approach using mice lacking HuR either in intestinal epithelia or myeloid-derived immune compartments. These mice were compared for their responses to (a) Chemically induced Colitis; (b) Colitis- associated Cancer (CAC); (c) T-cell mediated enterotoxicity; (d) Citrobacter rodentium-induced colitis; and (e) TNF-driven inflammatory bowel disease. Convergent functions of epithelial and myeloid HuR included their requirement for suppressing inflammation in chemically induced colitis and their redundancies in chronic TNF-driven IBD and microbiota control. In the other contexts however, their functions diversified. Epithelial HuR was required to protect the epithelial barrier from acute inflammatory or infectious degeneration but also to promote tumor growth. In contrast, myeloid HuR was required to suppress the beneficial inflammation for pathogen clearance and tumor suppression. This cellular dichotomy in HuR's functions was validated further in mice engineered to express ubiquitously higher levels of HuR which displayed diminished pathologic and beneficial inflammatory responses, resistance to epithelial damage yet a heightened susceptibility to CAC. Our study demonstrates that epithelial and myeloid HuR affect different cellular dynamics in the intestine that need to be carefully considered for its pharmacological exploitation and points toward potential windows for harnessing HuR functions in intestinal inflammation

Photogrammetric surveying forests and woodlands with UAVs: Techniques for automatic removal of vegetation and Digital Terrain Model production for hydrological applications

The purpose of this study is the photogrammetric survey of a forested area using Unmanned Aerial Vehicles (UAV), and the estimation of the Digital Terrain Model (DTM) of the area, based on the photogrammetrically produced Digital Surface Model (DSM). Furthermore, through the classification of the height difference between a DSM and a DTM, a vegetation height model is estimated, and a vegetation type map is produced. Finally, the generated DTM was used in a hydrological analysis study in order to determine its suitability compared to the usage of the DSM. The selected study area was the forest of Seih-Sou (Thessaloniki). The DTM extraction methodology applies classification and filtering of point clouds, and aims in the production of a surface model including only terrain points (DTM). The method yielded a DTM which functioned satisfactorily as a basis for the hydrological analysis. Also, by classifying the DSM DTM difference, a vegetation heights model was generated. For the photogrammetric survey, 495 aerial images were used, taken by a UAV from a height of ~ 200m. A total of 44 ground control points were measured with an accuracy of 5cm. The accuracy of the aerial triangulation was approximately 13cm. The produced dense point cloud, counted 146,593,725 points.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author