3 research outputs found
In Vivo Subacute Toxicity and Antidiabetic Effect of Aqueous Extract of Nigella sativa
Context. Nigella sativa seeds are usually used as traditional medicine for a wide range of therapeutic purposes. Objective. To investigate the subacute toxicity of NS aqueous extract and select its lowest dose to study its antidiabetic effect. Methods. 5 AqE.NS doses (2, 6.4, 21, 33, and 60âg/Kg) were daily administered to mice by gavage. Biochemical parameters measurements and histological study of the liver and the kidney were performed after 6 weeks of supplementation. Thereafter, and after inducing diabetes by alloxan, rats were treated by 2âg/Kg of AqE.NS during 8 weeks. Metabolic parameters were measured on sera. A horizontal electrophoresis of plasmatic lipoprotein was conducted. Glycogen, total lipids, and triglycerides were measured in the liver. TBARS were evaluated on adipose tissue, liver, and pancreas. Results. AqE.NS showed no variation in urea and albumin at the 5 doses, but hepatotoxicity from 21âg/Kg was confirmed by histopathological observations of the liver. In diabetic rats, AqE.NS significantly decreased glycemia, TG, T-cholesterol, LDL-c, and TBARS and showed a restored insulinemia and a significant increase in HDL-c. Results on the liver indicated a decrease in lipids and a possible glycogenogenesis. Conclusion. AqE.NS showed its safety at low doses and its evident antihyperglycemic, antihyperlipidemic, and antioxidant effect
Effet thĂ©rapeutique de lâextrait aqueux de Globularia alypum suite Ă une insulinotoxicitĂ© induite in vitro sur les cardiomyocytes de Rattus norvegicus. [Therapeutic effect of aqueous extract of Globularia alypum following in vitro insulin induced toxicity on Rattus norvegicus cardiomyocytes]
Introduction. LâinsulinoârĂ©sistance, marquĂ©e par une rĂ©action inflammatoire via la production de TNFâα et par lâhyperinsulinĂ©mie compensatoire, conduit Ă long terme Ă lâinstallation dâune glucolipotoxicitĂ© et du diabĂšte de type 2. Objectif. Dans cette Ă©tude, les effets dâun extrait aqueux de Globularia alypum (Ga) a Ă©tĂ© analysĂ© in vitro, suite Ă un stress induit sur le cardiomyocyte de Rattus norvegicus. MatĂ©riel et MĂ©thodes. Le stress a Ă©tĂ© induit par addition dâune dose Ă©levĂ©e dâinsuline (10 UI/ mL). Les taux de prolifĂ©ration ont Ă©tĂ© dĂ©terminĂ©s par comptage et les contenus cellulaires en monoxyde dâazote (NO), en malondialdĂ©hyde (MDA) et en catalase ont Ă©tĂ© Ă©valuĂ©s. RĂ©sultats. En prĂ©sence de la dose Ă©levĂ©e dâinsuline, les cardiomyocytes ont montrĂ© une diminution de la survie cellulaire, un dĂ©sĂ©quilibre du statut redox en faveur dâune augmentation de NO et de MDA et dâune diminution dâune enzyme du systĂšme antioxydant, la catalase. Suite Ă lâaction de lâextrait de Ga, il a Ă©tĂ© notĂ© une amĂ©lioration du taux de prolifĂ©ration cellulaire, une diminution du taux de No et de MDA ainsi quâune augmentation de lâactivitĂ© de la catalase. Conclusion. LâĂ©tat de stress induit sur le cardiomyocyte par lâinsulinoârĂ©sistance, mimĂ© par la dose Ă©levĂ©e dâinsuline, entraĂźne des altĂ©rations physiologiques et biochimiques. Ces derniĂšres, prĂ©curseurs de mort cellulaire par apoptose sont attĂ©nuĂ©es aprĂšs addition dâun extrait de Globularia alypum, lequel pourrait constituer une cible thĂ©rapeutique en aval de lâinstallation du DT2
Glucotoxicity Induced Oxidative Stress and Inflammation In Vivo
Context. Brassica rapa is considered as natural source of antioxidants and is used to treat diabetes. Objective. Our study carried the impact of glucotoxicity induced in vivo and in vitro in vascular smooth muscle cells (VSMCs) in Psammomys and the therapeutic effect of Brassica rapa (AEBr). Materials and Methods. We administered a hyperglucidic diet (30% sucrose) for 9 months and a treatment for 20 days with AEBr at 100âmg/kg. VSMCs were submitted to D-Glucose (0.6%) for 48 hours and treated with AEBr (2100âÎŒg/mL) for 24 hours. We measured, in blood metabolic parameters, the redox statues and inflammatory markers in adipose tissue. Histological study was effectuated in liver. In VSMCs, we measured markers of glucotoxicity (IRS1p Serine, AKT) inflammation (NO, MCP1, TNFα, and NF-ÎșB) and oxidative stress (oxidants and antioxydants markers). Cell viability and apoptosis were estimated by the morphological study. Results. AEBr corrects the metabolic parameters and inflammatory and oxidative markers in blood and homogenate tissue and reduces lipid droplets in liver. It induces, in VSMCs, a significant decrease of IRS1p serine, cyt c, NO, MCP1, TNFα, NF-ÎșB, protein, and lipid oxidation and increases cell viability, AKT, ERK1/2, catalase, and SOD activity. Conclusion. Brassica enhanced the antidiabetic, anti-inflammatory, and antioxidant defense leading to the protection of cardiovascular diseases