A detailed view into the eruption clouds of Santiaguito volcano, Guatemala, using Doppler radar

Using Doppler radar technology we are able to show that eruptions at Santiaguito volcano, Guatemala, are comprised of multiple explosive degassing pulses occurring at a frequency of 0.2 to 0.3 Hz. The Doppler radar system was installed about 2.7 km away from the active dome on the top of Santa Maria volcano. During four days of continuous measurement 157 eruptive events were recorded. The Doppler radar data reveals a vertical uplift of the dome surface of about 50 cm immediately prior to a first degassing pulse. Particle velocities range from 10 to 15 m/s (in the line of sight of the radar). In 80% of the observed eruptions a second degassing pulse emanates from the dome with significantly higher particle velocities (20-25 m/s again line of sight) and increased echo power, which implies an increase in mass flux. We carry out numerical experiments of ballistic particle transport and calculate corresponding synthetic radar signals. These calculations show that the observations are consistent with a pulsed release of material from the dome of Santiaguito volcano

Evaluating the impact of government energy R&D investments through a multi-attribute utility-based decision tool

Thesis (S.M. in Engineering and Management)--Massachusetts Institute of Technology, Engineering Systems Division, System Design and Management Program, 2011.Cataloged from PDF version of thesis.Includes bibliographical references (p. 101-104).Government agencies characteristically face dynamic policy and investment environments yet frequently rely on ad-hoc decision-making methods in response to complexities inherent in their operating landscape. Additionally, standard decision making methods typically undervalue projects by ignoring difficult to value, non-monetary benefits. This presents a problem for public institutions, such as the Department of Energy (DOE), where goals relating to the environment and national security are difficult to quantify. As a result, it is especially challenging to accurately optimize the use of public funds. The Department of Energy (DOE) is responsible for making significant investment decisions under extreme uncertainty with respect to the nation's public energy portfolio. Recently, leaders internal and external to the government have called for a comprehensive and structured approach to assess the DOE's portfolio of programs and initiatives (PCAST, 2010), (American Energy Innovation Council, 2011). Given the broad spectrum of the DOE's current portfolio, from basic R&D to demonstration and across every major energy technology, evaluating the impacts of its potential investments is complex. Within the Department of Energy's Planning Analysis and Evaluation (PA&E) team, a proposal was made to develop a first-of-a-kind decision tool that would provide rigorous analysis of cost and benefit trade-offs associated with the DOE's investments. The decision tool was designed to couple a state-of-the-art climate and energy model with sophisticated multi-attribute-based decision methods. The research described in this thesis illuminates the advantages and shortcomings of the initial decision tool structure, and presents a second generation model that is tailored to the DOE's operational context. Finally, in order to expand its use for long-range strategy formation, an evolution of this second generation model is explored through the application of recent theoretical methodologies. The resulting decision tool is intended to play an informative role within a comprehensive portfolio review by enabling the enumeration of budgetary trade-offs that address high-level, strategy questions facing the DOE.by Kacy J. Gerst.S.M.in Engineering and Managemen

Genetic and biochemical analysis of the adenylyl cyclase-associated protein, cap, in Schizosaccharomyces pombe

We have identified, cloned, and studied a gene, cap, encoding a protein that is associated with adenylyl cyclase in the fission yeast Schizosaccharomyces pombe. This protein shares significant sequence homology with the adenylyl cyclase-associated CAP protein in the yeast Saccharomyces cerevisiae. CAP is a bifunctional protein; the N-terminal domain appears to be involved in cellular responsiveness to RAS, whereas loss of the C-terminal portion is associated with morphological and nutritional defects. S. pombe cap can suppress phenotypes associated with deletion of the C-terminal CAP domain in S. cerevisiae but does not suppress phenotypes associated with deletion of the N-terminal domain. Analysis of cap disruptants also mapped the function of cap to two domains. The functional loss of the C-terminal region of S. pombe cap results in abnormal cellular morphology, slow growth, and failure to grow at 37-degrees-C. Increases in mating and sporulation were observed when the entire gene was disrupted. Overproduction of both cap and adenylyl cyclase results in highly elongated large cells that are sterile and have measurably higher levels of adenylyl cyclase activity. Our results indicate that cap is required for the proper function of S. pombe adenylyl cyclase but that the C-terminal domain of cap has other functions that are shared with the C-terminal domain of S. cerevisiae CAP

The First Second of Volcanic Eruptions from the Erebus Volcano Lava Lake, Antarctica—Energies, Pressures, Seismology, and Infrasound

[1] We describe a multiparameter experiment at Erebus volcano, Antarctica, employing Doppler radar, video, acoustic, and seismic observations to estimate the detailed energy budget of large (up to 40 m-diameter) bubble bursts from a persistent phonolite lava lake. These explosions are readily studied from the crater rim at ranges of less than 500 m and present an ideal opportunity to constrain the dynamics and mechanism of magmatic bubble bursts that can drive Strombolian and Hawaiian eruptions. We estimate the energy budget of the first second of a typical Erebus explosion as a function of time and energy type. We constrain gas pressures and forces using an analytic model for the expansion of a gas bubble above a conduit that incorporates conduit geometry and magma and gas parameters. The model, consistent with video and radar observations, invokes a spherical bulging surface with a base diameter equal to that of the lava lake. The model has no ad hoc free parameters, and geometrical calculations predict zenith height, velocity, and acceleration during shell expansion. During explosions, the energy contained in hot overpressured gas bubbles is freed and partitioned into other energy types, where by far the greatest nonthermal energy component is the kinetic and gravitational potential energy of the accelerated magma shell (\u3e109 J). Seismic source energy created by explosions is estimated from radar measurements and is consistent with source energy determined from seismic observations. For the generation of the infrasonic signal, a dual mechanism incorporating a terminally disrupted slug is proposed, which clarifies previous models and provides good fits to observed infrasonic pressures. A new and straightforward method is presented for determining gas volumes from slug explosions at volcanoes from remote infrasound recordings

Cloud Photogrammetry from Space

The most commonly used method for satellite cloud top height (CTH) compares brightness temperature of the cloud with the atmospheric temperature profile. Because of the uncertainties of this method, we propose a photogrammetric approach. As clouds can move with high velocities, even instruments with multiple cameras are not appropriate for accurate CTH estimation. Here we present two solutions. The first is based on the parallax between data retrieved from geostationary (SEVIRI, HRV band; 1000 m spatial resolution) and polar orbiting satellites (MODIS, band 1; 250 m spatial resolution). The procedure works well if the data from both satellites are retrieved nearly simultaneously. However, MODIS does not retrieve the data at exactly the same time as SEVIRI. To compensate for advection in the atmosphere we use two sequential SEVIRI images (one before and one after the MODIS retrieval) and interpolate the cloud position from SEVIRI data to the time of MODIS retrieval. CTH is then estimated by intersection of corresponding lines-of-view from MODIS and interpolated SEVIRI data. The second method is based on NASA program Crew Earth observations from the International Space Station (ISS). The ISS has a lower orbit than most operational satellites, resulting in a shorter minimal time between two images, which is needed to produce a suitable parallax. In addition, images made by the ISS crew are taken by a full frame sensor and not a push broom scanner that most operational satellites use. Such data make it possible to observe also short time evolution of clouds

Evolution of E2 transition strength in deformed hafnium isotopes from new measurements on 172^{172}Hf, 174^{174}Hf, and 176^{176}Hf

The available data for E2 transition strengths in the region between neutron-deficient Hf and Pt isotopes are far from complete. More and precise data are needed to enhance the picture of structure evolution in this region and to test state-of-the-art nuclear models. In a simple model, the maximum collectivity is expected at the middle of the major shell. However, for actual nuclei, this picture may no longer be the case, and one should use a more realistic nuclear-structure model. We address this point by studying the spectroscopy of Hf. We remeasure the 2^+_1 half-lives of 172,174,176Hf, for which there is some disagreement in the literature. The main goal is to measure, for the first time, the half-lives of higher-lying states of the rotational band. The new results are compared to a theoretical calculation for absolute transition strengths. The half-lives were measured using \gamma-\gamma and conversion-electron-\gamma delayed coincidences with the fast timing method. For the determination of half-lives in the picosecond region, the generalized centroid difference method was applied. For the theoretical calculation of the spectroscopic properties, the interacting boson model is employed, whose Hamiltonian is determined based on microscopic energy-density functional calculations. The measured 2^+_1 half-lives disagree with results from earlier \gamma-\gamma fast timing measurements, but are in agreement with data from Coulomb excitation experiments and other methods. Half-lives of the 4^+_1 and 6^+_1 states were measured, as well as a lower limit for the 8^+_1 states. We show the importance of the mass-dependence of effective boson charge in the description of E2 transition rates in chains of nuclei. It encourages further studies of the microscopic origin of this mass dependence. New data on transition rates in nuclei from neighboring isotopic chains could support these studies.Comment: 16 pages, 16 figures, 7 tables; Abstract shortened due to character limi

Negative regulation of syntaxin4/SNAP-23/VAMP2-mediated membrane fusion by Munc18c <i>In Vitro</i>

Background: Translocation of the facilitative glucose transporter GLUT4 from an intracellular store to the plasma membrane is responsible for the increased rate of glucose transport into fat and muscle cells in response to insulin. This represents a specialised form of regulated membrane trafficking. Intracellular membrane traffic is subject to multiple levels of regulation by conserved families of proteins in all eukaryotic cells. Notably, all intracellular fusion events require SNARE proteins and Sec1p/Munc18 family members. Fusion of GLUT4-containing vesicles with the plasma membrane of insulin-sensitive cells involves the SM protein Munc18c, and is regulated by the formation of syntaxin 4/SNAP23/VAMP2 SNARE complexes. Methodology/Principal Findings Here we have used biochemical approaches to characterise the interaction(s) of Munc18c with its cognate SNARE proteins and to examine the role of Munc18c in regulating liposome fusion catalysed by syntaxin 4/SNAP23/VAMP2 SNARE complex formation. We demonstrate that Munc18c makes contacts with both t- and v-SNARE proteins of this complex, and directly inhibits bilayer fusion mediated by the syntaxin 4/SNAP23/VAMP2 SNARE complex. Conclusion/Significance Our reductionist approach has enabled us to ascertain a direct inhibitory role for Munc18c in regulating membrane fusion mediated by syntaxin 4/SNAP23/VAMP2 SNARE complex formation. It is important to note that two different SM proteins have recently been shown to stimulate liposome fusion mediated by their cognate SNARE complexes. Given the structural similarities between SM proteins, it seems unlikely that different members of this family perform opposing regulatory functions. Hence, our findings indicate that Munc18c requires a further level of regulation in order to stimulate SNARE-mediated membrane fusion

Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells

Diabetes is a global health problem caused primarily by the inability of pancreatic β-cells to secrete adequate levels of insulin. The molecular mechanisms underlying the progressive failure of β-cells to respond to glucose in type-2 diabetes remain unresolved. Using a combination of transcriptomics and proteomics, we find significant dysregulation of major metabolic pathways in islets of diabetic βV59M mice, a non-obese, eulipidaemic diabetes model. Multiple genes/proteins involved in glycolysis/gluconeogenesis are upregulated, whereas those involved in oxidative phosphorylation are downregulated. In isolated islets, glucose-induced increases in NADH and ATP are impaired and both oxidative and glycolytic glucose metabolism are reduced. INS-1 β-cells cultured chronically at high glucose show similar changes in protein expression and reduced glucose-stimulated oxygen consumption: targeted metabolomics reveals impaired metabolism. These data indicate hyperglycaemia induces metabolic changes in β-cells that markedly reduce mitochondrial metabolism and ATP synthesis. We propose this underlies the progressive failure of β-cells in diabetes.Peer reviewe