Tri nove vrste i jedan novi rod ultraspecijaliziranih špiljskih leptodirina iz Hrvatske (Coleoptera, Cholevidae)

Croatodirus casalei Giachino & Jalžić, new species from N Velebit, Lubenovac, Slovačka jama pothole, and C. ozimeci Casale, Giachino & Jalžić new species, from Lokve, Lokvarka špilja cave, are described. The morphological features of the new taxa are compared with those of the type species of the genus (C. bozicevici Casale, Giachino & Jalžić, 2000). The genus is confirmed as a homogeneous, well characterized and monophyletic unit, and is attributed to the phyletic lineage of Anthroherpon. Velebitodromus, new genus, smidai new species, is described from N Velebit, Lubenovac, Mali kuk, Slovačka jama pothole. Owing to both external features and structures of male and female genitalia, the genus is attributed to the phyletic lineage of Anthroherpon, and is recognized as related to the genera Anthroherpon Reitter, 1889, and Paranthrophilon Reitter, 1889.U radu su opisane Croatodirus casalei Giachino & Jalžić, nova vrsta sa Sjevernog Velebita (Lubenovac, Mali kuk, Slovačka jama) i C. ozimeci Casale, Giachino & Jalžić, nova vrsta iz Lokava (špilja Lokvarka). Njihova morfološka svojstva uspoređuju se s onima tipske vrste ovog roda (C. bozicevici Casale, Giachino & Jalžić, 2000). Rod se potvrđuje kao homogen, jasno raspoznatljiv i monofiletički, te je pridodan filetičkoj liniji Anthroherpon. Novi rod Velebitodromus s novom vrstom smidai opisan je također sa Sjevernog Velebita (Lubenovac, Slovačka jama). Zbog vanjskih osobina i zbog građe muških i ženskih genitalija, rod je pridodan filetičkoj liniji Anthroherpon, i prepoznaje se kao srodan rodovima Anthroherpon Reitter, 1889 i Paranthrophilon Reitter, 1889

Search for bbbˉbˉbb\bar{b}\bar{b} tetraquark decays in 4 muons, B+B−B^{+} B^{-}, B0Bˉ0B^0 \bar{B}^0 and Bs0Bˉs0B_s^0 \bar{B}_s^0 channels at LHC

We perform a quantitative analysis of the $bb\bar{b}\bar{b}$ tetraquark decays into hidden- and open-bottom mesons and calculate, for the first time, the $bb\bar{b}\bar{b}$ tetraquark total decay width. On the basis of our results, we propose the $bb\bar{b}\bar{b} \to B^{+} B^{-} (B^0 \bar{B}^0) (B_s^0 \bar{B}_s^0) \to l^{+} l^{-}+\text{X}$ decays as the most suitable channels to observe the $bb\bar{b}\bar{b}$ tetraquark states, since the calculated two-lepton cross section upper limit, $\simeq 39$ fb, is so large as to be potentially detectable with the 2018 LHCb sensitivity, paving the way to the observation of the $bb\bar{b}\bar{b}$ tetraquark in the future LHCb upgrade. The $4\mu$ signal for the ground state, $J^{PC}=0^{++}$, is likely to be too small even for the upgraded LHCb, but it may not be hopeless for the $J^{PC}=2^{++}$ fully-bottom state.Comment: 11 pages, 1 figur

Copper microenvironments in the human body define patterns of copper adaptation in pathogenic bacteria

Copper is an essential micronutrient for most organisms that is required as a cofactor for crucial copper-dependent enzymes encoded by both prokaryotes and eukaryotes. Evidence accumulated over several decades has shown that copper plays important roles in the function of the mammalian immune system. Copper accumulates at sites of infection, including the gastrointestinal and respiratory tracts and in blood and urine, and its antibacterial toxicity is directly leveraged by phagocytic cells to kill pathogens. Copper-deficient animals are more susceptible to infection, whereas those fed copper-rich diets are more resistant. As a result, copper resistance genes are important virulence factors for bacterial pathogens, enabling them to detoxify the copper insult while maintaining copper supply to their essential cuproenzymes. Here, we describe the accumulated evidence for the varied roles of copper in the mammalian response to infections, demonstrating that this metal has numerous direct and indirect effects on immune function. We further illustrate the multifaceted response of pathogenic bacteria to the elevated copper concentrations that they experience when invading the host, describing both conserved and species-specific adaptations to copper toxicity. Together, these observations demonstrate the roles of copper at the host–pathogen interface and illustrate why bacterial copper detoxification systems can be viable targets for the future development of novel antibiotic drug development programs.</jats:p

A user’s guide to golden gate cloning methods and standards

The continual demand for specialized molecular cloning techniques that suit a broad range of applications has driven the development of many different cloning strategies. One method that has gained significant traction is Golden Gate assembly, which achieves hierarchical assembly of DNA parts by utilizing Type IIS restriction enzymes to produce user-specified sticky ends on cut DNA fragments. This technique has been modularized and standardized, and includes different subfamilies of methods, the most widely adopted of which are the MoClo and Golden Braid standards. Moreover, specialized toolboxes tailored to specific applications or organisms are also available. Still, the quantity and range of assembly methods can constitute a barrier to adoption for new users, and even experienced scientists might find it difficult to discern which tools are best suited toward their goals. In this review, we provide a beginner-friendly guide to Golden Gate assembly, compare the different available standards, and detail the specific features and quirks of commonly used toolboxes. We also provide an update on the state-of-the-art in Golden Gate technology, discussing recent advances and challenges to inform existing users and promote standard practices