3,925 research outputs found
Recurrent high-biomass blooms of Alexandrium taylorii (Dinophyceae), a HAB species expanding in the Mediterranean.
none9openGIACOBBE M.G., PENNA A., GANGEMI E., MASO' M; GARCES E., FRAGA S., BRAVO I., AZZARO F., PENNA N.Giacobbe, M. G.; Penna, Antonella; Gangemi, E.; Maso', M; Garces, E.; Fraga, S.; Bravo, I.; Azzaro, F.; Penna, N
Development of a qualitative PCR method for the Alexandrium (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)
Paralytic shellfish poisoning (PSP) is a syndrome caused by the consumption of shellfish contaminated with neurotoxins
produced by organisms of themarine dinoflagellate genus Alexandrium. A. minutum is the mostwidespread species responsible for PSP in theWestern Mediterranean basin. The standard monitoring of shellfish farms for the presence of harmful algae and related toxins usually requires the microscopic examination of phytoplankton populations, bioassays and toxin determination by HPLC. These procedures are time-consuming and require remarkable experience, thus limiting the number of specimens that can be analyzed by a single laboratory unit.Molecular biology techniques may be helpful in the detection of target microorganisms in field samples. In this study, we developed a qualitative PCR assay for the rapid detection of all potentially toxic species belonging to the Alexandrium genus and specifically A. minutum, in contaminated mussels. Alexandrium genus-specific primers were designed to target the 5.8S rDNA region, while an A. minutum species-specific primer was designed to bind in the ITS1 region. The assay was validated using several fixed seawater samples fromthe Mediterranean basin, which were analyzed using PCR along with standard microscopy procedures. The assay provided a rapid method for monitoring the presence of Alexandrium spp. in mussel tissues, as well as in seawater samples. The results showed that PCR is a valid, rapid alternative procedure for the detection of target phytoplankton species either in seawater or directly in mussels, where microalgae can accumulat
The GAPS Programme with HARPS-N at TNG XV. A substellar companion around a K giant star identified with quasi-simultaneous HARPS-N and GIANO measurements
Context. Identification of planetary companions of giant stars is made
difficult because of the astrophysical noise, that may produce radial velocity
(RV) variations similar to those induced by a companion. On the other hand any
stellar signal is wavelength dependent, while signals due to a companion are
achromatic. Aims. Our goal is to determine the origin of the Doppler periodic
variations observed in the thick disk K giant star TYC 4282-605-1 by HARPS-N at
the Telescopio Nazionale Galileo (TNG) and verify if they can be due to the
presence of a substellar companion. Methods. Several methods have been used to
exclude the stellar origin of the observed signal including detailed analysis
of activity indicators and bisector and the analysis of the photometric light
curve. Finally we have conducted an observational campaign to monitor the near
infrared (NIR) RV with GIANO at the TNG in order to verify whether the NIR
amplitude variations are comparable with those observed in the visible.
Results. Both optical and NIR RVs show consistent variations with a period at
101 days and similar amplitude, pointing to the presence of a companion
orbiting the target. The main orbital properties obtained for our giant star
with a derived mass of M=0.97+-0.03M_sun are
M_Psini=10.78+-0.12MJ;P=101.54+-0.05days;e=0.28+-0.01 and a=0.422+-0.009AU. The
chemical analysis shows a significant enrichment in the abundance of Nai, Mgi,
Ali and S i while the rest of analyzed elements are consistent with the solar
value demonstrating that the chemical composition corresponds with an old K
giant (age = 10.1 Gyr) belonging to local thick disk. Conclusions. We conclude
that the substellar companion hypothesis for this K giant is the best
explanation for the observed periodic RV variation. This study also shows the
high potential of multi-wavelength RV observations for the validation of planet
candidates.Comment: Accepted in Journal reference A&A 14/06/201
Accuracy of elastic fusion biopsy in daily practice: results of a multicenter study of 2115 patients
OBJECTIVES:
To assess the accuracy of Koelis fusion biopsy for the detection of prostate cancer and clinically significant prostate cancer in the everyday practice.
METHODS:
We retrospectively enrolled 2115 patients from 15 institutions in four European countries undergoing transrectal Koelis fusion biopsy from 2010 to 2017. A variable number of target (usually 2-4) and random cores (usually 10-14) were carried out, depending on the clinical case and institution habits. The overall and clinically significant prostate cancer detection rates were assessed, evaluating the diagnostic role of additional random biopsies. The cancer detection rate was correlated to multiparametric magnetic resonance imaging features and clinical variables.
RESULTS:
The mean number of targeted and random cores taken were 3.9 (standard deviation 2.1) and 10.5 (standard deviation 5.0), respectively. The cancer detection rate of Koelis biopsies was 58% for all cancers and 43% for clinically significant prostate cancer. The performance of additional, random cores improved the cancer detection rate of 13% for all cancers (P < 0.001) and 9% for clinically significant prostate cancer (P < 0.001). Prostate cancer was detected in 31%, 66% and 89% of patients with lesions scored as Prostate Imaging Reporting and Data System 3, 4 and 5, respectively. Clinical stage and Prostate Imaging Reporting and Data System score were predictors of prostate cancer detection in multivariate analyses. Prostate-specific antigen was associated with prostate cancer detection only for clinically significant prostate cancer.
CONCLUSIONS:
Koelis fusion biopsy offers a good cancer detection rate, which is increased in patients with a high Prostate Imaging Reporting and Data System score and clinical stage. The performance of additional, random cores seems unavoidable for correct sampling. In our experience, the Prostate Imaging Reporting and Data System score and clinical stage are predictors of prostate cancer and clinically significant prostate cancer detection; prostate-specific antigen is associated only with clinically significant prostate cancer detection, and a higher number of biopsy cores are not associated with a higher cancer detection rate
HADES RV Programme with HARPS-N at TNG. VII. Rotation and activity of M-Dwarfs from time-series high-resolution spectroscopy of chromospheric indicators
We aim to investigate the presence of signatures of magnetic cycles and
rotation on a sample of 71 early M-dwarfs from the HADES RV programme using
high-resolution time-series spectroscopy of the Ca II H & K and Halpha
chromospheric activity indicators, the radial velocity series, the parameters
of the cross correlation function and the V-band photometry. We used mainly
HARPS-N spectra, acquired over four years, and add HARPS spectra from the
public ESO database and ASAS photometry light-curves as support data, extending
the baseline of the observations of some stars up to 12 years. We provide
log(R'hk) measurements for all the stars in the sample, cycle length
measurements for 13 stars, rotation periods for 33 stars and we are able to
measure the semi-amplitude of the radial velocity signal induced by rotation in
16 stars. We complement our work with previous results and confirm and refine
the previously reported relationships between the mean level of chromospheric
emission, measured by the log(R'hk), with the rotation period, and with the
measured semi-amplitude of the activity induced radial velocity signal for
early M-dwarfs. We searched for a possible relation between the measured
rotation periods and the lengths of the magnetic cycle, finding a weak
correlation between both quantities. Using previous v sin i measurements we
estimated the inclinations of the star's poles to the line of sight for all the
stars in the sample, and estimate the range of masses of the planets GJ 3998 b
and c (2.5 - 4.9 Mearth and 6.3 - 12.5 Mearth), GJ 625 b (2.82 Mearth), GJ 3942
b (7.1 - 10.0 Mearth) and GJ 15A b (3.1 - 3.3 Mearth), assuming their orbits
are coplanar with the stellar rotation.Comment: 19 pages, 16 figures, 10 table
The HADES RV Programme with HARPS-N@TNG II. Data treatment and simulations
The distribution of exoplanets around low-mass stars is still not well
understood. Such stars, however, present an excellent opportunity of reaching
down to the rocky and habitable planet domains. The number of current
detections used for statistical purposes is still quite modest and different
surveys, using both photometry and precise radial velocities, are searching for
planets around M dwarfs. Our HARPS-N red dwarf exoplanet survey is aimed at the
detection of new planets around a sample of 78 selected stars, together with
the subsequent characterization of their activity properties. Here we
investigate the survey performance and strategy. From 2700 observed spectra, we
compare the radial velocity determinations of the HARPS-N DRS pipeline and the
HARPS-TERRA code, we calculate the mean activity jitter level, we evaluate the
planet detection expectations, and we address the general question of how to
define the strategy of spectroscopic surveys in order to be most efficient in
the detection of planets. We find that the HARPS-TERRA radial velocities show
less scatter and we calculate a mean activity jitter of 2.3 m/s for our sample.
For a general radial velocity survey with limited observing time, the number of
observations per star is key for the detection efficiency. In the case of an
early M-type target sample, we conclude that approximately 50 observations per
star with exposure times of 900 s and precisions of about 1 m/s maximizes the
number of planet detections
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