ECOLOGICAL RESEARCH ON THE DYNAMICS OF BIOMASS AND BIOLOGICAL PRODUCTIVITY SPECIES OCTOLASION LACTEUM “(OLIGOCHAETA – LUMBRICIDAE)”

The present study analyzes Octolasion (Oligochaeta - Lumbricidae) and Octolasion lacteum species in terms of biomass dynamics and biological productivity at different soil levels (from 10cm to 40cm) in three hilly forest areas of Cândeşti Piedmont, Arges County, in the period March- October 2008. This species belongs to the group of endogeic species with an important role in mixing organic-mineral soils and is considered eudominant with the most important role in the functioning of forest ecosystems. The results showed that the monthly biomass of the soil levels in the three areas ranged from 0.57 - 3.221 mg.d.s/m2 in the deciduous forest, 0.22 - 0.492 mg.d.s/m2 in the spruce forest and 0.67 - 2,389 mg.d.s/m2 in the grassland. The deciduous (20.155 mg.su./m2) and the grassland forests (22.643 mg.su./m2) had the highest biological productivity

THE SPECIFIC STRUCTURE, FUNCTIONAL ROLE AND ORGANISATION OF LUMBRICIDAE IN A SECONDARY NATURAL GRASSLAND

The present work analyses the indices of aggregation, dispersion, expansion, dominance and receptivity of lumbricidae fauna in a grassland located in the high hills of Piedmont Candesti in the south-east of Arges County. The higher aggregation of species in the grassland, in certain months expressed a lower number of points with optimal food and physiological conditions. It also emerges the idea that, a higher density of the population caused a higher degree of expansion, which gave the species a determining role within the biogenesis

ALUMINUM EFFECTS IN ALLEATO 80 WG FUNGICIDE ON EISENIA FOETIDA SPECIES

The study highlights the influence of Alleato 80WG used in the laboratory chronic test, on an artificial soil according to OECD, on Eisenia foetida species, in terms of survival rate, biomass and content of bioaccumulated aluminum.50 mature individuals were used for each concentration in 5 repetitions (10 individuals / repetition). The toxic concentrations used were: LC50= 500 mg•Kg-1(V1); LC50= 400 mg•Kg-1(V2); LC50= 300 mg•Kg-1(V3); LC50= 200 mg•Kg-1(V4)/ dry soil, for 5 samples, the 5th (V5) being the control sample. The toxic concentrations in V1; V2 and V3, caused a significant decrease of the survival rate. The average values were 80%, as compared to V4 and control sample (V5). The correlations between Alleato 80 WG and the survival rate were negative (p<0.0001) while the increase of toxic concentration caused the decrease of the survival rate. After being subjected to toxic Alleato 80WG, biomass recorded for individual variants V1, V2, V3, and V4 does not differ significantly from version V5. The increase of toxic concentrations caused a significant increase of aluminum bioaccumulated by earthworms (R Square=0.304; p=0.004)

CONTRIBUTIONS TO THE STUDY ON THE EFFECTS OF STOMP 330 EC HERBICIDE UPON THE MARSH FROG

Pesticides are the best examples of risky compounds because they spread all over the environment. The experiments were performed to determine the effect of the Stomp 330EC herbicide at concentrations of 0.5 x 103, 1 x 103, 2 x 103 and respectively 4 x10 3 ml of herbicide/l of water on Pelophylax ridibundus tadpoles and adults. The herbicide produces delay and reduction of hatching (at the concentration of 0.004 ml/l), slowing of growth in volume and length as well as decreased oxygen consumption and survival of the tadpoles. The frequency of buco-pharingeal movements in adults of marsh frog is influenced by the Stomp® 330 EC herbicide at a concentration of 0.002 and 0.004 ml/l of water. After 14 days of exposure to the herbicide, the average number of erythrocytes increases, the average number of leukocytes decreases and the level of glucose changes

BIOACCUMULATION AND EFFECTS OF Zn AND Mn CONTAINED BY DITHANE M45 ON EISENIA FOETIDA (OLIGOCHAETA-LUMBRICIDAE) SPECIES

The study assesses the effects of Zn and Mn contained by Dithane M45 fungicide on Eisenia foetida epigenous species. There were five experimental variants of five repetitions each. Dithane M45 concentrations were: V1:250mgkg-1/dry soil, V2:200mgkg-1/dry soil, V3:150mgkg-1/ dry soil, V4:100mgkg-1/dry soil, with the fifth (V5) as control variant. There were analyzed: 1. survival rate (%) after applying toxic and correlations between survival rate and toxic concentrations; 2. initial biomass (g) and biomass resulting from (g) intoxication; 3. Zn and Mn bioaccumulation (ppm) in earthworms. Conclusions: the survival rate decreased to the greatest concentrations compared to control variant; individual biomass decreased after applying toxic; Mn and Zn bioaccumulation in earthworms increased with increasing concentration of Dithane M45

STUDY REGARDING THE INFLUENCE OF ROYAL INSECTICIDE ON SOME PHYSIOLOGICAL INDEXES AT CARASSIUS AURATUS GIBELIO BLOCH L. 1758

The main objective of this study is to see how the royal insecticide influences the energetic metabolism, the breathing rhythm, the blood sugar and the number of red blood cells at the Prussian carp individuals. Experimental samples were subjected to under-lethal concentrations of 0.00125, 0.0025 and 0.005 ml/l royal insecticide from 24 to 336 hours. The physiologic parameter with the highest growth rate was the oxygen consumption, which, at the concentration of 0.005 ml/l grew by 50.81% in 168 hours, compared to the witness values, registering the value of 216.66 ml oxygen/kilo/hour compared to 143.66 oxygen ml/kilo/hour. The blood sugar was the single physiological biomarker which decreased, so that the lowest value was 48 mg/dl, 29.62% approximately lower compared to the witness value (68.2 mg/dl) at a concentration of 0.00125 ml/l