Biochemical and Morphological Studies of Rat Submandibular Gland: III. Effects of Testosterone Treatment on Proteins of Granule-Rich Fraction

Mature adult male rats and castrated and testosterone-treated castrated adult rats were injected with pilocarpine HGL and 3-H-lysine and sacrificed sequentially over an eight-hour time period. Following homogenization and differential centrifugation, three subcellular fractions from each group of animals were analyzed by gel electrophoresis and liquid scintillation. Two proteins in the granule-rich fraction appeared in larger amounts on the densitometric scans and appeared to represent a larger proportion of newly synthesized proteins than the other proteins.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66712/2/10.1177_00220345770560030901.pd