93 research outputs found
Genome-Wide Identification of Transcriptional Start Sites in the Plant Pathogen Pseudomonas syringae pv. tomato str. DC3000
RNA-Seq has provided valuable insights into global gene expression in a wide variety of organisms. Using a modified RNA-Seq approach and Illumina's high-throughput sequencing technology, we globally identified 5′-ends of transcripts for the plant pathogen Pseudomonas syringae pv. tomato str. DC3000. A substantial fraction of 5′-ends obtained by this method were consistent with results obtained using global RNA-Seq and 5′RACE. As expected, many 5′-ends were positioned a short distance upstream of annotated genes. We also captured 5′-ends within intergenic regions, providing evidence for the expression of un-annotated genes and non-coding RNAs, and detected numerous examples of antisense transcription, suggesting additional levels of complexity in gene regulation in DC3000. Importantly, targeted searches for sequence patterns in the vicinity of 5′-ends revealed over 1200 putative promoters and other regulatory motifs, establishing a broad foundation for future investigations of regulation at the genomic and single gene levels
Investigation into the reactivity of (h5-C<sub>5</sub>R<sub>5</sub>)M(CO)<sub>2</sub>(alkane) (M = Mn or Re; R = H or Ph; alkane = heptane or cyclopentane) and (h5-C<sub>5</sub>H<sub>5</sub>)Re(CO)<sub>2</sub>(Xe) in solution at cryogenic and room tempera
Tuning the nature of the lowest excited state of [Re(CL)(CO)3(DPPZ-X2)] with X=H, CH3, F, CI, NO2,,COOEt: Combined PS-TRIR and DFT study
Investigation into the reactivity of M(h<sup>5</sup>-C<sub>5</sub>R<sub>5</sub>)(CO)<sub>2</sub>(alkane) (M = Mn or Re; R = H, Me or Ph; alkane = n-heptane or cyclopentane) and Re(h<sup>5</sup>-C<sub>5</sub>H<sub>5</sub>)(CO)<sub>2</sub>(Xe) in solution at cryogenic and room temperature
Picosecond time-resolved infrared investigation into the nature of the lowest excited state of fac-[Re(Cl)(CO)(3)(CO2Et-dppz)] (CO2Et-dppz = dipyrido[3,2a : 2 ',3 ' c]phenazine-11-carboxylic ethyl ester)
Picosecond time-resolved infrared spectroscopic investigation of excited state dynamics in a Pt<sup>II</sup> diimine chromophore
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