Effect of One Session of Resistance Exercises on Expression of BDNF Gene and TrkB Receptor in Alzheimer Model Male Wistar Rats

Background & Objective: This study is intended to evaluate the effect of One Session of resistance exercises on expression of BDNF gene and TrkB receptor in Alzheimer model male Wistar rats.  Materials & Methods: 32 mature male Wistar rats with mean weight of 230 to 280 grams were chosen and divided into Alzheimer and Sham groups. Rats in Sham group received normal saline while rats in Alzheimer group received STZ via intraventricular injection. These rats were then divided into the following four subgroups: 1. Resting Sham, 2. Exercising Sham, 3. Resting Alzheimer, and 4. Exercising Alzheimer. The two exercising rat subgroups, exercised 3 times a week for a period of 8 weeks. Exercise included lifting weight from the ladder. At the end of 8th week and 24 hours after last exercise session, the rats scarified by head separation. Hippocampus tissue was precisely extracted and samples were sent to laboratory for molecular and cellular tests. To determine the gene expression, RT-PCR method was used for analyzing the data and ANOVA was used. Results: The amount of BDNF, and TrkB between exercising rats and resting rats. These amounts were much higher in exercising Alzheimer rats group. Conclusion: It seems that eight weeks of resistance exercises increased expression of BDNF gene and TrkA an TrkB receptor in Alzheimer model Wistar rats

Effects of Eight Weeks of Resistance Exercises on Neurotrophins and Trk Receptors in Alzheimer Model Male Wistar Rats

Introduction: This study evaluates the effects of 8 weeks of resistance exercises on the expression of neurotrophins and Trk receptors in Alzheimer model male Wistar rats.  Methods: For this purpose, 32 mature male Wistar rats with a mean weight of 230-280 g were chosen and divided into Alzheimer and Sham groups. The rats in the sham group received normal saline, while the ones in the Alzheimer group received streptomycin via intraventricular injection. These rats were then divided into the following four subgroups: 1) resting sham, 2) exercising sham, 3) resting Alzheimer, and 4) exercising Alzheimer. The two exercising rat subgroups exercised three times a week for 8 weeks. A weight was attached to their tails, and they had to carry it on a 26-step ladder in each cycle. Resting groups were handled every day to minimize the effects of stress. At the end of the eighth week and 24 hours after the last exercise session (to avoid the effects of the last exercise session), the rats were put under deep anesthesia and beheaded. Hippocampus tissues were precisely extracted, and samples were sent to the laboratory for molecular and cellular tests. To investigate gene expression, quantitative RT-PCR was used. Results: The tests for comparing the mean values of BDNF, NT3, NGF, TrkA, and TrkB in two rat groups showed that with error levels of less than 5%, there is a significant difference in the amounts of BDNF, NT3, NGF, TrkA, and TrkB between exercising rats and resting ones. These amounts were much higher in the exercising Alzheimer rats group. Conclusion: Eight weeks of resistance exercises increased the expression of BDNF, NT3, and NGF genes and TrkA and TrkB receptors in Alzheimer model Wistar rats