Antibacterial activity of Zinc Oxide nanoparticles synthesized by the green method from Hyssopus officinail extract

Background: In recent years, in order to prevent the excessive use of antibiotics, researchers are paying more attention to nanoparticles with antibacterial properties. The aim of this study was to evaluate the antibacterial activity of Zinc Oxide nanoparticles (ZnO-NPs) synthesized by the green method from the extract of the Hyssopus officinail against some of the gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis) and gram-negative (Pseudomonas aeruginosa, Escherichia coli). Materials and Methods: For this experimental study, dry powder was prepared from Hyssopus officinail and Zinc acetate was used as a Zinc source. After synthesizing the nanoparticle via green method according to the protocol, concentrations of 250, 500, 1000 and 2000 µg/ml were prepared. The blanc discs with above mentioned concetrations were placed on the plate of bacteria cultured on Muller Hinton Agar culture media. Antibiotic disc gentamicin was used as control. Results: The results showed that ZnO-NPs synthesized from the Hyssopus officinail extract at concentrations of 1000 and 2000 μg/ml had a proper antimicrobial activity, so that the inhibition in the concentration of 2000 μg/ml in E. coli and S. aureus were almost equal to the gentamicin. Conclusion: The results obtained from antibacterial property of ZnO-NPs synthesized from the Hyssopus officinail extract showed that there is a direct correlation between the concentration of nanoparticles and the elimination of bacteri

Investigating the Cytotoxic Effect of Urolithin B and Cerium Oxide Nanoparticles Synthesized from Aloe Vera Plant on Brain Cancer Cell Colony (U87MG)

Background: Brain cancer is one of the types of cancer that appears with the growth of cancer cells in the brain. In this experimental study, we investigated and compared the effect of cerium oxide nanoparticles synthesized by the green method from Aloe vera with urolithin B on U87MG cancer cells. Materials and Methods: Cerium oxide nanoparticles were produced by the green synthesis method from the Aloe vera plant and confirmed by nanoparticle size characterization tests and an FESEM microscope. The U87MG cells were obtained from Pasteur Institute of Tehran, Iran, and after passage, they were treated with cerium oxide nanoparticles and urolithin B drug for 72 h and cell colonies for 15 days. Results: The IC50 of cancer cells in the MTT test for urolithin B and cerium oxide nanoparticles synthesized by green method from Aloe vera plant with urolithin B were 170 μM and 135 μM, respectively. In addition, the survival percentage results from the clonogenic test at a concentration of 30 μM for cerium oxide nanoparticles (78%), urolithin B(61%), and cerium oxide nanoparticles with urolithin B(30%), and for a concentration of 60 μM, it was 60, 42, and 16%, respectively. Conclusion: Urolithin B has a higher cytotoxic effect (170μM) than cerium oxide nanoparticles synthesized by the green method from the Aloe vera plant, and when combined with cerium oxide nanoparticles, this cytotoxic effect increased more (135μM). It also reduced the survival percentage of colonies within 15 days of treatment