7 research outputs found
Higher-order reflection derivative spectrophotometry for non-destructive investigations of chlorophylls in green plants
No full textUV higher-order derivative spectrophotometry for the determination of the protein content of immobilized enzymes
No full textEinfluß des Drucks auf immobilisierte Enzyme
No full textPressure shows a distinct effect on the activity and stability of enzymes in solution. We
observed for Trypsin an increase of activity of 39,8 % at 55 °C. The investigations were
made continously by using a tubular reactor. Enzymes. immobilized on Eupergit C®, which is
a water-insoluble carrier, behave in a similar manner but with smaller influence of pressure. In opposite to Trypsin in solution, the carrierbound enzyme had a denaturation point of
60 °C (Trypsin in solution: 50 °C). Also the stability rose with pressure. Similar results
were obtained using immobilized Penicillin-Acylase. At 40°C and 300 bar no decrease of
activity could be observed after an incubation time of 2 hours. This high stability leads to to
an increased lifetime of the catalyst which is promotive for technical use
