Evaluation of inorganic arsenic species in drinking water of the southeastern of the Buenos Aires province, Argentina

Arsenic in natural water is a worldwide concern due to chronic health effects in people exposed through the drinking water. The aim was to evaluate the distribution of As(III) and As(V), through ASV, in an Argentine area with low or absent information about these contaminant. The species of inorganic arsenic were quantified in groundwater, surface and drinking water, by Anodic Stripping Voltammetry (ASV) using a gold disc electrode. Total As was analyzed after the reduction step, calculated As(V) by difference. In some sites, total As values exceed the maximum limit for human consumption (10 µg/l), but the As(III)/As(V) ratio was significantly low. The lowest levels of total As were found in those sites related to possible anthropogenic activities, indicating that the content of it in the studied water is coming from natural environment. This study constitutes the first report of inorganic As speciation for the southeastern of Buenos Aires province.Fil: Robles, A. D. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras. Departamento de Ciencias Marinas. Toxicología Ambiental; Argentina.Fil: Robles, A. D. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Cohen, M. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras. Departamento de Ciencias Marinas. Toxicología Ambiental; Argentina.Fil: Romero, M. B. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras. Departamento de Ciencias Marinas. Toxicología Ambiental; Argentina.Fil: Romero, M. B. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Garay, F. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Físico Química; Argentina.Fil: Garay, F. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Bioquímica de Córdoba; Argentina.Fil: Gerpe, M. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras. Departamento de Ciencias Marinas. Toxicología Ambiental; Argentina.Fil: Gerpe, M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Química Analític

Preliminary study of total arsenic in a pampean lagoon with horticultural activity

El objetivo de la presente investigación fue evaluar la adsorción de arsénico en sedimentos de la laguna de los Padres en relación a aquellos parámetros que pueden influenciar su movilidad; proponiéndose este estudio como punto de partida para el análisis posterior de posibles aportes externos a esta cuenca. Se tomaron muestras de sedimentos y de agua en la laguna y su cuenca superficial. Las concentraciones de arsénico, hierro y manganeso fueron determinadas por espectroscopia de absorción atómica y de plasma inductivamente acoplado. Las concentraciones de As en sedimentos (5,8 -13,5 mg/kg) fueron significativamente superiores a las encontradas en agua (13,8-18,0 ug/L), evidenciando una baja movilidad. Se encontraron correlaciones significativamente positivas entre las concentraciones de arsénico y hierro, no así con manganeso. Estos resultados sugieren la relación entre ambos metales, provocando esa baja movilidad y la partición favorecida hacia los sedimentos.Centro de Investigaciones Geológica

Hypoxia compromises the mitochondrial metabolism of Alzheimer’s disease microglia via HIF1

Genetic Alzheimer’s disease (AD) risk factors associate with reduced defensive amyloid β plaque-associated microglia (AβAM), but the contribution of modifiable AD risk factors to microglial dysfunction is unknown. In AD mouse models, we observe con- comitant activation of the hypoxia-inducible factor 1 (HIF1) pathway and transcription of mitochondrial-related genes in AβAM, and elongation of mitochondria, a cellular response to maintain aerobic respiration under low nutrient and oxygen conditions. Overactivation of HIF1 induces microglial quiescence in cellulo, with lower mitochondrial respiration and proliferation. In vivo, overstabilization of HIF1, either genetically or by exposure to systemic hypoxia, reduces AβAM clustering and proliferation and increases Aβ neuropathology. In the human AD hippocampus, upregulation of HIF1α and HIF1 target genes correlates with reduced Aβ plaque microglial coverage and an increase of Aβ plaque-associated neuropathology. Thus, hypoxia (a modifiable AD risk factor) hijacks microglial mitochondrial metabolism and converges with genetic susceptibility to cause AD microglial dysfunction

Hypoxia compromises the mitochondrial metabolism of Alzheimer’s disease microglia via HIF1

Genetic Alzheimer’s disease (AD) risk factors associate with reduced defensive amyloid β plaque-associated microglia (AβAM), but the contribution of modifiable AD risk factors to microglial dysfunction is unknown. In AD mouse models, we observe concomitant activation of the hypoxia-inducible factor 1 (HIF1) pathway and transcription of mitochondrial-related genes in AβAM, and elongation of mitochondria, a cellular response to maintain aerobic respiration under low nutrient and oxygen conditions. Overactivation of HIF1 induces microglial quiescence in cellulo, with lower mitochondrial respiration and proliferation. In vivo, overstabilization of HIF1, either genetically or by exposure to systemic hypoxia, reduces AβAM clustering and proliferation and increases Aβ neuropathology. In the human AD hippocampus, upregulation of HIF1α and HIF1 target genes correlates with reduced Aβ plaque microglial coverage and an increase of Aβ plaque-associated neuropathology. Thus, hypoxia (a modifiable AD risk factor) hijacks microglial mitochondrial metabolism and converges with genetic susceptibility to cause AD microglial dysfunction.R.M.-D. was the recipient of a Sara Borrell fellowship from Instituto de Salud Carlos III (ISCIII) (CD09/0007). N.L.-U., C.O.-d.S.L., C.R.-M. and M.I.A.-V. were the recipients of FPU fellowships from Spanish Ministry of Education, Culture and Sport (FPU14/02115, AP2010‐1598, FPU16/02050 and FPU15/02898, respectively). A.H.-G. was the recipient of an FPI fellowship from the Spanish Ministry of Education, Culture and Sport (BES-2010-033886). This work was supported by grants from the Spanish MINEICO, ISCIII and FEDER (European Union) (SAF2012‐33816, SAF2015‐64111‐R, SAF2017-90794-REDT and PIE13/0004 to A.P.); by the Regional Government of Andalusia co-funded by CEC and FEDER funds (European Union) (‘Proyectos de Excelencia’; P12‐CTS‐2138 and P12‐CTS‐2232 to A.P.); by the ‘Ayuda de Biomedicina 2018’, Fundación Domingo Martínez (to A.P.) ; by the ISCIII of Spain, co-financed by FEDER funds (European Union) through grants PI18/01556 (to J.V.) and PI18/01557 (to A. Gutierrez); by Junta de Andalucía, co-financed by FEDER funds (grants UMA18-FEDERJA-211 (to A. Gutierrez) and US‐1262734 (to J.V.)); and by Spanish MINEICO (BFU2016-76872-R and BES-2011-047721 to E.B.).Peer reviewe

Assessment of Heavy Metal Accumulation in Two Aquatic Macrophytes: a Field Study

In order to assess the potential of two autochthonous plant species of Argentina as bioindicator and/or fitoremediator of metals, the accumulation of zinc, copper, cadmium and mercury in Schoenoplectus californicus and Ricciocarpus natans was studied. Total and labile contents of surface (SS) and near root sediments (NRS) were analyzed. Labile contents in NRS were lower than in SS, which was associated to the presence of S. californicus. Copper and zinc were positively correlated with total organic matter (TOM) whereas cadmium was not, suggesting low affinity of Cd to TOM. S. californicus presented the same pattern of distribution of those found in sediments (Zn > Cu > Cd). Bioaccumulation factors were close to (cadmium) or higher (zinc) than 1, while it was not conclusive for copper, revealing the S. californicus behaviour as an accumulator for zinc and indicator for cadmium. R. natans presented the highest metal levels among all studied matrix, with the presence of mercury. These findings showed the capacity of both S. californicus and R. natans to accumulate and remove heavy metals from sediments and water, which might be useful for phytoremediation programs. Keywords: aquatic macrophytes, S. californicus, R. natans, heavy metals, phytoremediation, bioindicator

Hematology and serum chemistry of female South American fur seals (Arctocephalus australis) from Lobos Island, Uruguay

Hematology and serum chemistry are used to diagnose disease in marine mammals incaptivity and in the wild. Clinicopathologic variables are the first step in determiningtreatment and assessing the suitability for rehabilitation and release. The South Americanfur seal (SAFS; Arctocephalus australis) occurs along the Pacific coast from southern Peruto Cape Horn and northward to southern Brazil on the Atlantic coast. The goal of this studywas to evaluate hematologic, clinical chemistry, and physical parameters of eight femaleSAFS from coastal Uruguay. Segmented neutrophils were the major leukocytes followedby lymphocytes. The mean aspartate aminotransferase (AST) was similar to that reportedpreviously for females and pups, except two females that showed higher levels. Alanineaminotransferase (ALT) in SAFS from our study was markedly higher than values reportedfor Chilean SAFS. AST and ALT values found could be related to liver damage. ALPlevels in the females were within the range reported previously. Creatinine was highercompared with females and pups from Chile. The differences found in hematology andserum chemistry between Chilean and Uruguayan populations may also be related todifferences in physiological and immune status. Basophils, myelocytes, metamyelocytes,lymphocyte plasma cells, and immature or pathologic forms were not observed. No unusualvalues were observed for red blood cells (RBC), white blood cells (WBC), and platelets.Glucose, uricemia, creatine kinase (CK), lactate dehydrogenase (LDH), Na, Cl, Ca, P and Klevels are the first report for the species. These are the first results on the hemotology andblood chemistry of otherwise wild SAFS from Isla de Lobos, which can be used to assessthe health of wild populations and during rehabilitation.Fil: Polizzi, Paula Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Fares Taie, S.. No especifíca;Fil: Romero, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Ponce de León, A. No especifíca;Fil: Davis, R.W.. Texas A&M University; Estados UnidosFil: Dolagaratz Carricavur, Arantxa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Chiodi Boudet, Leila Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Rodriguez, Diego Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Gerpe, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentin

Cadmium, zinc and copper accumulation in the squid Illex argentinus from the Southwest Atlantic Ocean

The levels and burdens of Cd, Zn, Cu and Hg were measured in the dorsal mantle, digestive gland and gonads of the squid Illex argentinus, from the Southwest Atlantic Ocean. Mature and immature individuals of both sexes were analysed. Correlations of heavy metal concentrations and burdens with sex and food habits were studied. The highest metal levels were found, particularly for Cd, in the digestive gland, which attained a concentration of up to 270 μg g−1 (wet weight). Dorsal mantle and gonads exhibited the same order of metal enrichment: Zn>Cu>Cd. Zinc and Cu levels were higher in ovaries than in testes and varied with the stage of maturation. The dorsal mantle exhibited the lowest heavy metal concentrations. Mercury levels were below the detection limit of the method in all the tissues analysed

Identification of molecular mechanisms related to nonthyroidal illness syndrome in skeletal muscle and adipose tissue from patients with septic shock

Objective Septic shock is one of various causes of nonthyroidal illness syndrome (NTIS). In humans, the molecular mechanisms involved in NTIS are mostly unknown. The aim of this study was to investigate, in patients with NTIS secondary to septic shock, changes in the expression of genes involved in the actions of thyroid hormones and in the activity of deiodinase enzymes, in two tissues important for protein and energy metabolism, skeletal muscle (SM) and subcutaneous adipose tissue (SAT). Design Hospitalized patients were divided into a control and a septic shock NTIS group. Measurement Serum collection for biochemical measurements, and SM and SAT biopsies for mRNA expression analysis of thyroid hormone receptors (THRB1, THRA1), retinoid X receptors (RXRA, RXRB, RXRG), nuclear receptor corepressor (NCOR1), silencing mediator of retinoid and thyroid hormone receptor (SMRT), steroid receptor coactivator (SRC1), type 1 and 2 deiodinases (D1, D2), monocarboxylate transporter 8 (MCT8), SECIS binding protein 2 (SBP2) and uncoupling protein 3 (UCP3) as well as D1, D2 and D3 enzyme activity measurements. Results The NTIS group had lower serum TSH, and free T3 and higher rT3 than controls. D1 and D3 were detected in SAT, with no differences found between the two groups; SM had very low D2 activity and again no differences were found between groups; D3 activity in SM was higher in NTIS than controls. SM expression of THRB1, RXRG and D2 was lower and RXRA higher in NTIS than controls. SAT from NTIS patients had lower MCT8, THRB1, THRA1, RXRG and SMRT, and higher UCP3 expression than controls. Conclusions In patients with septic shock NTIS tissue responses are orientated to decrease production and increase degradation (muscle) or decrease uptake (adipose tissue) of T3, as well as to decrease thyroid hormone actions