A Study to Correlate Endometrial Hyperplasia with Ovarian Stromal Change

Background: Endometrial hyperplasia in peri-menopausal age group is frequently encountered as a result of unopposed excess estrogen action, exact source of which is still debatable. Possible source is ovarian stroma. There must be some subtle changes in ovarian stroma to produce excess estrogen in such states. This study was performed to provide new insights into the various patterns of subtle ovarian stromal changes and its relation to morphological alteration of endometrial glands and stroma.Methods: 62 women in peri-menopausal age group, diagnosed as endometrial hyperplasia on uterine curettage and unresponsive to conventional therapy, treated by total hysterectomy with unilateral/bilateral salpingo-oophorectomy, were chosen randomly. Serial step sections of endometrial biopsies were examined by routine microscopy. Endometrial hyperplasia was classified following the current WHO classification. The sections from the ovary were examined for changes in the ovarian stroma.Results: Out of the 62 total cases having endometrial hyperplasia, 53 cases (83.5%) had one or more subtle ovarian stromal changes and 9 cases (14.5%) lacked the ovarian stromal changes. Out of the 50 cases without endometrial hyperplasia, only eight had subtle ovarian changes. In the 53 cases, which showed ovarian stromal changes, the changes were found in combination and variable proportions.Conclusion: Ovarian stromal changes were significantly associated with endometrial hyperplasia

DEVELOPMENT OF QUALITY CONTROL PARAMETERS FOR STANDARDIZATION OF A NOVELMUCILAGE OBTAINED FROM OKRA (ABELMOSCHUS ESCULENTUS (L.) MOENCH) FRUIT

Objective: The main objective was to focus on qualitative and quantitative analysis of isolated okra mucilage by High-performance Thin Layer Chromatography to set up the quality control parameters for the isolated mucilage. Methods: High-performance thin-layer chromatography (HPTLC) were applied for the identification of components present in methanolic and ethanolic fruit extract of okra at 254 nm and 356 nm. Quantitative analysis of amino acid ascorbic acid and total polyphenol content was determined. Results: The results showed that the yield percentage for methanolic and ethanolic fruit extract of the okra fruit mucilage was found to be 13.5 and12.5% respectively. HPTLC determination of methanolic and ethanolic okra fruit extract showed the presence of 8 components with Rf values in the range of 0.14 to 0.62 and 0.14 to 0.54 respectively when detected at wavelengths 254 nm and at 356 nm. The total amino acid in okra fruit methanolic extract was found to be 11.45%w/w. The ascorbic acid content in methanolic okra fruit extract and ethanolic okra fruit extract was found to be 0.24 %w/w and 0.18% w/w respectively. The total phenolic contents (tannic acid equivalents, mg/g) in the methanolic and ethanolic okra fruit extracts were calculated to be 4.6 % w/wand 5.3% w/w respectively. Conclusion: The data revealed dual benefits like it can act as a potential novel functional ingredient with health-promoting application due to the presence of ascorbic acid and total phenolics contents and at the same time the data provided guidelines for quality control parameters for the isolated okra frui

Collapse/Flattening of Nucleonic Bags in Ultra-Strong Magnetic Field

It is shown explicitly using MIT bag model that in presence of ultra-strong magnetic fields, a nucleon either flattens or collapses in the direction transverse to the external magnetic field in the classical or quantum mechanical picture respectively. Which gives rise to some kind of mechanical instability. Alternatively, it is argued that the bag model of confinement may not be applicable in this strange situation.Comment: 8 pages, REVTEX, 3 figures .eps files (included

Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers

Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules. The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly l-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein. Despite many studies available with PLGA-based protein-loaded nanoparticles, production know-how, process parameters, protein loading, duration of protein release, narrowing polydispersity of particles have not been investigated enough to scale up manufacturing of protein-loaded nanoparticles in formulations. Different process parameters such as protein/polymer ratio, homogenizing speed during emulsifications, particle surface morphology and surface charges, particle size analysis and in-vitro protein release were investigated. The in-vitro protein release study suggests that release profile of BSA from nanoparticles could be modulated by changing protein-polymer ratios and/or by varying homogenizing speed during multiple-emulsion preparation technique. The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations. Decreased (10,000 rpm) or enhanced (24,000 rpm) homogenizing speeds resulted in increased polydispersion with larger particles having no better protein-loading and -release profiles in the present study

Chiral Properties of QCD Vacuum in Magnetars- A Nambu-Jona-Lasinio Model with Semi-Classical Approximation

The breaking of chiral symmetry of light quarks at zero temperature in presence of strong quantizing magnetic fiels is studied using Nambu-Jona-Lasinio (NJL) model with Thomas-Fermi type semi-classical formalism. It is found that the dynamically generated light quark mass can never become zero if the Landau levels are populated and the mass increases with the increase of magnetic field strength.Comment: REVTEX 11 Pages, One .eps figure (included